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Composition and kit for detecting content of lactic acid

The technology of a composition and kit, applied in the field of medical testing, can solve the problems of complex detection methods and occupying many instrument channels, and achieve the effects of high sensitivity, good precision and good accuracy

Pending Publication Date: 2022-05-27
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity of the lactate oxidase method is higher, in order to protect the stability of the chromogen, the dosage form of the reagent used in the lactate oxidase method in the prior art is usually a double reagent, which occupies more instrument channels and the detection method is relatively complicated.

Method used

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  • Composition and kit for detecting content of lactic acid
  • Composition and kit for detecting content of lactic acid
  • Composition and kit for detecting content of lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1 A kind of single-reagent lactic acid detection kit

[0061] 1. Composition

[0062] The components of the kit are shown in Table 1.

[0063] Table 1 A single-reagent lactic acid detection kit

[0064]

[0065] 2. How to use

[0066] (1) The sample does not need pretreatment and can be directly used for detection.

[0067] (2) Manual measurement method:

[0068] Take 2 μL of lactic acid calibrator and mix with 200 μL of kit reagents as a standard sample. Take 2 μL of pure water and mix with 200 μL of reagents of the kit as a blank sample. Take 2 μL of the sample to be tested and mix it with 200 μL of the reagents of the kit as the sample to be tested.

[0069] Incubate the standard sample, blank sample and test sample at 30-37°C for 10 min.

[0070] After incubation, each sample was transferred to a cuvette with a light path of 1.0 cm. Put it into the spectrophotometer, set the main detection wavelength to be 500-546 nm, and the secondary waveleng...

Embodiment 2

[0074] Embodiment 2 A kind of single-reagent lactic acid detection kit

[0075] 1. Composition

[0076] The components of the kit are shown in Table 2.

[0077] Table 2 A single-reagent lactic acid detection kit

[0078]

[0079] 2. How to use

[0080] The specific steps are the same as those in Example 1.

Embodiment 3

[0081] Embodiment 3 A kind of single-reagent lactic acid detection kit

[0082] 1. Composition

[0083] The components of the kit are shown in Table 3.

[0084] Table 3 A kind of single-reagent lactic acid detection kit

[0085]

[0086] 2. How to use

[0087] The specific steps are the same as those in Example 1.

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Abstract

The invention discloses a composition and a kit for detecting the content of lactic acid. The composition contains a buffer solution, a phenol chromogen, 4-aminoantipyrine, peroxidase, lactate oxidase, a stabilizer and a preservative, the kit is a liquid single reagent type kit. Lactic acid is catalyzed by lactic acid oxidase to be oxidized to generate hydrogen peroxide, phenol chromogen and 4-aminoantipyrine generate the quinone colored substance under the action of hydrogen peroxide and peroxidase, and the quinone colored substance is high in molar absorption coefficient and high in detection sensitivity. The single-reagent lactic acid detection kit provided by the invention can be used for rapidly and quantitatively determining the content of lactic acid in human serum or plasma, is good in accuracy, precision and stability, can be stored for at least 14 days after being opened at 2-8 DEG C, and meets the requirements of clinical examination.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular, to a composition and a kit for detecting lactic acid content. Background technique [0002] Lactic acid is an intermediate product of glucose metabolism in the body, mainly produced by red blood cells, striated muscle and brain tissue. The concentration of lactic acid in blood mainly depends on the synthesis rate and metabolic rate of liver and kidney. In some pathological conditions (eg, respiratory failure or circulatory failure), the concentration of lactate in the blood is increased due to tissue hypoxia. In addition, physiological activities such as strenuous exercise and dehydration increase the rate of glycolysis in the process of glucose metabolism in the body, which can also lead to an increase in the concentration of lactic acid in the blood. Elevated lactic acid to a certain level can cause lactic acidosis, which is life-threatening. So by examining blood lacta...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31
Inventor 蒋析文齐文闯吴润锋简俊兴雷苏炜卢华丹
Owner DAAN GENE CO LTD
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