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Active peptide, active peptide composition and application of active peptide composition in preparation of products with anti-aging effect

A technology of active peptides and compositions, applied in the field of biomedicine, can solve problems such as aging, and achieve the effect of convenient operation, wide application prospects, and excellent anti-aging activity

Pending Publication Date: 2022-04-29
SHENZHEN KEXING PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no therapeutic products for aging caused by the environmental pollutant benzopyrene

Method used

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  • Active peptide, active peptide composition and application of active peptide composition in preparation of products with anti-aging effect
  • Active peptide, active peptide composition and application of active peptide composition in preparation of products with anti-aging effect
  • Active peptide, active peptide composition and application of active peptide composition in preparation of products with anti-aging effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of active peptide

[0035] (1) Take 200 mg of Fmoc-His Wang Resin and put it in a solid-phase synthesis tube, add N,N-dimethylformamide (DMF) and let it stand for 40 minutes to fully swell the resin, filter off the solvent, and then add piperidine DMF solution, and the solvent was filtered off after shaking. Dissolve Fmoc-Ser-OH, 1-hydroxybenzotriazole, O-benzotriazole-tetramethylurea hexafluorophosphate in DMF, add N,N-diisopropylethylamine and mix well After being protected from light, it has been activated, and added to the resin, stirred with nitrogen at 25° C. for 2 hours, filtered with suction, washed with DMF and dichloromethane in turn, and drained the solvent. Repeat the above steps, add the activated Fmoc-His-OH, Fmoc-Glu-OH, Fmoc-Trp-OH, Fmoc-Leu-OH to the resin in turn, blow and stir with nitrogen at 25°C, wash the resin after the reaction is complete, The obtained filtrate was subjected to rotary evaporation to obtain a precipitate, ...

experiment example

[0043] In order to evaluate the BLO-1, BLO-2, BLO-3 and active peptide composition (the composition of BLO-1, BLO-2 and BLO-3 molar ratio 1:1:1 composition) prepared in Example 1 of the present invention , referred to as TBLO) biological activity, the following effect experiment example. The BaP in the following experimental examples is benzo[a]pyrene.

[0044] (1) Place 10 pregnant adult nematodes on NGM plates, inoculate Escherichia coli strain OP50 and lay eggs for about 5 hours to obtain synchronized populations. Then, BLO-1, BLO-2, BLO-3, TBLO enhanced nematode resistance to oxidative stress was assessed using L4 larval nematodes at 20°C. Nematodes were incubated for 1 day on treated plates containing 0.10 mM BaP, then transferred to NGM / OP50 plates containing 5 mg / mL BLO-1 or BLO-2 or BLO-3 or TBLO.

[0045] (2) Motility was assessed using 1-day-old adult C. elegans at 20°C. C. elegans were cultured on treated plates containing 0.10 mM BaP for 1 day and transferred to...

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PUM

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Abstract

The invention relates to the technical field of biological medicines, and particularly discloses an active peptide, an active peptide composition and application of the active peptide composition in preparation of a product with an anti-aging effect. The active peptide composition comprises a combination of any two or three of active peptides with structures as shown in a formula I, a formula II and a formula III. The active peptide or the composition thereof can relieve oxidative aging caused by environmental pollutants (benzopyrene), has excellent aging delaying activity, and can reduce the generation of lipofuscin. The active peptide or the composition thereof disclosed by the invention has a wide application prospect in preparation of cosmetics, food, dietary supplements or medicines with anti-aging activity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an active peptide, an active peptide composition and its application in preparing a product with anti-aging effect. Background technique [0002] PAHs are one of the major environmental pollutants produced by forest fires, volcanic eruptions, fossil fuel combustion and industry. Benzopyrene (BaP) is a polycyclic aromatic hydrocarbon, which is contained in cigarette smoke and automobile exhaust, and its lipophilicity makes it easy to permeate biological membranes and can also be absorbed through different metabolic pathways. Numerous studies have found that exposure to BaP induces oxidative stress, inflammatory damage, and pulmonary toxicity in bronchial epithelial cells. Oxidative stress is caused by the disruption of the balance between the oxidative and antioxidant systems, which can act through the production of oxygen metabolites (including superoxide anion radicals, hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K8/64A61Q19/08A61K38/08A61P39/06A23L33/18
CPCC07K7/06A61K8/64A61Q19/08A61K38/08A61P39/06A23L33/18A23V2002/00A23V2250/55A23V2200/302
Inventor 田方方马龙鹏罗丹刘杰刘晓宇秦锁富
Owner SHENZHEN KEXING PHARM CO LTD