Application of nicotinic glycoside or analogue thereof in preparation of medicine for preventing and treating traumatic brain injury

A technology of nicotinic glycosides and analogs, which is applied in the field of application of nicotinic glycosides or their analogs in the preparation of drugs for the prevention and treatment of traumatic brain injury, which can solve the problems of no reports, etc., and achieve improvement of exercise and reduction of oxidative stress in brain tissue The effect of stimulating and inflammatory factor levels and reducing the degree of cerebral edema

Active Publication Date: 2022-06-07
THE NAVAL MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report on the prevention and treatment effect of nicotinic acid or its analogues on traumatic brain injury.

Method used

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  • Application of nicotinic glycoside or analogue thereof in preparation of medicine for preventing and treating traumatic brain injury
  • Application of nicotinic glycoside or analogue thereof in preparation of medicine for preventing and treating traumatic brain injury
  • Application of nicotinic glycoside or analogue thereof in preparation of medicine for preventing and treating traumatic brain injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of nicotinoid and its analogues

[0035] Preparation of kaempferol, 6-hydroxykaempferol, kaempferol-3-glucoside, kaempferol-3-rutinoside, 6-hydroxykaempferol-3-glucoside, 6-hydroxykaempferol-3 , 6-diglucoside, 6-hydroxykaempferol-3,6,7-triglucoside.

[0036] 35kg of Hefei safflower dried medicinal materials were soaked in 117L of 80% ethanol at room temperature for 48 hours, extracted three times, the extracts were combined, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The petroleum ether extraction part was discarded, and the ether and n-butanol extraction parts were respectively concentrated under reduced pressure and then subjected to column chromatography one by one.

[0037] 1. The ether extract was subjected to silica gel column chromatography, and eluted with petroleum ether-ethyl acetate (30:1-1:1) and chloroform-methanol (10:1-1:1) solvent system as usual (the same below). ), respectively colle...

Embodiment 2

[0042] Example 2: Protective effects of FNS, HKL and KLG on closed diffuse brain injury in rats induced by weight-dropping method

[0043] 1. Test material

[0044] 1.1 Experimental instrument

[0045] Iron stand, PVC pipe, weights.

[0046] 1.2 Test samples

[0047] The purity of pentobarbital sodium, 0.9% saline, FNS, HKL and KLG were all greater than 95.2%. Preparation: Dissolve HKL and KLG in ethanol before use: Tween-80:H 2 O is in a solvent of 1:1:8 for use.

[0048] 2. Experimental animals

[0049] Strain: Male Wistar rat, 260-290 g

[0050] Feeding: constant temperature purification and ventilation animal room, free food and water, 25 ± 2 ℃.

[0051] 3. Grouping and administration

[0052] 84 rats were randomly divided into 7 groups, namely: sham operation group (1), TBI group (2), FNS-L (3), FNS-M (4), FNS-H (5), KLH-H ( 6), KLG-H (7). Among them, the doses corresponding to L, M, and H were 2.5, 5.0, and 10.0 mg / kg / d in the drug treatment groups after TBI mo...

Embodiment 3

[0063] Example 3: Protective effects of FNS, HKL and KLG on brain injury induced by hydraulic shock model in rats

[0064] 1. Test material

[0065] 1.1 Experimental instrument

[0066] STRONG-90 dental drill, ALC-FP6 hydraulic percussion instrument (Alcott Biotech, Shanghai), thermal insulation heating pad, Tissuclyscr-48 multi-sample tissue grinder (Shanghai Jingxin Co., Ltd.), 10S UV spectrophotometer (ThermoFisher Scientific), Pico 17 high-speed Centrifuge (Thermo Fisher Scientific).

[0067] 1.2 Test samples

[0068] Evans blue, formamide (Dalian Meilun Biotechnology Co., Ltd.).

[0069] 2. Experimental animals

[0070] Same as Example 2, the number of animals is required for each part of the experiment.

[0071] 3. Grouping and administration

[0072] Same as Example 2.

[0073] 4. Test method

[0074] 4.1 TBI model establishment

[0075] After being anesthetized with sodium pentobarbital by intraperitoneal injection, the rats were fixed on the heating pad of th...

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PUM

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Abstract

The invention relates to the technical field of medicines, in particular to novel application of nicotinic glycoside (kaempferol-3-O-rutinoside) or analogues thereof in preparation of medicines for preventing and treating traumatic brain injury. The compound has an obvious protection effect on traumatic brain injury of rats, can obviously relieve the encephaledema degree of the rats after injury, improves the behavioral functions after injury, and can improve movement and cognitive impairment and the like within a period of time after injury. Therefore, the compounds represented by nicotinic glycoside can be used for preparing medicines for preventing and treating traumatic brain injury.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, to the application of pyroanthoside or an analog thereof in the preparation of a medicament for preventing and treating traumatic brain injury. Background technique [0002] Traumatic brain injury (TBI) is a mechanical deformation of the skull, cerebral blood vessels, and brain tissue caused by an external force acting on the head, which often leads to temporary or permanent neurological dysfunction. Common causes of injury include traffic accidents, falls or falls, violent attacks, etc., and the incidence is often higher in military operations. [0003] The pathological process of TBI mainly includes two stages: primary injury and secondary injury. Primary damage is directly caused by external forces and can lead to damage to the meninges, including neuronal cell bodies, white matter structures, and vascular beds. Secondary damage occurs for a long time, and refers to a series ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P25/00A61P9/10
CPCA61K31/7048A61P25/00A61P9/10
Inventor 郭美丽张珊珊高越刘程
Owner THE NAVAL MEDICAL UNIV OF PLA
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