Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus sake for relieving anaphylactic reaction and preparation method thereof

A technology of Lactobacillus sake and allergic reactions, applied in the field of microbes, can solve the problems of losing nutrition, not fundamentally alleviating or controlling wheat dependence-exercise-induced allergic reactions, production and processing effects, etc.

Pending Publication Date: 2022-04-12
ZHEJIANG GONGSHANG UNIVERSITY +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also gluten-removed wheat products today, but these products lose part of their nutrition, and at the same time have an impact on production and processing, and cannot fundamentally alleviate or control wheat dependence-exercise-induced allergic reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus sake for relieving anaphylactic reaction and preparation method thereof
  • Lactobacillus sake for relieving anaphylactic reaction and preparation method thereof
  • Lactobacillus sake for relieving anaphylactic reaction and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The invention provides a kind of Lactobacillus sakei (Lactobacilus sakei), which is used for alleviating wheat dependence-exercise-induced allergic reaction. The Lactobacillus sakei was preserved in China Center for Type Culture Collection on April 12, 2003, and the preservation number is CCTCC DB20082413

[0017] The classification name is: Lactobacilus sakei, and the preservation address is: China. Wuhan. Wuhan University.

[0018] In this embodiment, the Lactobacillus sake is Straight Bacillus tereoides, usually 0.9-1 micron long, single, in pairs or in short chains. Usually lacks flagella, but is able to move. Anaerobic, the surface colony is about micron wide, raised, round, smooth, dense, white, occasionally light yellow or dark yellow. The growth in the culture medium is even very cloudy.

[0019] Further, the Lactobacillus sake is eaten in the form of microcapsules, tablets or freeze-dried powder, and the dosage is 1×10 8 ~10 10 The amount of bacteria in cf...

Embodiment 2

[0023] This example proposes a preparation method of Lactobacillus sake for alleviating allergic reactions for the above examples, including the isolation and identification of bacterial strains, as follows:

[0024] 1. Isolation of strains

[0025] (1) Properly dilute the traditional fermented milk sample with sterile physiological saline, take 100 μL and spread it on MRS (10 g of peptone, 5 g of yeast extract, K 2 HPO 4 2g (including 2.62g of crystal water), 2g of triamine citrate, 20g of glucose, MnSO 4 0.05 (MnSO 4 .H 2 O 0.198g), MgSO 4 0.28g, meat extract 8g, agar 15-20g, NaAc (anhydrous sodium acetate) 5g, (Tween-801mL or 1g), distilled water 1L, Ph7.2, agar medium plate, cultured at 37°C for 48h, Counting, according to the characteristics of the colonies, isolate and purify the strains of the colonies with different characteristics, and preliminarily identify the lactic acid bacteria by G's staining and peroxidase experiment.

[0026] (2) Perform colony morpho...

Embodiment 3

[0034] Lactobacillus sake alleviates wheat dependence-exercise-induced allergic reactions

[0035] (1) Taking 10 WDEIA patients as the research object, the 10 patients were randomly divided into control group (3 people), placebo group (3 people) and probiotics group (4 people). During the test period, subjects in the probiotic group took individually packaged Lactobacillus sake (2g, the number of viable bacteria was 0.5×10 10 CFU / g), the subjects in the placebo group took placebo powder (dried porous dextrin 2g) within 1 hour after lunch every day for 28 consecutive days. All groups were fed the same diet before and during the experiment. Blood samples were collected from the subjects on the 28th day after taking probiotics or placebo, and were subjected to high-speed centrifugation to obtain serum for immune index detection.

[0036] (2) Determination of specific IgE in serum: it was determined by enzyme-linked immunosorbent assay (ELISA). Wheat prolamin was diluted to 15 μg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lactobacillus sake for relieving anaphylactic reaction and a preparation method thereof, and particularly relates to a lactobacillus sake for relieving wheat dependence-exercise induced anaphylactic reaction. The lactobacillus sake is classified and named as lactobacillus sakei, and the lactobacillus sake is named as lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei, lactobacillus sakei and lactobacillus sakei. The lactobacillus sake is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC DB 20082413, and the preservation time is April 12, 2003. The lactobacillus sake disclosed by the invention has the beneficial effects that the lactobacillus sake can regulate the immune function of a body to prevent / treat the wheat-dependent-motion-induced anaphylactic reaction by reducing the content of specific IgE and histamine in serum of a patient suffering from the wheat-dependent-motion-induced anaphylactic reaction.

Description

technical field [0001] The technical field of microorganisms involved in the present invention, in particular, relates to a Lactobacillus sake for alleviating wheat dependence-exercise-induced allergic reactions, a preparation method, a microecological preparation and applications. Background technique [0002] Gluten-related disorders are an emerging worldwide disease with a global prevalence of approximately 5% in the general population. Gluten-related disorders include celiac disease, non-celiac disease, gluten sensitivity, and wheat allergy. Among them is a specific allergic reaction, Wheat-dependent, exercise-induced anaphylax (WDEIA), which occurs specifically after physical exercise after ingestion of wheat. It is characterized clinically by an allergic reaction 1 to 4 hours after wheat ingestion and physical activity, usually with symptoms ranging from localized or systemic nettles to respiratory, gastrointestinal or cardiovascular disease, including angioedema, res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A61K35/747A61P37/08C12R1/225
CPCA61P37/08A23L33/135A61K35/747C12N1/20C12N1/205C12R2001/225A23V2002/00A23V2400/179
Inventor 傅玲琳王彦波王顺余王翀陈剑李洁
Owner ZHEJIANG GONGSHANG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com