Lactobacillus reuteri MRD01 and application thereof

A technology of Lactobacillus reuteri and MRD01, applied in the field of microorganisms, can solve the problem of not finding Lactobacillus reuteri to regulate blood sugar, etc., and achieve the effects of excellent hypoglycemic function, relieving constipation, broad market prospects and application value.

Active Publication Date: 2022-04-29
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many side effects when blood sugar is regulated by drugs such as commonly used acarbose, metformin, insulin, etc. Therefore, improving blood sugar levels through dietary therapy and intestinal probiotic regulation is the current key research direction. Lactobacillus reuteri has the effect of regulating blood sugar

Method used

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  • Lactobacillus reuteri MRD01 and application thereof
  • Lactobacillus reuteri MRD01 and application thereof
  • Lactobacillus reuteri MRD01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and identification of Lactobacillus reuteri MRD01 (Lactobacillus reuteri) bacterial strain

[0031] Lactobacillus reuteri MRD01 (Lactobacillus reuteri) strain is isolated from the human intestine, and after isolation, the MRS medium (MRS solid medium includes: 2.0% glucose, 0.2% ammonium citrate, 0.5% sodium acetate, Dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, soil temperature-80, 1%, agar powder 1.5%, adjust pH 6.2~6.6 ) for anaerobic culture, pick strains of different forms for purification, and subculture for 20-30 generations to obtain monoclonal activated strains and store them.

[0032] The morphological characteristics of the strains on the MRS medium were observed: such as figure 1 As shown, the left image is observed under a 100X lens, the middle image is observed under a 400X lens, and the right image is observed on a culture medium. Lactobacil...

Embodiment 4

[0037]Lactobacillus reuteri intervention experiment, according to the weight loss function test method in the "Technical Specifications for Health Food Testing and Evaluation", select C57 / 6J mice with a body weight range of 22 ± 2g (experimental animals and feed are commercially available), 20 in each group , the experiment set up a blank control group, a model control group and five dosage groups. After 10 days of high-sugar and high-fat adaptive feeding, the modeling was successful, and C57 / 6J high-sugar and high-fat model mice were obtained. The blank control group continued to be fed with basic feed , the rest were fed with high-calorie feed (basic feed 80%, lard 10%, egg yolk powder 10%) for 14 days, and 1 / 3 of the obese resistant rats were eliminated by weighing. The mice successfully modeled with high-calorie feed were randomly assigned to 6 Groups, respectively the model control group and No. 1-5 dosage groups, continued to be fed with high-calorie feed, No. 1 dosage gr...

Embodiment 5

[0040] Blood sugar lowering function test: refer to the "Technical Specifications for Health Food Testing and Evaluation" to conduct auxiliary blood sugar lowering function test and evaluation, select C57 / 6J mice with a body weight range of 22 ± 2 g (both experimental animals and feed are commercially available), 20 in each group, The experiment set up a blank control group, a model control group and five dosage groups. After 10 days of high-sugar and high-fat adaptive feeding, the modeling was successful, and C57 / 6J high-sugar and high-fat model mice were obtained. The blank control group continued to be fed with basic feed , the rest were given high-calorie feed (80% basal feed, 10% lard, 10% egg yolk powder) for 10 days, and the mice whose modeling of high-calorie feed was successful continued to be fed with high-calorie feed. After adaptive feeding for 7 days, they were fasted. At 24 hours, give alloxan 45 mg / kg (BW iv) to build a model, fast for 4 hours after 6 days, use a...

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Abstract

The invention relates to the technical field of microorganisms, in particular to lactobacillus reuteri MRD01 and application thereof. The preservation number of the lactobacillus reuteri MRD01 is GDMCC 62017, and research finds that after intervention of the lactobacillus reuteri MRD01, the fat weight of a mouse is reduced, the total weight of the mouse is reduced, and the lactobacillus reuteri MRD01 has the effects of losing weight and the like; meanwhile, the strain also has an excellent hypoglycemic function; the lactobacillus reuteri MRD01 can relieve constipation at the same time, is free of toxic and antigen reactions, can serve as probiotics, and can be prepared into medicines, functional foods or health-care foods with multiple functions of reducing body weight, losing weight and reducing blood sugar.

Description

technical field [0001] The application relates to the technical field of microorganisms, in particular to a strain of Lactobacillus reuteri MRD01 and its application. Background technique [0002] Lactobacillus reuteri is a lactic acid bacterium present in the gut of all vertebrates and mammals. Lactobacillus reuteri has a strong ability to adhere to the intestinal mucosa, which can improve the distribution of intestinal flora, antagonize the colonization of harmful bacteria, and avoid intestinal diseases; Lactobacillus reuteri can produce a species called "reuteri Reuterin" is a non-protein broad-spectrum antibacterial substance that can extensively inhibit the growth of Gram-positive bacteria, Gram-negative bacteria, yeast, fungi, and pathogens. Lactobacillus reuteri is a slightly irregular-shaped campylobacter with rounded ends. Lactobacillus reuteri is an obligate heterofermentative strain that can ferment sugar to produce CO 2 , lactic acid, acetic acid and ethanol. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P3/04A61P3/10A61P1/10C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P3/04A61P3/10A61P1/10A23V2002/00A23V2400/173A23V2200/328A23V2200/332A23V2200/32Y02A50/30
Inventor 韩毛振彭迪张雁
Owner ANHUI MEDICAL UNIV
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