Bionic drug-loaded nanoparticles for specific targeting pulsed electric field ablation postoperative inflammatory region and preparation method thereof

Abstract

The invention discloses a bionic drug-loaded nanoparticle for a specific targeted pulsed electric field ablation postoperative inflammatory region and a preparation method. The bionic drug-loaded nanoparticle comprises a drug-loaded liposome and a neutrophile granulocyte membrane coated on the surface of the drug-loaded liposome. The preparation method comprises the following steps: obtaining a neutrophile granulocyte membrane from activated neutrophile granulocytes through an osmotic pressure gradient method, then mixing the neutrophile granulocyte membrane with a drug-loaded liposome, and treating the obtained mixture through a nano extruder to obtain the neutrophile granulocyte membrane drug-loaded nanoparticles. The neutrophile granulocyte membrane targeting drug-loading nanoparticles with targeting ability are prepared by creatively wrapping the neutrophile granulocyte membrane on the surface of the drug-loading liposome by utilizing the characteristics that neutrophile granulocyte has high expelling ability on an inflammation site and can gather on the inflammation site. Based on the principle that neutrophile granulocytes are naturally collected to an inflammatory site after pulsed electric field ablation, a focus in an inflammatory state is targeted, and drugs are released, so that the focus is precisely targeted and treated.

Description

technical field [0001] The application relates to the fields of cell therapy and neutrophil membrane biomimetic nano-medicine, in particular to a biomimetic drug-loaded nano-particle specifically targeting the inflammatory area after pulsed electric field ablation and a preparation method. Background technique [0002] In recent years, with the development of cell electrophysiology, a new type of pulsed electric field ablation technology has emerged. Different from traditional ablation techniques (radiofrequency ablation, microwave ablation, laser ablation, etc.), pulsed electric field ablation acts on the tumor cell membrane through extremely short but strong electrical pulses, forming irreversible "nanoscale" pores on the cell membrane, which leads to the imbalance of cell homeostasis , induce tumor cell apoptosis, and more importantly, the skeleton components that constitute blood vessels, nerves, and pancreaticobiliary ducts can be preserved because they do not contain l...

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Problems solved by technology

[0008] However, since neutrophil membrane biomimetic nanomaterials are generally used for anti-tumor therapy, the existing preparation methods often require a series of activation processes for neutrophil membranes to make them possess immune properties, and the preparation process is relatively complicated, and The activation step is costly

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