High-sensitivity specific troponin I latex turbidimetric detection kit adopting transmission-scattering integrated method

A detection kit and high-sensitivity technology are applied in the field of troponin I latex turbidimetric detection kits with a high-sensitivity, specificity-transmission-scattering integrated method, and can solve the problems of incorrect output results of instruments, inconsistent transmission-scattering results, problems, and the like, Achieving the effect of high actual cost, overcoming turbidity defects, and large method throughput

Pending Publication Date: 2022-04-08
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in terms of sensitizer type and concentration selection, surfactant selection, raw materials or formulas that have no problem in transmission will have problems in scattering, resulting in non-specific signal values, resulting in inconsistent transmission and scattering results, and wrong results output by the instrument

Method used

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  • High-sensitivity specific troponin I latex turbidimetric detection kit adopting transmission-scattering integrated method
  • High-sensitivity specific troponin I latex turbidimetric detection kit adopting transmission-scattering integrated method
  • High-sensitivity specific troponin I latex turbidimetric detection kit adopting transmission-scattering integrated method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] The first reagent R1 contains components at the following concentrations:

[0110] 100mM Hepes-NaOH buffer, pH7.5

[0111] NaCl 1600mM

[0112] Polyethylene glycol 12000 3.5g / L

[0113] Tween-20 20ml / L

[0114] Ammonium persulfate 4mM

[0115] Rabbit polyclonal antibody blocking agent 40ml / L (ScantibodyHBR 26)

[0116] Preservative 0.5ml / L PC300

[0117] The corresponding R2 contains the following concentrations of components:

[0118] 50mM Hepes-NaOH buffer pH7.6

[0119] Tween-20 16ml / L

[0120] 480nm carboxyl latex 2.8g / L

[0121] 350nm carboxyl latex 2.6g / L

[0122] Protective agent (sucrose 30g / L, trehalose 10g / L, fructose 10g / L)

[0123] Troponin I rabbit polyclonal antibody 22mL / L (dako)

[0124] SignalUp TM Western detection sensitizer 60mL / L

[0125] Recombinant human serum albumin 9mL / L (wolcavi)

[0126] Preservative 0.5ml / L PC300

[0127] Among them, the carboxyl latex is subjected to secondary convergent polymerization according to 201710986...

Embodiment 2

[0174] The first reagent R1 contains components at the following concentrations:

[0175] 50mM Hepes-NaOH buffer, pH6.8

[0176] NaCl 2000mM

[0177] Polyethylene glycol 12000 3.5g / L

[0178] Tween-20 20ml / L

[0179] Sodium persulfate 6mM

[0180] Rabbit polyclonal antibody blocking agent 20ml / L (Scantibody HBR 26)

[0181] Preservative 0.5ml / L PC300

[0182] The corresponding R2 contains the following concentrations of components:

[0183] 100mM PBS buffer pH7.0

[0184] Tween-20 12ml / L

[0185] 480nm carboxyl latex 1.8g / L

[0186] 350nm carboxyl latex 1.8g / L

[0187] Protective agent (sucrose 20g / L, trehalose 20g / L, fructose 20g / L)

[0188] Troponin I rabbit polyclonal antibody 15mL / L (dako)

[0189] SignalUp TM Western detection sensitizer 80mL / L

[0190] Recombinant human serum albumin 3.5mL / L (wolcavi)

[0191] Preservative 0.5ml / L PC300

[0192] Among them, carboxyl latex is subjected to secondary convergent polymerization according to 2017109869707 "A Me...

Embodiment 3

[0239] The first reagent R1 contains components at the following concentrations:

[0240] 150mM Hepes-NaOH buffer, pH8.0

[0241] NaCl 600mM

[0242] Polyethylene glycol 12000 3.5g / L

[0243] Tween-20 20ml / L

[0244] Potassium persulfate 1mM

[0245] Rabbit polyclonal antibody blocking agent 60ml / L (ScantibodyHBR 26)

[0246] Preservative 1g / L sodium azide

[0247] The corresponding R2 contains the following concentrations of components:

[0248] 200mM Glycine-Tris buffer pH7.3

[0249] Tween-20 4ml / L

[0250] 480nm carboxyl latex 3.5g / L

[0251]350nm carboxyl latex 3.5g / L

[0252] Protective agent (sucrose 10g / L, trehalose 30g / L, fructose 30g / L)

[0253] Troponin I rabbit polyclonal antibody 30mL / L (dako)

[0254] SignalUp TM Western detection sensitizer 40mL / L

[0255] Recombinant human serum albumin 15mL / L (wolcavi)

[0256] Preservative 1g / L sodium azide

[0257] Among them, the carboxyl latex is subjected to secondary convergent polymerization according to...

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Abstract

The invention discloses a high-sensitivity specific troponin I latex turbidimetric detection kit adopting a transmission-scattering integrated method, and belongs to the technical field of biological detection. The invention discloses a high-sensitivity specific troponin I latex turbidimetric detection kit adopting a transmission-scattering integrated method. The kit comprises a first reagent R1 and a second reagent R2, the R1 is prepared from an HEPES-NaOH buffer solution, NaCl, polyethylene glycol 12000, Tween-20, a rabbit polyclonal antibody blocking agent and an oxidizing agent; r2 comprises a buffer solution, tween-20, carboxyl latex of 480 nm, carboxyl latex of 380 nm, a protective agent, a troponin I rabbit polyclonal antibody, a SignalUpTMWestern detection sensitizer and recombinant human serum albumin. The detection rate of healthy people on the surface of the kit is larger than or equal to 95%, the deviation of transmission and scattering results is smaller than 10%, the existing turbidimetric transmission defect is overcome, and the reagent detection rate is remarkably increased.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a high-sensitivity specific transmission and scattering integrated method troponin I latex turbidimetric detection kit. Background technique [0002] Cardiac troponin is a marker that can be detected in the blood after the myocardium is damaged. It is of great value in the diagnosis of acute coronary syndrome (ACS) and risk stratification of heart diseases. It is the first choice for the diagnosis of acute myocardial infarction (AMI) index. The new generation of high-sensitivity troponin I (hs-cTnI) has higher sensitivity, which is helpful for earlier diagnosis of AMI, easier screening of high-risk groups for cardiovascular diseases, and easier preparation and assessment of prognosis. [0003] The "Chinese Expert Consensus on the Clinical Application of Highly Sensitive Detection of Cardiac Troponin" jointly organized by the Chinese Medical Association Cardiovascula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/539G01N33/68G01N21/82
Inventor 陈啸霄王钊
Owner BYRON DIAGNOSTICS SHANGHAI
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