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Application of bergamottin in preparation of antiviral product for treating or preventing novel coronavirus infection

A technology of coronavirus and bergamot, applied in antiviral agents, active ingredients of heterocyclic compounds, food science, etc., to achieve the effect of preventing new coronavirus infection, reducing weight loss, and reducing virus titer

Pending Publication Date: 2022-04-01
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, vaccination is used as the main means of epidemic prevention and control all over the world, but there are differences in the popularity and speed of vaccination in different countries. With the global spread of SARS-CoV-2, the new coronavirus continues to adapt to the host and produces many mutant epidemic strains So far, a total of 5 mutant strains of the new coronavirus have been listed by the World Health Organization as "mutants of close attention", namely Alpha, Beta, Gamma, Delta and the latest Omicron. The rapid mutation of the virus has brought huge challenges to the prevention and control of the epidemic. Challenges, there is an urgent need to develop effective drugs to treat COVID-19

Method used

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  • Application of bergamottin in preparation of antiviral product for treating or preventing novel coronavirus infection
  • Application of bergamottin in preparation of antiviral product for treating or preventing novel coronavirus infection
  • Application of bergamottin in preparation of antiviral product for treating or preventing novel coronavirus infection

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0033] Drugs inhibit SARS-CoV-2 wild-type virus and SARS-CoV-2 mutant virus infection experiment

[0034] Methods: Caco-2 cells were treated with bergamot at concentrations of 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.125 μM, 1.5625 μM and 0.78125 μM for 1 hour, and SARS-CoV-2 wild-type The virus infects the cells at MOI=0.5. After 24 hours of co-incubation of the drug and the virus, the mixture of the drug and the virus is discarded, and the cells in the well are washed with 4% paraformaldehyde to terminate the infection. The nucleoprotein antibody of SARS-CoV-2 is used as The virus is labeled with a specific antibody, and the infection of the virus in the cell is detected by the method of immunofluorescence.

[0035] SARS-CoV-2 popular mutant strains—the British strain (B.1.1.7) and the South African strain (B.1.351) were also tested using the above method.

[0036] See results figure 1 ,according to figure 1 It can be seen that bergamot can effectively inhibit the infection of S...

experiment example 2

[0038] Drug cytotoxicity test

[0039]Method: Use the CCK-8 kit to determine the cytotoxicity of the drug. The WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfobenzene)-2H-tetrazole monosodium salt) can be reduced to highly water-soluble orange-yellow formazan by some dehydrogenases in the mitochondria under the action of the electron coupling carrier 1-MethoxyPMS, generating The color depth of formazan is directly proportional to cell viability and inversely proportional to cytotoxicity. After co-incubating the cells with drugs of different concentration gradients for a corresponding time, WST-8 was added, and the absorbance value was measured at a wavelength of 450 nm using a microplate reader, which can indirectly reflect the number of living cells. Among them, the set concentrations of drugs were 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, and 0.78125 μM, respectively.

[0040] See results figure 2 ,according to figure 2 It can be seen that there is no...

experiment example 3

[0042] The therapeutic effect of drugs on Syrian golden hamsters infected with SARS-CoV-2

[0043] Method: Using Syrian golden hamster as the infection model, the experiment was divided into virus group and treatment group. The hamsters in the treatment group were administered with bergamot 6 hours before infection, and the hamsters received 1×10 5 The SARS-CoV-2 intranasal infection of PFU was infected with the virus. After infection, the hamsters in the treatment group were treated with bergamot essence by gavage, once a day, with a dosage of 75mg / kg. The hamsters in the virus group were gavaged with solvent (corn oil) 6 hours before infection, and the hamsters were treated with 1×10 5 The SARS-CoV-2 nasal drop of PFU was used to infect the virus. After infection, the hamsters in the treatment group were intragastrically administered the solution once a day, and the dosage was 75mg / kg. Continuous administration, observation to the fourth day after infection.

[0044] See ...

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Abstract

The invention relates to the technical field of new application of medicines, in particular to application of bergamottin in preparation of an antiviral product for treating or preventing novel coronavirus infection. According to the present invention, the bergamotin can effectively inhibit the infection of the SARS-CoV-2 wild type virus and the SARS-CoV-2 epidemic mutant strain virus, can reduce the virus titer in the nasal turbinate and the lung tissue, and can reduce the body weight loss degree caused by the infection of the novel coronavirus, such that the bergamotin can be used for treating and / or preventing the novel coronavirus infection, in particular novel coronavirus pneumonia.

Description

technical field [0001] The invention relates to the technical field of new application of medicines, in particular, to the application of bergamot in the preparation of antiviral products for treating or preventing novel coronavirus infection. Background technique [0002] Since the novel coronavirus (SARS-CoV-2) was first isolated from human airway epithelial cells in 2019, SARS-CoV-2, SARS-CoV and MERS-CoV, which cause fatal respiratory diseases in humans, belong to the same category. Nestovirales, Coronaviridae, Betaviruses. The new coronavirus is an enveloped, single-stranded positive-strand RNA virus with a genome size of about 30kb, encoding four main structural proteins: surface spike protein S, envelope glycoprotein E, membrane protein M, and ribonucleoprotein N. Among them, the S protein is a crown-shaped envelope glycoprotein on the surface of the virus particle, which includes the S1 subunit at the N-terminus responsible for binding to the cell receptor angiotens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P31/14A23L33/105
Inventor 王薇肖庚富刘洋周旻旻曹浚垣郭娇
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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