Application of triclabendazole in prevention and treatment of agricultural pathogenic bacteria

A technology of triclabendazole and pathogenic bacteria, applied in applications, animal husbandry, fungicides, etc., to achieve excellent inhibitory effect, wide antibacterial spectrum, and easy synthesis

Pending Publication Date: 2022-04-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the activity of triclabendazole against agricultur

Method used

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  • Application of triclabendazole in prevention and treatment of agricultural pathogenic bacteria
  • Application of triclabendazole in prevention and treatment of agricultural pathogenic bacteria
  • Application of triclabendazole in prevention and treatment of agricultural pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Embodiment 1: the structural formula of triclbendazole is as follows:

[0013]

Example Embodiment

[0014] Example 2: Determination of the activity of triclbendazole against agricultural pathogenic bacteria

[0015] The strains used in this experiment were the strains that were cryopreserved in the laboratory at -80°C with 30% glycerol. The cryopreserved strains were taken out and sterilized in NB solid medium of agricultural bacteria (beef extract: 3 g, peptone: 5 g, yeast powder: 1 g, sucrose: 10 g, agar: 15 g, distilled water: 1 L, pH 7.0; 121 ℃ sterilization 20min) streak above, and incubate at 28°C until a single colony grows. Pick a single colony on the solid medium to agrobacterium NB liquid medium (beef extract: 3g, peptone: 5g, yeast powder: 1g, sucrose: 10g, distilled water: 1L; sterilization at 121°C for 20min) at 28°C, 180rpm Incubate with shaking in a constant temperature shaker until the logarithmic growth phase. Dilute the strain in logarithmic growth phase to about 10 with the corresponding liquid medium 6 CFU / mL for use. The compounds were respectively d...

Example Embodiment

[0028] Example 3: Determination of the activity of triclbendazole against agricultural pathogenic fungi

[0029] The agricultural pathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato agar dextrose medium (PDA for short). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.

[0030] PDA medium configuration method: Wash and peel the potatoes, weigh 200g and cut them into small pieces, boil in distilled water for about 20min (the potato pieces are soft but not rotten), filter with eight layers of gauze, make up the filtrate to 1000mL with distilled water, add 15g of agar , 20g glucose, stir to make it fully dissolved, pack it in a conical flask, sterilize at 121 ℃ for 20 minutes, and cool it for later use. The indoor activity was measured by the mycelial growth rate method.

[0031] Activation of strains: The agricultural pathogenic bacteria were cultured on PDA ...

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Abstract

The invention belongs to the field of medicinal chemistry. The invention discloses a method for preventing and treating triclabendazole, which is caused by agricultural pathogenic bacteria such as rice bacterial leaf blight pathogenic bacteria Xanthomonas oryzae ACCC 11602, citrus canker pathogenic bacteria Xanthomonas axonophora pv.C and potato black shank pathogenic bacteria Pectobacteria ACCC 19901 and agricultural pathogenic fungi such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium graminearum, botrytis cinerea, Magnaporthe oryzae and Phytophthora capsici, wherein the agricultural pathogenic bacteria are caused by the agricultural pathogenic bacteria such as the Sclerotinia sclerotiorum, the rhizoctonia solani, the fusarium graminearum, the Magnaporthe oryzae and the Phytophthora capsici. As a novel agricultural bactericide, triclabendazole has the characteristics of novel structure, broad spectrum and high efficiency, and has the value of being further researched and developed into the novel agricultural bactericide. The structural formula of the compound is shown in the specification.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and discloses that Triclabendazole (Triclabendazole) is used in the prevention and treatment of agricultural pathogenic bacteria Xanthomonas oryzae of rice bacterial blight, citrus canker pathogen Xanthomonas axonopodis pv.Citri and potato black shank pathogen Pectobacterium atroseptica and agricultural New uses of pathogenic fungi Sclerotinia sclerotiorum, Rhizoctonia Solani, Fusarium Graminearum, Botrytis cinerea, Magnaporthe Oryzae and Phytophthora Capsici . Background technique [0002] Triclobendazole is widely used and has good biological activity in insecticide. It has obvious killing effect on Fasciola hepatica of various ages. It is effective against Fasciola hepatica of ruminants such as cattle, sheep and goats, Fasciola hepatica, Fasciola hepatica, Fasciola hepatica, Fasciola hepatica, etc. Triclobendazole is less toxic, and it is also safe and effective when used in combination w...

Claims

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Application Information

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IPC IPC(8): A01N43/52A01P1/00A01P3/00
Inventor 刘映前周勇杨程杰贺颖慧张保琪安俊霞吴争荣罗雄飞马越张智军胡勇梅
Owner LANZHOU UNIVERSITY
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