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Application of trichoderma asperellum microbial agent in prevention and treatment of crop diseases

A technology for Trichoderma aculeatus and Trichoderma, which is applied in the field of microorganisms, can solve the problems of excessive pesticide residues, residues, and decreased soil organic matter content, and achieves outstanding control effects.

Active Publication Date: 2022-04-01
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical pesticides will leave residues in crops, water and soil, leading to problems such as excessive pesticide residues and environmental pollution.
At the same time, chemical pesticides will also inhibit or even kill other beneficial microorganisms in the soil, reduce the content of soil organic matter, reduce soil fertility, and bring many social, economic and ecological problems

Method used

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  • Application of trichoderma asperellum microbial agent in prevention and treatment of crop diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, purification and identification of Trichoderma aculeatus:

[0029] Scrape 0.5g of sawdust from the wooden comb, add it to 100mL of normal saline to dilute, and spread the bacterial solution with different dilution multiples on the PDA plate (potato 200g / L, glucose 20g / L, agar powder 15-20g / L) and cultured at 28°C for 3-4 days. After the grown colony was streaked and purified for 5 generations, the genome of the strain was extracted and PCR was amplified with ITS1 and ITS4 primers. The sequence was sequenced and compared with the NCBI database. The strain was Trichoderma asperellum.

[0030] The Trichoderma aculeatus colony grew rapidly. When cultured on a PDA plate, within 72 hours, the colony grows white, the center turns light green, and the aerial hyphae are slender and woolly, spreading from the center to the surroundings. After 72-96 hours, the mycelium can cover a PDA plate with a diameter of 9 cm, the center of the colony gradually beco...

Embodiment 2

[0031] Embodiment 2: take corncob as carbon source to the cultivation of Trichoderma asperellum (Trichoderma asperellum)

[0032] PDA plate: potato 200g / L, glucose 20g / L, agar powder 15-20g / L;

[0033] PD medium: potato 200g / L, glucose 20g / L;

[0034] Fermentation medium: corncob 80g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L.

[0035] Take the Trichoderma aculeatus in the seed preservation tube and draw a line on the PDA plate. After separating a single colony, transfer it to the PDA plate and grow it for 96-120 hours. - 200rpm shaker culture for 48h, as a seed solution;

[0036] Add the seed liquid to the fermentation medium according to the inoculation amount of 5% v / v, 28°C, 120r min -1 Shake the culture and adjust the pH to 5.5 every 24 hours. After 7 days of cultivation, the corn cob degradation rate of the Trichoderma aculeatus reaches about 43%. The fermentation broth contains Trichoderma aculeatus thallus, chitinase, glucanase, protease and antagonistic compound...

Embodiment 3

[0037] Embodiment 3: take corn stalk as carbon source to the cultivation of Trichoderma asperellum (Trichoderma asperellum)

[0038] PDA plate: potato 200g / L, glucose 20g / L, agar powder 15-20g / L;

[0039] PD medium: potato 200g / L, glucose 20g / L;

[0040]Fermentation medium: corn straw 80g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 0.3g / L.

[0041] Take the Trichoderma aculeatus in the seed preservation tube and draw a line on the PDA plate. After separating a single colony, transfer it to the PDA plate and grow it for 96-120 hours. - 200rpm shaker culture for 48h, as a seed solution;

[0042] Add the seed liquid to the fermentation medium according to the inoculation amount of 5% v / v, 28°C, 120r min -1 Shake the culture and adjust the pH to 5.5 every 24 hours. After 7 days of cultivation, the corn stalk degradation rate of the Trichoderma aculeatus reaches about 21%. Within 48 hours, Trichoderma aculeatus can grow in the medium with only corn stalks as carbon source, through en...

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Abstract

The invention discloses an application of a Trichoderma asperellum microbial agent in prevention and treatment of crop diseases, the classification name of the strain is Trichoderma asperellum, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20211179, and the preservation date is September 15, 2021. The trichoderma asperellum can inhibit the growth of a plurality of pathogenic fungi, and can effectively prevent and treat crop diseases caused by fusarium oxysporum, gaeumannomyces graminis, cucumber brown spot, watermelon fusarium oxysporum, pumpkin leaf spot, phytophthora capsici, cucumber fusarium oxysporum and the like. The microbial agent has no toxic and harmful effects on the environment and plants, has a certain growth promoting effect on crop growth, and can be applied to preparation of antagonistic bacteria for preventing crop pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a trichoderma aculeatus microbial agent in preventing and treating crop diseases. Background technique [0002] Phytopathogenic fungi can affect the growth of crops. The use of chemical pesticides will leave residues in crops, water and soil, leading to problems such as excessive pesticide residues and environmental pollution. At the same time, chemical pesticides can also inhibit or even kill other beneficial microorganisms in the soil, reduce the content of soil organic matter, reduce soil fertility, and bring many social, economic and ecological problems. With the improvement of living standards and the enhancement of environmental awareness, the creation of green microbial agents to achieve biological control has received more and more attention. [0003] Trichoderma (Trichoderma) is a kind of filamentous fungus ubiquitous in nature. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01P3/00A01P21/00C12N1/14C12R1/885
Inventor 蒋羽佳刘岩松姜岷信丰学章文明姜万奎
Owner NANJING UNIV OF TECH
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