Smoke tube fungus F21 and application thereof in kitchen waste wastewater treatment

A technology of kitchen waste and tobacco pipe bacteria, applied in the field of microorganisms, to achieve the effects of stable effluent quality, simple operation and management, and simple operation

Pending Publication Date: 2022-04-12
WUHAN INSTITUTE OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no relevant reports on the use of tobacco pipe bacteria in the treatment of kitchen waste wastewater

Method used

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  • Smoke tube fungus F21 and application thereof in kitchen waste wastewater treatment
  • Smoke tube fungus F21 and application thereof in kitchen waste wastewater treatment
  • Smoke tube fungus F21 and application thereof in kitchen waste wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Isolation and purification of bacterial strains

[0047] The live locusts were collected from the farmland of Mantan Village, Jiangcheng Hani and Yi Autonomous County, Pu'er City, Yunnan Province. In a sterile ultra-clean workbench, use a sterilized inoculation needle to pick out the intestines of live locusts, add them to an Erlenmeyer flask containing glass beads and sterile saline, and oscillate to mix evenly. Take the above bacterial solution and dilute it in a 10-fold gradient. Take 100 μL of bacterial solution for each gradient and add it to the PDA plate medium, spread it evenly with a smear glass rod, and separate the different colonies when colonies of appropriate size grow on the plate. Dilute different colonies according to 10-fold gradients, and add 100 μL of each gradient to the casein plate medium, oil plate medium and cellulose Congo red plate medium respectively, and spread it with a smear glass rod Evenly, when a colony of appropriate si...

Embodiment 2

[0052] Example 2: Identification of strain F21

[0053] Morphological identification of strains: In a clean bench, use a sterile puncher with a diameter of 6.0mm to intercept fresh strain F21 bacteria blocks, put them in the center of the PDA medium, and cultivate them at a constant temperature of 28°C. Observe the growth status, shape, color, etc. of the colony in time, and record the horizontal and vertical diameters of the colony. On the 4th day of culture, the hyphae at the edge of the colony were picked and placed on a glass slide, and the morphological characteristics of the mycelia were observed with an optical microscope for morphological identification. For morphological identification, refer to the "Handbook of Fungal Identification".

[0054] Morphological characteristics of strain F21:

[0055] (1) Individual shape: with hyphae and spores, the spores are short rod-shaped.

[0056] (2) Colony traits on the solid medium plate: the hyphae of the colony expanded rap...

Embodiment 3

[0064] Embodiment 3: the preservation of bacterial strain

[0065] The fungal strain Bjerkandera adusta F21 of the genus Nicotiana in Example 2 was preserved. The preservation unit is the China Center for Type Culture Collection, the preservation number is CCTCC No: M20211433, and the preservation date is November 17, 2021.

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Abstract

The invention discloses a nicotiana sp. F21 and application thereof in kitchen waste wastewater treatment, and belongs to the technical field of microorganisms. The bacterial strain is a fuming tube fungus bacterial strain, the fuming tube fungus bacterial strain is Bjerkandra adusta F21, the preservation unit is China Center for Type Culture Collection, the preservation number is CCTCC No: M20211433, and the preservation date is November 17, 2021. The invention also discloses an application of the smokepipe bacterium F21 in kitchen waste wastewater treatment. According to the invention, a strain of nicotiana tabacum F21 is separated and screened from intestinal tracts of locusts in Pu'er City of Yunnan Province, and tests show that the nicotiana tabacum F21 has a good treatment effect on kitchen waste wastewater, the operability of biological treatment of the kitchen waste wastewater is improved, and a new way is provided for biological treatment of the kitchen waste wastewater.

Description

technical field [0001] The invention relates to a pipe fungus F21 and its application in the treatment of kitchen garbage and wastewater, belonging to the technical field of microbes. Background technique [0002] Kitchen waste wastewater is waste water produced by extrusion and rainwater erosion during the collection, transportation, stacking and treatment of food waste. It has the characteristics of high organic content, high COD concentration, and rich trace elements. If it is not treated in time, it will cause great harm to the human body and serious pollution to the environment. Due to its high moisture content and organic matter content, it is very easy to rot and stink and breed mosquitoes and flies in a short period of time, which greatly pollutes the surrounding environment. In addition, kitchen waste wastewater sometimes flows into urban pipe networks or rivers and lakes along with rainwater, which not only affects water quality, but also seriously threatens human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/36C02F9/14C12R1/645C02F1/52C02F1/56C02F3/34C02F103/32
Inventor 张佑红赵小斌陈智涛刘登辉
Owner WUHAN INSTITUTE OF TECHNOLOGY
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