Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hydroxyapatite as well as preparation method and application thereof

A hydroxyapatite and group technology, which is applied in the field of hydroxyapatite with nano-flower morphology and its preparation, can solve the problems of expensive equipment, complicated preparation process, small specific surface area of ​​the product, etc., and achieve excellent biological phase. Capacitive and biodegradable, low cytotoxicity, high endocytic efficiency

Pending Publication Date: 2022-05-06
中科南京绿色制造产业创新研究院 +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method provided by the invention solves the disadvantages of expensive hydroxyapatite preparation equipment, high preparation cost, complicated preparation process, small specific surface area, uneven size and poor dispersion in aqueous solution in the past.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hydroxyapatite as well as preparation method and application thereof
  • Hydroxyapatite as well as preparation method and application thereof
  • Hydroxyapatite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This embodiment provides a method for preparing hydroxyapatite, the specific steps are as follows:

[0064] (1) Take 0.1mL PDDA (polydiallyldimethylammonium chloride, so that the surface of the prepared calcium carbonate has a positive charge) and add it to 10mL water, and heat it at 80°C to dissolve it completely;

[0065] (2) 0.1M CaCl 2 . 2H 2 O was added to the above solution, vigorously stirred at 1500 rpm for 30 min, and then 1 mL of Na 2 CO 3 solution was added to the CaCl 2 Mix in the solution and stir vigorously for 20s, and finally let stand for 3min;

[0066] (3) The obtained particles (expressed as PDDA-CaCO 3 ) were carefully washed three times with water and ethanol respectively, and finally vacuum-dried for later use.

[0067] (4) Preparation of dipeptide hydrogel network system by experiment:

[0068] First Fmoc-T (120mM) and L-NH 2 (480mM) dissolved in PBS buffer solution (pH 8, 0.1mM, 2mL);

[0069] Get the PDDA-CaCO that step (3) obtains 3 ...

Embodiment 2

[0073] Provide a kind of preparation method of hydroxyapatite in the present embodiment, concrete steps are as follows:

[0074] (1) Add 0.02mL PSS (sodium polystyrene sulfonate, which makes the surface of the prepared calcium carbonate negatively charged) into 10mL water, and heat it at 80°C to dissolve it completely;

[0075] (2) 0.1M CaCl 2 . 2H 2 O was added to the above solution, vigorously stirred at 1500 rpm for 30 min, and then 1 mL of Na 2 CO 3 solution was added to the CaCl 2 Mix in the solution and stir vigorously for 20s, and finally let stand for 3min;

[0076] (3) The particles obtained (expressed as PSS-CaCO 3 ) were carefully washed three times with water and ethanol respectively, and finally dried in vacuum for later use;

[0077] (4) Prepare the dipeptide hydrogel network system by experiment, first mix Fmoc-T (120mM) and L-NH 2 (480mM) dissolved in PBS buffer solution (pH 8, 0.1mM, 2mL), take the PSS-CaCO obtained in step (3) 3 80mg ultrasonically ...

Embodiment 3

[0080]Provide a kind of preparation method of hydroxyapatite in the present embodiment, concrete steps are as follows:

[0081] (1) Add 0.2g of gelatin (derived from bovine bone) to 10mL of water, and heat it at 80°C to dissolve it completely;

[0082] (2) 1M, 1.47g CaCl 2 . 2H 2 O was added to the above gelatin solution, vigorously stirred at 1500rpm for 30 minutes, and then 1M Na 2 CO 3 (dissolved in 1 mL of water) solution was added to gelatin CaCl 2 Mix in the solution and stir vigorously for 20s, and finally let stand for 3min;

[0083] (3) The obtained vaterite type calcium carbonate (expressed as B-CaCO 3 ) were carefully washed three times with water and ethanol respectively, and finally dried in vacuum for later use;

[0084] (4) Preparation of dipeptide hydrogel network system by experiment:

[0085] First Fmoc-T and L-NH 2 Dissolved in 2mL PBS buffer solution (pH is 8, 0.1mM), the molar concentration of Fmoc-T in the obtained dipeptide precursor solution is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Specific surface areaaaaaaaaaaa
Apertureaaaaaaaaaa
Pore volumeaaaaaaaaaa
Login to View More

Abstract

The invention provides hydroxyapatite as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving amino acid modified by an Fmoc group and unmodified amino acid in a phosphate buffer solution to obtain a dipeptide precursor solution; the preparation method comprises the following steps: dispersing calcium salt and carbonate in a gelatin aqueous solution, and reacting to obtain vaterite type calcium carbonate; and (2) mixing the dipeptide precursor solution with vaterite type calcium carbonate, condensing amino acid under the catalytic action of enzyme to form dipeptide, settling the vaterite type calcium carbonate in a dipeptide hydrogel network system formed by the dipeptide and carrying out ion exchange reaction, and dissolving the dipeptide to obtain the hydroxyapatite. The hydroxyapatite prepared by the method is loose and porous, has a relatively high specific surface area, can be uniformly dispersed in water, and has important significance for expanding the application range of the hydroxyapatite.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a hydroxyapatite and its preparation method and application, in particular to a hydroxyapatite with nano-flower morphology and its preparation method and application. Background technique [0002] Hydroxyapatite (HAP), the molar ratio of Ca / P is 1.67, Ca 10 (PO 4 ) 6 (OH) 2 , is one of the main mineral components of human and mammalian natural skeletal tissues (such as bones, teeth, etc.), with excellent biocompatibility, biodegradability and mechanical properties, non-toxic and non-immunogenic properties. It plays an important role in tissue engineering, imaging, drug / gene delivery, bone repair, drug release and other fields. [0003] Calcium Carbonate CaCO 3 (Calcium carbonate), also known as limestone, is a white solid, insoluble in water, with a melting point of 1339°C, one of the most common and easily available materials in nature. At the same ti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C01B25/32C01F11/18B82Y40/00A61K47/02A61L27/12A61L27/50A61L27/58
CPCC01B25/32C01F11/181B82Y40/00A61K47/02A61L27/12A61L27/50A61L27/58C01P2006/12C01P2006/14C01P2006/16C01P2004/30C01P2006/22
Inventor 白硕麻宇琦王安河
Owner 中科南京绿色制造产业创新研究院
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com