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Rapid propagation and cultivation method for tissue culture of paphiopedilum delbrueckii

A tissue culture and cultivation method technology, applied in the field of plant tissue culture, can solve problems such as difficulty in large-scale production of seeds and seedlings, lack of large-scale promotion, influence on conservation of Paphiopedilum resources and industrial development, etc. The development of orchid industry and the effect of shortening the cultivation period

Active Publication Date: 2022-05-27
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild species with excellent performance and new breeds selected and bred have not been widely promoted due to the difficulty in large-scale production of seedlings, which has seriously affected the conservation of Paphiopedilum resources and the development of the industry

Method used

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  • Rapid propagation and cultivation method for tissue culture of paphiopedilum delbrueckii
  • Rapid propagation and cultivation method for tissue culture of paphiopedilum delbrueckii
  • Rapid propagation and cultivation method for tissue culture of paphiopedilum delbrueckii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of tissue culture rapid propagation and cultivation method of Paphiopedilum delphinium, comprising the following steps:

[0050] (1) Aseptic sowing:

[0051] Take the 220 DAP (days after pollination) P. delphinium capsules, wipe the surface with a cotton ball dipped in alcohol, rinse with tap water, add neutral detergent to rinse again, soak the pods in alcohol for 1 min, and finally rinse with running water for 15 min Then transfer to the ultra-clean workbench, change the sterile bottle, soak in 75% ethanol for 30s for surface sterilization, rinse with sterile water for 3 times, wash off the surface ethanol, soak in 1% mercuric solution for 10min for sterilization, and rinse with sterile water Wash off the mercuric chloride 5 times, put the pods in a dish and drain the surface water slightly, cut the pods and evenly sprinkle the seeds on the surface of the germination medium; inoculate the seeds in the capsules into the germination medium for cultivation, and th...

Embodiment 2

[0077] A kind of tissue culture rapid propagation and cultivation method of Paphiopedilum delphinium, comprising the following steps:

[0078] (1) Aseptic sowing:

[0079] Take the 220 DAP (days after pollination) P. delphinium capsules, wipe the surface with a cotton ball dipped in alcohol, rinse with tap water, add neutral detergent to rinse again, soak the pods in alcohol for 1 min, and finally rinse with running water for 15 min Then transfer to the ultra-clean workbench, change the sterile bottle, soak in 75% ethanol for 30s for surface sterilization, rinse with sterile water for 3 times, wash off the surface ethanol, soak in 1% mercuric solution for 10min for sterilization, and rinse with sterile water Wash off the mercury chloride 5 times, place the fruit pods in a dish and drain the surface water slightly, cut the fruit pods and evenly sprinkle the seeds on the surface of the germination medium; inoculate the seeds in the capsules into the germination medium for cultiv...

Embodiment 3

[0089] The difference between this example and Example 1 is that the maturity of the P. deshii capsule is 180 DAP, and other steps and parameters are the same as in the example.

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Abstract

The invention relates to the technical field of plant tissue culture, and particularly discloses a rapid propagation and cultivation method for tissue culture of paphiopedilum delbrueckii, which comprises the following steps: (1) sterile seeding; (2) proliferation and subculture; (3) stem tip in-vitro culture; (4) rooting and seedling strengthening culture; (5) seedling hardening; (6) transplanting and growth-promoting cultivation of mycorrhizal fungi; (7) seedling stage management after cultivation. According to the tissue culture rapid propagation and cultivation method for paphiopedilum delbrueckii, a paphiopedilum delbrueckii non-symbiotic germination and asexual cloning tissue culture rapid propagation technical system and a high-speed cultivation technology are established, non-symbiotic germination of paphiopedilum delbrueckii is free of dark treatment, and the growth cycle is shorter; a large number of robust paphiopedilum delbrueckii seedlings can be rapidly cultured, the germination rate of the seedlings is high, the seedlings are robust, and the growth cycle is short; the method is simple and easy to operate and low in production cost, resource protection and sustainable development of rare and endangered wild paphiopedilum delbrueckii are facilitated, and development of the paphiopedilum industry can be well promoted.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture rapid propagation and cultivation method of Paphiopedilum delphinium. Background technique [0002] De's Pocket Orchid, also known as the Vietnamese Beauty Pocket Orchid, is a broad-petaled subgenus of the Orchidaceae Papillon subfamily. It is mainly distributed in Vietnam, China's Guangxi and Yunnan and other places. It is white, the lips are deep cystic, nearly spherical, pink to light purple, with high ornamental value, and it is a good parent material for horticultural breeding. The wild resources of P. chinensis are on the verge of extinction and are listed as critically endangered (CR) species by the International Union for Conservation of Nature (IUCN). At present, there are only about 200 wild plants. Therefore, it is urgent to carry out the protection and artificial breeding of P. chinensis germplasm resources. Work. [0003] L...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G13/00A01G31/00
CPCA01H4/008A01H4/002A01G31/00A01G13/00
Inventor 李秀玲廖宏英范继征王晓国卜朝阳何荆洲曾艳华王丰顺
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI