Bacterium for enhancing facultative FMBR pesticide wastewater treatment process
A wastewater and phenolic wastewater technology, which is applied in the direction of biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problems of difficult treatment of pesticide wastewater, reduce disposal costs, improve degradation ability, and improve denitrification effect of ability
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Embodiment 1
[0045] The screening of embodiment 1 bacterial strain
[0046] The strain is screened from the bottom mud of a certain river in Yancheng, and the specific screening method is as follows:
[0047] Use 0.300g / L phenol solution and 1g sodium chloride as the screening medium, and add 50mL of river mud taken from a certain river in Yancheng. After 30 days, the phenol was degraded to 0.015g / L. Get the supernatant and inoculate it under a sterile environment until the bacterium colony obtained by the preliminary screening is inoculated into the denitrification medium (potassium nitrate 1.530 3g / L, glucose 3.6g / L, potassium dihydrogen phosphate 0.2g / L, sulfuric acid heptahydrate Magnesium 0.2g / L, calcium carbonate 2g, trace element solution 1mL / L (zinc chloride 80mg, anhydrous copper sulfate 20mg, boric acid 20mg, heptahydrate ferrous sulfate 100mg), water 1000mL, pH7-7.5) medium culture, Measure NH every 12h 4 + -N, NO 3 - -N, total nitrogen content. According to NH 4 + -N, ...
Embodiment 2
[0049] The cultivation of embodiment 2 bacterial strains
[0050] Strain cultivation includes the steps of activation, transfer and expansion,
[0051] Activation: Pick a single colony of phenol-denitrifying bacteria from a solid plate, transfer it to LB liquid medium, shake at a speed of 150 rpm, and culture on a shaker at 35°C for 3-4 hours until the logarithmic phase;
[0052] Transfer: transfer the phenol-denitrification bacterial solution in the logarithmic phase to the seed tank for cultivation, control the temperature of the seed tank at 28°C, maintain the speed at 250rpm, and control the dissolved oxygen DO at 2.5-3mg / L. Cultivate for 24 hours;
[0053] Expansion cultivation: transfer the phenol denitrification bacteria solution cultivated in the seed tank to the fermenter for expansion cultivation according to the inoculation amount of 2%-5%, the medium composition of the fermenter is the same as that of the seed tank, and the physical and chemical parameters are as ...
Embodiment 3
[0058] Get the bacterium liquid of embodiment 2, evenly spread on denitrification medium (0.300g / L phenol, beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, potassium nitrate 1.530 3g / L, glucose 3.6 g / L, potassium dihydrogen phosphate 0.2g / L, magnesium sulfate heptahydrate 0.2g / L, calcium carbonate 2g, trace element solution 1mL / L (zinc chloride 80mg, anhydrous copper sulfate 20mg, boric acid 20mg, sulfuric acid heptahydrate Ferrous iron 100mg), water 1000mL, pH7-7.5), the culture medium was cultured in a constant temperature incubator, the culture temperature was 25°C, the culture time was 24h, the phenol degradation was 0.010g / L, and the phenol degradation rate was 97%. .
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Abstract
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