Hydroxyapatite as well as preparation method and application thereof

A hydroxyapatite and group technology, which is applied in the field of hydroxyapatite with nano-flower morphology and its preparation, can solve the problems of expensive equipment, complicated preparation process, small specific surface area of ​​the product, etc., and achieve excellent biological phase. Capacitive and biodegradable, low cytotoxicity, high endocytic efficiency

Pending Publication Date: 2022-05-06
中科南京绿色制造产业创新研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method provided by the invention solves the disadvantages of expensive hydroxyapatite preparation equipment, high prepara

Method used

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  • Hydroxyapatite as well as preparation method and application thereof
  • Hydroxyapatite as well as preparation method and application thereof
  • Hydroxyapatite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0062] Example 1

[0063] This embodiment provides a preparation method of hydroxyapatite, and the specific steps are as follows:

[0064] (1) Add 0.1ml PDDA (polydiallyl dimethyl ammonium chloride with positive charge on the prepared calcium carbonate surface) to 10ml water and heat it at 80 ℃ to dissolve it completely;

[0065] (2) Add 0.1M CaCl 2 . 2H 2 The solution was stirred vigorously at 1500 rpm for 30 minutes and then added to 1 ml of Nao 2 CO 3 Solution added to CaCl 2 Mix in the solution and stir vigorously for 20s, and finally stand for 3min;

[0066] (3) Obtained particles (expressed as PDDA CaCO 3 ）Wash carefully with water and ethanol for 3 times, and finally vacuum dry for standby.

[0067] (4) Dipeptide hydrogel network system was prepared by experiment:

[0068] First, fmoc-t (120mm) and l-nh 2 (480mm) dissolved in PBS buffer solution (pH 8, 0.1mm, 2ml);

[0069] Take the PDDA CaCO obtained in step (3) 3 80mg ultrasonic mixed in the above solution;

[0070] Fina...

Example Embodiment

[0072] Example 2

[0073] This embodiment provides a preparation method of hydroxyapatite, and the specific steps are as follows:

[0074] (1) Add 0.02ml PSS (sodium polystyrene sulfonate with negative charge on the prepared calcium carbonate surface) to 10ml water and heat it at 80 ℃ to dissolve it completely;

[0075] (2) Add 0.1M CaCl 2 . 2H 2 The solution was stirred vigorously at 1500 rpm for 30 minutes and then added to 1 ml of Nao 2 CO 3 Solution added to CaCl 2 Mix in the solution and stir vigorously for 20s, and finally stand for 3min;

[0076] (3) Obtained particles (expressed as PSS CaCO 3 ）Wash carefully with water and ethanol for 3 times, and finally vacuum dry for standby;

[0077] (4) The dipeptide hydrogel network system was prepared by experiments. Firstly, fmoc-t (120mm) and l-nh 2 (480mm) dissolve in PBS buffer solution (pH 8, 0.1mm, 2ml), and take PSS CaCO obtained in step (3) 3 80mg ultrasonic was mixed in the above solution, and finally 2mg thermolysin enzym...

Example Embodiment

[0079] Example 3

[0080] This embodiment provides a preparation method of hydroxyapatite, and the specific steps are as follows:

[0081] (1) Add 0.2g gelatin (from bovine bone) to 10ml water and heat it at 80 ℃ to dissolve it completely;

[0082] (2) 1m, 1.47g CaCl 2 . 2H 2 O was added to the above gelatin solution, stirred vigorously at 1500 rpm for 30 minutes, and then 1m Na was added 2 CO 3 (dissolved in 1ml water) solution was added to gelatin CaCl 2 Mix in the solution and stir vigorously for 20s, and finally stand for 3min;

[0083] (3) The obtained aragonite calcium carbonate (expressed as b-caco 3 ）Wash carefully with water and ethanol for 3 times, and finally vacuum dry for standby;

[0084] (4) Dipeptide hydrogel network system was prepared by experiment:

[0085] First, fmoc-t and l-nh 2 Dissolved in 2 ml PBS buffer solution (pH 8, 0.1 mm), the molar concentration of fmoc-t in the obtained dipeptide precursor is 120 mm, l-nh 2 The molar concentration of is 480mm;

[0...

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Abstract

The invention provides hydroxyapatite as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving amino acid modified by an Fmoc group and unmodified amino acid in a phosphate buffer solution to obtain a dipeptide precursor solution; the preparation method comprises the following steps: dispersing calcium salt and carbonate in a gelatin aqueous solution, and reacting to obtain vaterite type calcium carbonate; and (2) mixing the dipeptide precursor solution with vaterite type calcium carbonate, condensing amino acid under the catalytic action of enzyme to form dipeptide, settling the vaterite type calcium carbonate in a dipeptide hydrogel network system formed by the dipeptide and carrying out ion exchange reaction, and dissolving the dipeptide to obtain the hydroxyapatite. The hydroxyapatite prepared by the method is loose and porous, has a relatively high specific surface area, can be uniformly dispersed in water, and has important significance for expanding the application range of the hydroxyapatite.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a hydroxyapatite and its preparation method and application, in particular to a hydroxyapatite with nano-flower morphology and its preparation method and application. Background technique [0002] Hydroxyapatite (HAP), the molar ratio of Ca / P is 1.67, Ca 10 (PO 4 ) 6 (OH) 2 , is one of the main mineral components of human and mammalian natural skeletal tissues (such as bones, teeth, etc.), with excellent biocompatibility, biodegradability and mechanical properties, non-toxic and non-immunogenic properties. It plays an important role in tissue engineering, imaging, drug / gene delivery, bone repair, drug release and other fields. [0003] Calcium Carbonate CaCO 3 (Calcium carbonate), also known as limestone, is a white solid, insoluble in water, with a melting point of 1339°C, one of the most common and easily available materials in nature. At the same ti...

Claims

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Application Information

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IPC IPC(8): C01B25/32C01F11/18B82Y40/00A61K47/02A61L27/12A61L27/50A61L27/58
CPCC01B25/32C01F11/181B82Y40/00A61K47/02A61L27/12A61L27/50A61L27/58C01P2006/12C01P2006/14C01P2006/16C01P2004/30C01P2006/22
Inventor 白硕麻宇琦王安河
Owner 中科南京绿色制造产业创新研究院
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