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Microbial agent for preventing and treating lepidoptera pests of fruits and vegetables as well as preparation method and application of microbial agent

A microbial agent and Lepidoptera technology, which is applied in the field of microbial agent for the prevention and control of Lepidoptera pests in fruits and vegetables and its preparation, can solve the problems of restricting the promotion and use of microbial agents, easy inactivation of microbial agents, unstable storage environment, etc. problem, to achieve the effect of improving UV resistance, improving storage stability, and enhancing insecticidal effect

Active Publication Date: 2022-05-27
赤天化科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the application of existing microbial bacteria in biological control. For example, changes in the field environment and unstable storage environment will easily lead to the inactivation of microbial bacteria, resulting in unstable drug effects and short drug effect cycles. These problems have greatly limited the promotion and use of microbial agents

Method used

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  • Microbial agent for preventing and treating lepidoptera pests of fruits and vegetables as well as preparation method and application of microbial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of titanium-doped crypto-potassium manganese is provided:

[0021] (1) 0.4mol / L KMnO 4 Add 200mL of solution to 1.75mol / L MnSO 4 Solution 70mL and 68% HNO 3 The mixture of solution 7mL was stirred at a speed of 1200-1500r / min, and refluxed at 100°C for 24h, and then cooled to room temperature to obtain a black precipitate, filtered and washed with deionized water until the PH was neutral, then at 120°C Under drying for 24h, octahedral manganese oxide was obtained;

[0022] (2) 2.1 g of titanium tetraisopropoxide was added to 20 mL of 5 mol / L toluene solution, then added to 5.2 g of octahedral manganese oxide, stirred for 20 min, and then the octahedral oxide impregnated with titanium tetraisopropoxide was taken out. The manganese molecular sieve powder was calcined at 500°C for 3 hours, cooled to room temperature, ground and sieved to 154 μm to obtain titanium-doped potassium manganese. The molar amount of Ti in the Ti / Mn molar ratio in the obta...

Embodiment 2

[0024] Provided is a microbial inoculum for controlling lepidopteran pests of fruits and vegetables, the microbial inoculum comprises bacterial powder and titanium-doped cryptopotassium manganese; the bacterial powder includes Bacillus firmus bacteria powder, Verticillium pachyspora bacteria powder, Beauveria bassiana The bacteria powder, the mass ratio of which is 2:3:5; the mass ratio of the bacteria powder and titanium-doped cryptopotassium manganese is 20:1.

Embodiment 3

[0026] Provided is a microbial inoculum for controlling lepidopteran pests of fruits and vegetables, the microbial inoculum comprises bacterial powder and titanium-doped cryptopotassium manganese; the bacterial powder includes Bacillus firmus bacteria powder, Verticillium pachyspora bacteria powder, Beauveria bassiana The bacteria powder, the mass ratio of which is 3:5:7; the mass ratio of the bacteria powder and the titanium-doped cryptopotassium manganese is 15:1.

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Abstract

The invention discloses a microbial agent for preventing and treating lepidoptera pests of fruits and vegetables. The microbial agent comprises bacterial powder and titanium-doped cryptopotassium manganese, the bacterial powder comprises bacillus firmus bacterial powder, verticillium chlamydosporium bacterial powder and beauveria bassiana bacterial powder; the titanium-doped cryptopotassium manganese is prepared by calcining an octahedral manganese oxide molecular sieve and titanium tetraisopropoxide through an impregnation method; the mass ratio of the bacterial powder to the titanium-doped cryptopotassium manganese is (20: 1)-(10: 1); the mass ratio of the bacillus firmus powder to the verticillium chlamydosporium powder to the beauveria bassiana powder is (2-4): (3-7): (5-10); and the Ti molar weight of the Ti / Mn molar ratio in the titanium-doped cryptopotassium manganese is 0.20. The bacillus firmus powder, the verticillium chlamydosporium powder and the beauveria bassiana powder in the microbial agent have a synergistic effect in prevention and control of lepidoptera pests such as plutella xylostella and the like and have a relatively high prevention and control effect, and the activity, the ultraviolet resistance and the storage stability of the microbial agent are enhanced by adding self-made titanium-doped cryptopotassium manganese.

Description

technical field [0001] The invention relates to the technical field of microbial inoculants, in particular to a microbial inoculum for preventing and controlling Lepidoptera pests of fruits and vegetables, and a preparation method and application thereof. Background technique [0002] In recent years, with the in-depth research on the side effects of chemical pesticides on crops and the resistance of pests and bacteria, people are more and more inclined to use microbial agents to resist crop diseases and insect pests. Safety is conducive to good ecological construction. However, the existing microbial bacteria still have many problems in the application of biological control, such as changes in the field environment, unstable storage environment, etc., which may easily lead to the inactivation of microbial bacteria, resulting in unstable efficacy and short efficacy cycle. These problems greatly limit the popularization and use of microbial inoculants. SUMMARY OF THE INVEN...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N63/22A01N25/08A01P7/04
CPCA01N63/30A01N63/22A01N25/08Y02A50/30
Inventor 祝春华祝博伟李梦晴岳增启王长军
Owner 赤天化科技集团有限公司