Composite microbial agent and application thereof in biological reduction of kitchen waste

A technology of compound microbial inoculants and kitchen waste, applied in the field of environmental microorganisms, can solve the problems of few types of efficient and functional microorganisms, limited promotion effect of reduction, and low concentration, so as to achieve reduction and stabilization, and solve biological problems. The effect of low reduction rate and high efficiency degradation

Pending Publication Date: 2022-05-06
上海康鸿凯科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current aerobic bioreduction technology uses the biochemical action of indigenous microorganisms in raw materials to degrade organic matter. There are few types of high-efficiency functional microorganisms, low concentration, and poor activity, which leads to problems such as long degradation cycles for aerobic bioreduction of food waste.
Although some studies have shown that the addition of a certain strain from outside sources can improve the degradation effect of organic matter in kitchen waste, but because the functions of the strains are different, the addition of a single strain can only play a part of the specific functions. Promotional effect is limited

Method used

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  • Composite microbial agent and application thereof in biological reduction of kitchen waste
  • Composite microbial agent and application thereof in biological reduction of kitchen waste
  • Composite microbial agent and application thereof in biological reduction of kitchen waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Isolation, screening and performance determination of kitchen waste bioreduction bacteria

[0042] The isolation and purification process of Geobacillus xylosus CCTCC NO: M 20211626 strain: The test samples were collected from a high-temperature aerobic fermentation tank of a food waste treatment plant, mixed with sterile water, and then diluted in gradients. Take 200 μL of the solution and spread it on the separation and purification medium plate, and culture it in a constant temperature incubator at 50°C for 16 to 24 hours. Pick single colonies with different shapes and sizes, streak and purify, and store them in numbers. A total of 41 strains were obtained after preliminary screening. ;

[0043] The 41 bacterial strains obtained by the preliminary screening were inoculated onto the protease screening medium, amylase screening medium, cellulase screening medium and lipase screening medium respectively by the method of spot connection, and the enzyme producti...

Embodiment 2

[0050] The preparation of embodiment 2 composite microbial bacterial agents

[0051] Preparation of liquid composite microbial agent: Inoculate the pure strains of four strains of Geobacillus caldoxylosilyticus FWGC1, Geobacillus stearothermophilus, Bacillus subtilis and Bacillus smithii respectively in 5mL liquid medium, cultured at 50°C and 180r / min for 12-16h; inoculate the same liquid medium with a volume ratio of 1% to carry out step-by-step expansion culture, after the end of the culture under the same conditions , to obtain the fermentation liquid of four strains of bacteria respectively; the fermentation liquid of the four strains of bacteria is uniformly mixed according to the volume ratio of 1:1:1:1, and the liquid composite microbial bacterial agent can be obtained.

[0052] The liquid medium components are: peptone 3g / L, yeast extract 2g / L, soluble starch 1g / L, NaCl 3g / L, MgCl 2 0.125g / L, CaCl 2 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, trace element solution (Na 2 Mo...

Embodiment 3

[0054] Example 3 The biological reduction treatment effect of compound microbial bacterial agent on different sources of single kitchen waste

[0055] Add solid composite microbial agents to single food waste from different sources. The mass ratio of solid compound microbial agents to food waste is 1:2. After stirring evenly, carry out high-temperature aerobic fermentation at 60°C. During the process, ventilate and stir and proper water replenishment to ensure that the moisture content of the material is 40-60% by weight to ensure uniform heating and under aerobic conditions; the samples of the treatment system are weighed and calculated every 12 hours to obtain the degradation rate of organic matter in kitchen waste, and the reduction The effect is as Figure 1 ~ Figure 3 shown;

[0056] Catering waste is taken from a canteen of a unit, and its organic matter content is about 85.41% by dry weight. figure 1 It is the organic matter degradation rate of the compound microbial ...

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Abstract

The invention relates to a compound microbial agent and application thereof in biological decrement of kitchen waste, the compound microbial agent comprises Geobacillus xylosilylus thermolyticus, Geobacillus stearothermophilus, Bacillus subtilis and Bacillus schlegeli, the Geobacillus xylosilylus thermolyticus is preserved in China Center for Type Culture Collection, the preservation number of the Geobacillus xylosilylus thermolyticus is CGMCC NO.12487, and the preservation number of the Geobacillus xylosilylus thermolyticus is CGMCC NO.1287. The preservation number of the Geobacillus xylosilylus thermolyticus is CGMCC NO.1287. The preservation number of the geobacillus xylosus for pyrolyzing xylose is CCTCC (China Center For Type Culture Collection) NO: M 20211626. The compound microbial agent is used for aerobic organism reduction of the kitchen waste, can quickly and efficiently degrade organic matters in the kitchen waste, and realizes quick reduction and stabilization of the kitchen waste.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a composite microbial bacterial agent for biological reduction of kitchen waste and its application. Background technique [0002] With the rapid development of social economy and the continuous improvement of people's material living standards, the amount of domestic waste is increasing year by year. At present, 46 cities in my country have started waste sorting. With the development and deepening of domestic waste classification, the amount of sorted wet waste (restaurant waste and kitchen waste) has risen sharply, accounting for about 50% of the total domestic waste. ~60%. The garbage has high moisture content, low calorific value, high organic matter content, and is perishable and smelly. If it is not treated effectively, it will easily cause the reproduction of pathogenic bacteria, the breeding of mosquitoes and flies, and water pollution. At the same ti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/02C05F9/04C12R1/01C12R1/125C12R1/07
CPCC12N1/20C12N11/02C05F9/04Y02A40/20Y02W30/40
Inventor 茹凯刘雄伟
Owner 上海康鸿凯科技有限公司
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