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Compound microorganism wettable powder for preventing and controlling nematodes as well as preparation method and application of compound microorganism wettable powder

A technology of wettable powder and compound microorganisms, which is applied in the field of agricultural microorganisms, can solve the problems of increased dead seedling rate of crops, difficulties in transportation and storage, incompatibility of auxiliaries, etc., to ensure survival and stability, and reduce transportation and storage Cost, the effect of extending storage time

Pending Publication Date: 2022-05-03
QINGDAO YIBAINONG FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, due to the continuous expansion of continuous cropping area and unscientific use of drugs, nematodes have developed and spread faster, their resistance has increased, and the rate of dead seedlings of crops has continued to increase. affected crop production
[0004] There are a variety of nematode control bacterial agents on the market, but due to the single species of bacteria and the incompatibility of additives, it has caused difficulties in transportation and storage, and has disadvantages such as short shelf life.

Method used

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  • Compound microorganism wettable powder for preventing and controlling nematodes as well as preparation method and application of compound microorganism wettable powder
  • Compound microorganism wettable powder for preventing and controlling nematodes as well as preparation method and application of compound microorganism wettable powder
  • Compound microorganism wettable powder for preventing and controlling nematodes as well as preparation method and application of compound microorganism wettable powder

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention is described in detail below in conjunction with specific embodiments.

[0033] Part I: Preparation of Paecilomyces lilacinus powder

[0034] 1. Screen the isolated strains

[0035] The soil collected in Shandong Shouguang Greenhouse was diluted 10 -5 , evenly spread on the PDA medium containing ampicillin antibiotics for isolation and culture, and single fungal colonies were selected.

[0036] 2. Identification of strains

[0037] Using ITS sequence for molecular identification, Paecilomyces lilacinus strains were selected.

[0038] 3. Strain preservation

[0039] Paecilomyces lilacinus WZ004 that we screened and isolated was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing) on ​​March 14, 2017, and the preservation number is CGMCCNo. .13761.

[0040] 4. Cultivate original species

[0041] Paecilomyces lilacinus WZ004 with th...

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Abstract

The invention discloses compound microorganism wettable powder for preventing and controlling nematodes and a preparation method and application thereof.The powder is composed of effective components and auxiliaries, and the effective components comprise paecilomyces lilacinus powder obtained by fermenting paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761 and penicillium oxalicum powder obtained by fermenting penicillium oxalicum WZ006 with the preservation number of CGMCC No.13763; the auxiliary agent is kaolin, a dispersing agent NNO and sodium lignin sulfonate. The invention has the following beneficial effects: (1) the two microbial agents complement each other and can effectively prevent and control nematodes; (2) the affinity is good, and the survivability and stability of spores are ensured; (3) soil-borne diseases can be prevented and treated; and (4) plant growth can be promoted.

Description

[0001] This application is a divisional application with an application date of May 04, 2017, an application number of 201710306254.X, and an invention title of "A Composite Microbial Wettable Powder for Nematode Control and Its Preparation Method". technical field [0002] The invention relates to a microbial preparation and a preparation method thereof, in particular to a compound microbial wettable powder capable of preventing nematodes, a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. Background technique [0003] In recent years, due to the continuous expansion of continuous cropping area and unscientific use of drugs, nematodes have developed and spread faster, their resistance has increased, and the rate of dead seedlings of crops has continued to increase. affected crop production. [0004] There are a variety of bacterial agents for nematode control on the market, but due to the single species of bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N63/36A01N25/14A01P5/00A01P3/00A01P21/00
CPCA01N25/14A01N63/30A01N63/36
Inventor 李文正
Owner QINGDAO YIBAINONG FERTILIZER CO LTD