Detection method of penicillin-viridin

Abstract

The invention provides a method for detecting penicillin-viridin. The method comprises the steps of extraction, purification and determination. Wherein the extraction comprises the following steps: centrifuging a to-be-detected sample by adopting an acetonitrile-water solution, taking supernatant liquid, adding n-hexane, centrifuging, and taking subnatant liquid; purification comprises the following steps: purifying the subnatant through a solid-phase extraction column, taking purified liquid nitrogen, blow-drying, and fixing the volume with acetonitrile to obtain a to-be-detected solution; the determination method comprises the following steps: determining the peak area of a to-be-detected solution by adopting a high performance liquid chromatography-quadrupole / electrostatic field orbitrap high resolution mass spectrometry, and calculating the peak area with a concentration-peak area standard working curve of the aflavidin prepared by an external standard method to obtain the concentration of the aflavidin in the to-be-detected sample. An adsorbent of the solid-phase extraction column is a macroporous polymer formed by polymerizing divinylbenzene and N-vinyl pyrrolidone. According to the detection method disclosed by the invention, a cleaner target extract can be obtained, the matrix effect is obviously improved, the recovery rate is high, the test reproducibility is good, the detection accuracy is high, and the trace analysis requirement can be met.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to the technical field of food detection, and more particularly to a detection method for citreoviridin. Background technique [0002] Citreoviridin is one of the common mycotoxins, which has cardiovascular toxicity, neurotoxicity and genotoxicity, and has serious effects on human health. Moldy grains generally contain citreidin, which is the cause of Keshan disease. [0003] At present, the detection of citreogreen penicillin is mainly concentrated in grains, and the extraction techniques involved mainly include liquid-liquid extraction and solid-phase extraction; the detection techniques mainly include colloidal gold immunochromatography and enzyme-linked immunosorbent ELISA kits method, thin-layer chromatography, liquid chromatography, etc. [0004] Extraction methods such as liquid-liquid extraction and solid phase extraction are still insufficient for the extraction of comp...

AI Technical Summary

Problems solved by technology

However, the selectivity and resolution of ordinary mass spectrometry are not high, and the interference of impurities in complex matrices is obvious. A specific extraction and purification technology to reduce the interference of impurities is required, and the detection accuracy of citreoviridin needs to be improved.

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