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Method for determining 22 amino acids and taurine in formula food for special medical purpose

A technology for formula food and amino acids, applied in measuring devices, scientific instruments, instruments, etc., can solve the problems of inaccurate detection, undetectable amino acid analysis, incomplete detection, etc., and achieve the effect of improving accuracy

Pending Publication Date: 2022-06-03
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current amino acid analysis has the problems of undetectable, inaccurate and incomplete detection.

Method used

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  • Method for determining 22 amino acids and taurine in formula food for special medical purpose
  • Method for determining 22 amino acids and taurine in formula food for special medical purpose
  • Method for determining 22 amino acids and taurine in formula food for special medical purpose

Examples

Experimental program
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Effect test

Embodiment 1

[0019] 12 kinds of amino acid mixed standard solution internal standard (containing alanine-D 4 ,Phenylalanine- 13 C 6 , Leucine-D 3 , Valine-D 8 , arginine- 13 C-D 4 , Citrulline-D 2 , Glutamate-D 3 , tyrosine- 13 C 6 , ornithine-D 2 , methionine-D 3 , Aspartic acid-D 3 , Glycine- 13 C- 15 N, except glycine- 13 C- 15 Except for N concentration of 2500 μmol / L, other concentrations are 500 μmol / L) Cambridge Isotope Laboratories Inc. Company.

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Abstract

The invention discloses a method for determining 22 amino acids and taurine in formula food for special medical purposes, which comprises the following steps: extracting glutamine, asparagine and tryptophan: adding a streptomyces griseus protease solution into a sample for enzymolysis extraction of glutamine, asparagine and tryptophan, and deriving through AQC; extracting other amino acids and taurine: adding hydrochloric acid (containing mercaptoacetic acid) into a sample for hydrolysis extraction, and deriving through AQC; preparing a standard solution; and determining by adopting liquid chromatography-mass spectrometry. The method for determining the amino acid and the taurine in the UPLC-MS / MS formula food for the special medical purpose is established by adopting an improved acid hydrolysis and innovative enzymolysis technology, reference is provided for formulating detection standards of the amino acid and the taurine in the medical food, and technical support is provided for quality control and supervision of the infant formula food for the special medical purpose.

Description

technical field [0001] The invention belongs to the field of food detection, in particular to a method for determining 22 amino acids and taurine in formula foods for special medical purposes. Background technique [0002] Formula foods for special medical purposes refer to formula foods specially processed and prepared in order to meet the special needs of people with dietary restrictions, digestion and absorption disorders, metabolic disorders or specific disease states for nutrients or diets. As the basic substance of protein required for human nutrition, amino acids are often added to special medical food to promote development and regulate human metabolism. It is one of the main components of medical food that needs key control. In addition, for some amino acid metabolism disorders such as phenylalanine that require medical food, special amino acids are ingredients that must be controlled. The General Rules for Foods for Special Medical Purposes "GB 29922-2013" and "GB...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/7266G01N2030/027G01N2030/045G01N2030/067G01N2030/146
Inventor 卢兰香刘艳明薛霞张艳侠孙立臻程志郑文静王骏祝建华
Owner SHANDONG INST FOR FOOD & DRUG CONTROL