Anti-enzymolysis collagen compound and application thereof

A technology of collagen and peptide collagen, which is applied in the field of anti-enzyme collagen complexes, can solve the problems of unfavorable popularization and use of collagen pharmaceutical products, high cost of collagen pharmaceutical products, and short half-life, so as to promote the improvement of chronic wounds. Effects of healing or tissue repair, reduction of collagenase activity, reduction of decomposition rate

Pending Publication Date: 2022-04-08
尚诚怡美(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The inventor found in the actual use process that the existing collagen pharmaceutical products have at least the following problems: easy to be decomposed, short half-l

Method used

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  • Anti-enzymolysis collagen compound and application thereof
  • Anti-enzymolysis collagen compound and application thereof
  • Anti-enzymolysis collagen compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: Anti-enzymolysis ability test 1

[0062] (1) Dissolve type A gelatin in warm water, prepare 10% (w / v) gelatin solution, add 5% (w / v) ethylenediaminetetraacetic acid disodium salt (hereinafter referred to as EDTA) and stir until dissolved;

[0063] (2) Add 5ml EDTA-gelatin solution to 100ml 45°C preheated soybean oil, stir at 13,000rpm for 15-20 minutes;

[0064] (3) Add ice water and continue to stir for 10-15 minutes, and remove most of the supernatant after standing;

[0065] (4) Wash the EDTA-gelatin microspheres several times with pre-cooled acetone at 4°C and air-dry them;

[0066] (5) 0.5% (w / v) glutaraldehyde was added to EDTA-gelatin microspheres, and cross-linked at 50°C for 6 hours;

[0067] (6) Wash the cross-linked gelatin microspheres several times with purified water, sieve and select 10–50 μm microspheres after drying;

[0068] (7) Dissolve 1g of porcine atelocollagen in 0.1N hydrochloric acid, adjust the pH to pH 7 with 1N NaOH, adjust the...

Embodiment 2

[0073] Embodiment 2: Anti-enzymolysis ability test 2

[0074] (1) Dissolving gelatin in warm water, preparing 10% (w / v) gelatin solution, adding 10% (w / v) ethylenediaminetetraacetic acid disodium salt (hereinafter referred to as EDTA) and stirring until dissolved;

[0075] (2) Add 10ml of EDTA-gelatin solution to 100ml of 55°C preheated mineral oil containing 1% (w / v) sorbitan monooleate, and stir at 2,400rpm;

[0076] (3) After the solution is cooled to room temperature, move the solution to an ice bath and continue to stir, and leave to remove the supernatant;

[0077] (4) Wash the EDTA-gelatin microspheres several times with pre-cooled acetone at 4°C and air-dry them;

[0078] (5) Add 0.5% (w / v) glutaraldehyde solution to EDTA-gelatin microspheres, and cross-link at 40°C for 12 hours;

[0079] (6) Wash the cross-linked gelatin microspheres several times with purified water, sieve and select 10–50 μm microspheres after drying;

[0080] (7) Dissolve 1g bovine atelocollagen...

Embodiment 3

[0085] Embodiment 3: Anti-enzymolysis ability test 3

[0086] (1) Dissolve the recombinant human type III collagen in warm water 0.1N hydrochloric acid, adjust the pH to pH7 with 1N NaOH, prepare 2% (w / v) gelatin solution, add 2.5% (w / v) doxyl hydrochloride Cyclocycline (hereinafter referred to as DOX) is stirred until dissolved;

[0087] (2) Add 5ml DOX-gelatin solution to 100ml soybean oil preheated at 45°C, and stir at 13,000rpm for 15-20 minutes;

[0088] (3) Add ice water and continue to stir for 10-15 minutes, then remove the supernatant after standing still;

[0089] (4) Wash the DOX-gelatin microspheres several times with pre-cooled acetone at 4°C and air-dry them;

[0090] (5) Add 0.5% (w / v) glutaraldehyde to DOX-gelatin microspheres, and cross-link at 50°C for 6 hours;

[0091] (6) Wash the cross-linked gelatin microspheres several times with purified water, sieve and select 10–50 μm microspheres after drying;

[0092] (7) Dissolve 1g of human type I collagen in ...

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Abstract

The invention discloses an anti-enzymolysis collagen compound and application thereof, the anti-enzymolysis collagen compound is composed of polymer microspheres carrying one or more collagenase inhibitors and collagen, the mass ratio of the polymer microspheres to the collagen is 1: 20-20: 1, and the anti-enzymolysis collagen compound can be cross-linked as required. The collagenase inhibitor is carried and slowly released through the polymer microspheres, so that the collagenase activity in a local environment is reduced, the decomposition rate of a collagen product in tissues is reduced, the action time of the collagen product is prolonged, the replacement and use frequency of the collagen product can be reduced, and the use cost of the collagen product is reduced.

Description

technical field [0001] The present invention specifically relates to an anti-enzymolysis collagen compound and its application. The compound is composed of polymer microspheres carrying one or more collagenase inhibitors and collagen. The collagenase inhibitor can prolong collagen The protein exists in the human body for a long time, achieves long-term functions, and has high medical application value. Background technique [0002] Collagen is one of the main protein molecules in the human body, accounting for about 1 / 3 of the total protein in the human body; collagen is also the main component of the extracellular matrix, endowing different tissues with structural properties such as mechanical strength, structure and shape . Among the 28 types of collagen that have been discovered so far, type I collagen has the highest content, followed by type III and type II collagen. Taking type I collagen as an example, its basic unit is the tropocollagen molecule (see appendix figu...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K45/06A61K8/65A61K9/16A61K47/42A61L27/24A61L27/54A61L27/58A61Q19/00A61P17/02
Inventor 周江鸿李同琪徐震梅洪涛李朔
Owner 尚诚怡美(成都)生物科技有限公司
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