Immobilized microbe pellet as well as preparation method and application thereof

A technology for immobilizing microorganisms and pellets, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of being easily affected by external factors, less application, time-consuming, etc., and achieve enhanced stability , Efficient removal, and the effect of preventing concentration reduction

Pending Publication Date: 2022-04-12
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on these advantages, immobilized microorganism technology is applied to the treatment of various organic wastewater, but it is rarely used in the treatment of wastewater containing antibiotics.
At present, the treatment of antibiotic wastewater mostly uses the degradation of microorganisms. Although microorganisms can remove tetracycline well, it takes a long time and is easily affected by external factors.

Method used

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  • Immobilized microbe pellet as well as preparation method and application thereof
  • Immobilized microbe pellet as well as preparation method and application thereof
  • Immobilized microbe pellet as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The strain in the immobilized microbial pellets of the present invention is Alcaligenes sp, which has been sent to the China Center for Typical Culture Collection on December 10, 2020 for preservation, the name is: Alcaligenessp.R3, and the preservation number is CCTCC NO: M2020882 Address: Wuhan University, Wuhan City, Hubei Province, China.

[0041] The described Alcaligenes sp.R3 is enriched from pig manure, separated and purified, and the specific steps are as follows:

[0042] Step 1: Enrichment, separation and purification of strains in immobilized microbial pellets

[0043] Pig manure samples were taken from a pig farm and stored in a -20°C refrigerator in a ziplock bag. Take 10g of the sample and put it into a Erlenmeyer flask containing glass beads, add 90mL of sterile water, shake at 170r / min, 30°C for 20min. Take the treated sample, draw 2mL supernatant and incubate in liquid medium for 2 days; then inoculate in screening medium containing 20mg / L tetracycli...

Embodiment 2

[0050] Preparation method of immobilized microbial pellets

[0051] S1, preparing the microbial strain into a bacterial suspension with a bacterial cell content of 1-9%;

[0052] The specific steps are as follows: Use an inoculation loop to pick the isolated and purified strains and inoculate them into LB liquid medium, place them in a constant temperature shaking incubator at 30°C and 140r / min for cultivation, and after cultivating for 12 hours, take a certain amount of culture solution in a centrifuge Centrifuge at 8000r / min in the tube for 10min, pour out the supernatant, wash with 0.9% normal saline repeatedly for 1-2 times, then dilute with normal saline, measure its absorbance at 600nm with a UV spectrophotometer with normal saline as a reference , and the bacterial solution when the OD600 nm was 1.0 was used as the bacterial suspension, and placed in a 4°C refrigerator for later use.

[0053] S2. Prepare a solution containing sodium carboxymethylcellulose and polydopam...

Embodiment 3

[0058] Determination of sodium carboxymethylcellulose content in immobilized microbial pellets.

[0059] The difficulty of making the immobilized microbial pellets is based on the drop into a spherical shape; calculate the integrity rate of the immobilized microbial pellets, the higher the integrity rate, the greater the mechanical strength of the immobilized microbial pellets, and the integrity rate = complete immobilization The number of immobilized microbial pellets / total number of immobilized microbial pellets×100%. Evaluation of the mechanical strength of the immobilized microbial pellets: 25 prepared immobilized microbial pellets were added to a 250mL Erlenmeyer flask filled with 50mL of water, and stirred at 1200r / min for 1min with a magnetic stirrer. Get 1.5%, 2%, 2.5%, 3%, 3.5% sodium carboxymethylcellulose and be dissolved in the ultrapure water of 100mL, prepare immobilized microorganism pellet according to the method for embodiment 2, pellet according to above-ment...

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Abstract

The invention discloses immobilized microorganism pellets and a preparation method and application thereof.A tetracycline degrading bacterium with high specificity, short time consumption and high removal efficiency is screened out from pig manure, then microorganisms are immobilized on the main components of sodium carboxymethyl cellulose and polydopamine, and the immobilized microorganism pellets are prepared. The method comprises the following steps of: preparing immobilized pellets by using sodium carboxymethyl cellulose and polydopamine, then performing cross-linking action by using a cross-linking agent to obtain the immobilized pellets, and synthesizing the novel tetracycline degradation agent by utilizing the low price, good adhesiveness, hydrophilicity and non-toxicity of sodium carboxymethyl cellulose and polydopamine and the degradation capability of microorganisms on tetracycline. By utilizing the immobilized microorganism pellets, the endurance capacity and the degradation capacity of immobilized microorganisms to toxins in the environment are improved, the microorganisms are fully fixed, the situation that the concentration of the microorganisms is reduced due to the fact that the microorganisms run off along with flowing of water is prevented, the degradation effect is weakened, and the stability and the anti-interference performance of the treatment system are enhanced.

Description

technical field [0001] The invention belongs to the technical field of tetracycline biodegradation by immobilized microbial pellets, and in particular relates to an immobilized microbial pellet and its preparation method and application. Background technique [0002] Antibiotics are a class of secondary metabolites produced by bacteria or other microorganisms. In recent years, for the purpose of treating infectious diseases, antibiotics have been widely used in drug therapy, agricultural and animal husbandry production, and aquaculture. Since the ingested antibiotics cannot be completely metabolized and absorbed, more than 70% of the antibiotics will be excreted with urine and feces, and released into the environment in an active form, and finally released into groundwater and surface soil, which poses a huge threat to human health It also makes it one of the most concerned issues in environmental pollution. Therefore, the pollution caused by residual antibiotics in the en...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12N11/089C12N11/04C02F3/34C12R1/05C02F101/38
Inventor 方俊刘壮壮杨亚博
Owner HUNAN AGRICULTURAL UNIV
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