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Application of placenta stem cell freeze-dried powder in preparation of medicines and cosmetics

A technology for freeze-dried powder and cosmetics, which is used in cosmetics, cosmetic preparations, drug combinations and other directions to achieve good effects and application value.

Pending Publication Date: 2022-05-13
北京岳淘生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the effect of the freeze-dried powder prepared by the current placental mesenchymal stem cell freeze-drying method needs to be further improved

Method used

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  • Application of placenta stem cell freeze-dried powder in preparation of medicines and cosmetics
  • Application of placenta stem cell freeze-dried powder in preparation of medicines and cosmetics
  • Application of placenta stem cell freeze-dried powder in preparation of medicines and cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The screening of embodiment 1 antifreeze protein

[0027]Firstly, the Marigold officinalis was freeze-dried, ground and crushed, and stirred in 3 times the volume of 10 mmol / L PBS (pH 8.0) for 3 hours. The suspension is centrifuged at 3000r / min for 30min; the supernatant is precipitated with 50% to 100% saturated ammonium sulfate, the salting-out solution is centrifuged at 3000r / min for 30min, the precipitate is dialyzed against pure water overnight, and freeze-dried, called JZH antifreeze crude protein. JZH antifreeze crude protein 100mg was dissolved in 5mL 10mmol / L PBS (pH8.0), and separated by cation exchange column (2.6cm×50cm). The sample volume was 4 mL, and eluted with 10 mmol / L PBS (pH8.0) for 1 h, and then eluted with 0-1.5 mol / L NaCl (containing 10 mmol / L PBS, pH 8.0) for 5 h at a flow rate of 1.0 mL / min. The detection wavelength is 220nm. The active components showing THA were collected, dialyzed against pure water, and freeze-dried. 150mg of lyophilized...

Embodiment 2

[0031] Embodiment 2 Identification of antifreeze characteristics

[0032] For the detection of the activity of Escherichia coli added with antifreeze protein, take freshly activated Escherichia coli liquid, cultivate it until the absorbance OD600 is about 1.0, and dilute it with sterile water for 10 4 times, dispensed into sterilized EP tubes, added the purified antifreeze protein of SEQ ID NO: 1 and mixed with the bacterial solution to a final concentration of 1%, 10%, 20%, and 30% W / v, and at the same time The blank bacterial solution and the bacterial solution added with 20% glycerol were used as the control, and all the experimental groups were placed at -10°C, frozen for 120 hours, 50uL of the bacterial solution was drawn and spread on the LB plate (3 replicates for each group), and incubated upside down at 37°C for 24 hours Afterwards, count the colonies. The result is as figure 2 shown.

[0033] from figure 2 It can be seen from the results that the bacterial surv...

Embodiment 3

[0034] The preparation of embodiment 3 placental stem cells

[0035] Under sterile conditions, the human placental tissue was repeatedly washed with PBS to remove placental blood, and the residual umbilical cord tissue was cut off, and placed in a sterile square dish for use. The steps of the tissue mincing combined with enzyme solution digestion method: ① Firstly, the placental tissue is processed through a tissue mincing separator, and the placenta is crushed to finally obtain placental tissue fragments. ② Place the placental tissue fragments in a 200mL sterile centrifuge tube, and use 0.25% trypsin and 0.1% type I collagenase to digest the placental tissue fragments. ③Digest on a shaker at 37°C for 30 minutes, remove the tissue and retain the digestion solution, centrifuge at 1500r / min for 5 minutes, and collect the cell pellet obtained by centrifugation. ④ The cells collected after centrifugation were resuspended in a-MEM medium containing 10% fetal bovine serum, and cult...

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Abstract

The invention relates to application of placenta stem cell freeze-dried powder in preparation of medicines and cosmetics. The protein with good anti-freezing activity is separated, identified and prepared from the adonis amurensis, and the protein is used for preparing the freeze-dried powder of the stem cells, so that the activity of the stem cells can be protected, and the antioxidant activity can be improved; the prepared freeze-dried powder can be effectively used for preparing medicines for repairing skin injury and cosmetics for resisting skin oxidation, and has better effect and application value.

Description

technical field [0001] The present invention relates to biological field, more specifically relate to the application of placental stem cell freeze-dried powder in cosmetics and medicines. Background technique [0002] Placental mesenchymal stem cells are a type of pluripotent stem cells that can differentiate into various mesenchymal cells such as bone, cartilage, muscle and fat. In the body's normal tissue injury repair process, placental mesenchymal stem cells are an important cell bank involved in tissue regeneration. Under the action of special signals caused by tissue damage, placental mesenchymal stem cells migrate to the damaged site, gather and proliferate locally, and differentiate along different pathways according to different damage signals. Placental mesenchymal stem cells are easy to isolate and expand, and have a strong ability to multiply in vitro. Even if they are expanded 100 million times, they can still maintain their multidirectional differentiation ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61K9/19A61K47/42A61P17/00A61P17/18A61K8/98A61K8/64A61Q19/00A61Q19/08
CPCA61K35/50A61K9/19A61K47/42A61P17/00A61P17/18A61K8/982A61K8/64A61Q19/00A61Q19/08A61K2800/84
Inventor 吕荣取张然
Owner 北京岳淘生物科技有限公司
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