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Preparation method and application of prodigiosin food composite preservative

A prodigiosin and compound fresh-keeping technology is applied in the preservation, application, and food ingredients of food ingredients as antimicrobials, and achieves the effects of inhibiting respiration, less equipment requirements, and simple operation.

Pending Publication Date: 2022-05-06
SHANDONG FOOD & FERMENT IND RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process condition of the present invention solves the solubility problem of prodigiosin in water insoluble in application, superimposes the antibacterial activity of prodigiosin and resistant dextrin, and the antistaling agent obtained by the combination of the two has stronger antistaling agent Antibacterial activity, thereby promoting the application of prodigiosin and resistant dextrin in the field of food preservation

Method used

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  • Preparation method and application of prodigiosin food composite preservative
  • Preparation method and application of prodigiosin food composite preservative
  • Preparation method and application of prodigiosin food composite preservative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method and application thereof of a prodigiosin food composite preservative, comprising the steps of:

[0031] 1. Production of prodigiosin

[0032] Serratia marcescens in 1.5% glycerol, 1.2% peptone, 0.3% CaCl 2 Prodigiosin was produced by fermentation in a 250 ml Erlenmeyer flask in a fermentation medium with a shaker speed of 200r / min, a pH of 6.3, and a temperature of 34°C.

[0033] 2. Preparation of resistant dextrin solution

[0034] Weigh 0.6g of resistant dextrin, dissolve the resistant dextrin powder in 20ml of water at room temperature, stir and dissolve thoroughly, and prepare a particle-free and transparent 3% resistant dextrin solution.

[0035] 3. Mixing of prodigiosin ethanol solution and resistant dextrin solution

[0036] Mix the pre-prepared 0.5g / L prodigiosin ethanol solution with the resistant dextrin solution in step 1, stir well, see figure 1 .

[0037] 4. Water bath evaporation to remove ethanol

[0038] Heat the mixed solutio...

Embodiment 2

[0045]A preparation method and application thereof of a prodigiosin food composite preservative, comprising the steps of:

[0046] 1. Production of prodigiosin

[0047] Serratia marcescens in 1.5% glycerol, 1.2% peptone, 0.3% CaCl 2 Prodigiosin was produced by fermentation in a 250ml Erlenmeyer flask in a fermentation medium with a shaker speed of 200r / min, a pH of 6.3, and a temperature of 34°C.

[0048] 2. Preparation of resistant dextrin solution

[0049] Weigh 1g of resistant dextrin, dissolve the resistant dextrin powder in 20ml of water at room temperature, stir well to dissolve, and prepare a particle-free and transparent 5% resistant dextrin solution.

[0050] 3. Mixing of prodigiosin ethanol solution and resistant dextrin solution

[0051] Mix the pre-prepared 0.5g / L prodigiosin ethanol solution with the resistant dextrin solution in step 1, and stir evenly.

[0052] 4. Water bath evaporation to remove ethanol

[0053] Heat the mixed solution in step 2 in a water...

Embodiment 3

[0061] A preparation method and application thereof of a prodigiosin food composite preservative, comprising the steps of:

[0062] 1. Production of prodigiosin

[0063] Serratia marcescens in 1.5% glycerol, 1.2% peptone, 0.3% CaCl 2 Prodigiosin was produced by fermentation in a 250ml Erlenmeyer flask in a fermentation medium with a shaker speed of 200 r / min, a pH of 6.3, and a temperature of 34°C.

[0064] 2. Preparation of resistant dextrin solution

[0065] Weigh 0.2g of resistant dextrin, dissolve the powder of resistant dextrin in 20ml of water at room temperature, stir well to dissolve, and prepare a particle-free and transparent 1% resistant dextrin solution.

[0066] 3. Mixing of prodigiosin ethanol solution and resistant dextrin solution

[0067] Mix the pre-prepared 0.5g / L prodigiosin ethanol solution with the resistant dextrin solution in step 1, and stir evenly.

[0068] 4. Water bath evaporation to remove ethanol

[0069] Heat the mixed solution in step 2 in ...

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Abstract

The invention discloses a preparation method and application of a prodigiosin food composite preservative, and the preparation method comprises the following steps: adding a proper amount of water into resistant dextrin, stirring to fully dissolve the resistant dextrin, fully dissolving prodigiosin with ethanol, then adding the prodigiosin into a resistant dextrin solution in an isovolumetric manner, heating in a water bath to remove ethanol in the mixed solution, filtering, and drying to obtain the prodigiosin food composite preservative. The uniformly mixed prodigiosin food composite fresh-keeping agent is obtained.

Description

technical field [0001] The invention relates to the technical field of food preservation and preservation, in particular to a preparation method and application of a prodigiosin food composite preservative. Background technique [0002] Prodigiosin is a natural dark red pigment produced by microorganisms. Its structure is a tripyrrole ring structure. It is easily soluble in organic solvents such as methanol, ethanol, ethyl acetate, and dimethyl sulfoxide, and insoluble in water. Produced by microorganisms such as Bacillus, Riverella, Pseudomonas, and Vibrio. The bacterial strain used in the invention is Serratia marcescens, the pigment has biological activities such as antibacterial, anticancer and antimalarial, and has broad application prospects in the fields of medicine, environmental protection, food and the like. In the field of medicine, prodigiosin can be used as a potential anticancer and antimalarial drug. In the field of food, it can be used as a coloring agent or...

Claims

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Application Information

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IPC IPC(8): A23B7/16
CPCA23B7/16A23V2002/00A23V2200/10A23V2250/5114
Inventor 徐慧冯苗黄艳红田延军朱坤福刘建军王珊珊张兴荣
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST