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Chondroitin sulfate-chitosan multilayer modified betacyanin liposome and preparation method thereof

A technology for chondroitin sulfate and betalain, which is applied in chemical instruments and methods, food forming, and function of food ingredients, etc., can solve problems such as low absorption and utilization, application limitations, etc., and achieve improved structural damage and good stability. , Good slow release effect

Active Publication Date: 2022-04-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]Betatin is a typical representative of natural pigments extracted from food, widely found in dragon fruit, beets and other foods, and its color is bright purple to deep red It is widely used as food coloring, and has good anti-oxidation, anti-tumor, anti-inflammation and other activities; but betalain, like other natural pigments, is easily affected by external environment such as high temperature, pH, ionic strength, etc. It is degraded by the influence of the human body, and its absorption and utilization by the human body are also very low. These factors have limited its application in the food industry

Method used

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  • Chondroitin sulfate-chitosan multilayer modified betacyanin liposome and preparation method thereof
  • Chondroitin sulfate-chitosan multilayer modified betacyanin liposome and preparation method thereof
  • Chondroitin sulfate-chitosan multilayer modified betacyanin liposome and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Weigh 0.6g of soybean lecithin and 0.15g of cholesterol and dissolve it in 12mL of absolute ethanol as the organic phase; take an appropriate amount of Tween 80 and dissolve it in 0.2M PBS by ultrasonic; In PBS, the concentration of betaine was 3 mg / mL, and the concentration of Tween was 12 mg / ml to obtain the aqueous phase. Quickly inject the organic phase into the water phase, where the volume ratio of the organic phase to the water phase is 3:1, and stir in a water bath at 40°C for 30 minutes; then put the solution in a round bottom flask, and remove absolute ethanol on a vacuum rotary evaporator The liposome obtained by ultrasonic probe-membrane processing, wherein the ultrasonic time is 6min, the ultrasonic power is 300W, the ultrasonic treatment mode is 1s on and 1s off, the liposome after the ultrasonic is extruded through the membrane, filtered The membrane diameter is 220nm, and then the prepared liposome is placed in PBS and dialyzed to colorless to obtain NPS...

Embodiment 2

[0038]Weigh 0.12g of soybean lecithin and 0.04g of cholesterol and dissolve it in 12mL of absolute ethanol as the organic phase; take an appropriate amount of Tween 80 and dissolve it in 0.2M PBS by ultrasonic; In PBS, the betalain concentration was 2 mg / mL, and the Tween concentration was 12 mg / ml to obtain an aqueous phase. Quickly inject the organic phase into the water phase, where the volume ratio of the organic phase to the water phase is 3:1, and stir in a water bath at 40°C for 30 minutes; then put the solution in a round bottom flask, and remove absolute ethanol on a vacuum rotary evaporator The liposome obtained by ultrasonic probe-membrane processing, wherein the ultrasonic time is 6min, the ultrasonic power is 300W, the ultrasonic treatment mode is 1s on and 1s off, the liposome after the ultrasonic is extruded through the membrane, filtered The membrane diameter is 220nm, and then the prepared liposome is placed in PBS and dialyzed to colorless to obtain NPS; it i...

Embodiment 3

[0041] The concentration of the chondroitin sulfate aqueous solution was adjusted to 0.05%, and other operations were the same as in Example 2 to prepare SCNPS.

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Abstract

The invention belongs to the technical field of functional food embedding, and provides a chondroitin sulfate-chitosan multilayer modified betacyanin liposome and a preparation method thereof. The betacyanin liposome is prepared through an ethanol injection method, an ultrasonic probe and a membrane passing technology, a large number of sulfate groups (-SO3 <->) on a chondroitin sulfate molecular chain are used as negative electricity groups for the first time, a large number of amino groups (-NH3 < + >) on a chitosan molecular chain are used as positive electricity groups, and the multilayer liposome which is stable in structure and high in bioavailability is constructed through electrostatic interaction. The prepared multi-liposome has a typical core-shell structure, and has a directional slow-release effect on the betacyanin compared with unmodified and monolayer modified liposome, the bioavailability of the betacyanin is improved, and the multi-liposome is not easily and rapidly hydrolyzed and oxidized by gastrointestinal tract enzyme and has better digestion stability.

Description

technical field [0001] The invention belongs to the technical field of functional food embedding, particularly relates to a layer-by-layer electrostatic assembly technology, and further relates to a chondroitin sulfate-chitosan multi-layer modified beet red liposome and a preparation method thereof. Background technique [0002] The natural pigments in food are safe and harmless and have certain biological activity, which has attracted people's attention and attention, but their low stability, easy degradability and low bioavailability have greatly limited their application in food processing. Therefore, the use of embedding technology to construct delivery systems to overcome these defects has become a current research hotspot. [0003] Liposome is one of the most widely used embedding carriers at present. Its structure is vesicle-like, which is composed of phospholipid bilayer. The hydrophilicity and hydrophobicity of phospholipid head and tail endow liposome with unique a...

Claims

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Application Information

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IPC IPC(8): A23P10/30A23L5/10A23L5/43A23L5/30A23L33/125B01F33/45B01J19/10
CPCA23P10/30A23L5/15A23L33/125A23L5/43A23L5/32B01J19/10A23V2002/00A23V2200/32A23V2250/511A23V2300/14A23V2300/48
Inventor 赵振刚宁粤游丽君
Owner SOUTH CHINA UNIV OF TECH
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