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Field inoculation and resistance identification method for crop sclerotiniose pathogenic bacteria sclerotinia sclerotiorum

A technology for Sclerotinia sclerotiorum and Sclerotinia sclerotiorum, which is applied in field inoculation and resistance identification of crop sclerotinia pathogen Sclerotinia sclerotiorum, can solve the problems of inoculation failure, complicated operation, easy shedding, etc., and achieve flexible and precise inoculation site Strain orientation and high inoculation efficiency

Pending Publication Date: 2022-06-17
陕西省杂交油菜研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Field inoculation methods include fungus dish inoculation method, toothpick inoculation method, mycelia ascospore spray method and sclerotia seeding method. Although the results of these several inoculation methods can express the difference between disease-resistant varieties and susceptible varieties to a certain extent, but each has its own limitations
The manual operation of the bacteria plate inoculation method is difficult, labor-intensive and time-consuming. After the inoculation, the bacteria plate is easy to dry and the pathogenic bacteria lose their activity and are easy to fall off, resulting in the failure of the inoculation. The toothpick inoculation method causes great trauma to the crops and cannot truly reflect the resistance level of the material. , and the operation is complicated; mycelium ascospore spray method and seed sclerotia method are limited by some conditions, the inoculation identification is unstable, and it is not suitable for large-scale inoculation identification
At present, there is a lack of a method that is easy to operate and can stably identify crop sclerotinia

Method used

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  • Field inoculation and resistance identification method for crop sclerotiniose pathogenic bacteria sclerotinia sclerotiorum
  • Field inoculation and resistance identification method for crop sclerotiniose pathogenic bacteria sclerotinia sclerotiorum
  • Field inoculation and resistance identification method for crop sclerotiniose pathogenic bacteria sclerotinia sclerotiorum

Examples

Experimental program
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Effect test

preparation example Construction

[0031] In the present invention, the preparation method of the bacteria-carrying grain granules includes the following steps: sequentially soaking and boiling the grain granules to obtain the grain granules to be inoculated, and inoculating the grain granules to be inoculated on the sclerotinia sclerotiorum mycelium. Cultivate in the medium until the surface of the grain particles is covered with Sclerotinia sclerotiorum mycelium to obtain the grain-carrying grain particles.

[0032] In the present invention, the medium is preferably a PDA medium; the temperature of the culture is preferably 20-24°C.

[0033] In the present invention, the soaking time is preferably 20-24 hours; the cooking time is preferably 40-45 min, and the cooking temperature is preferably 80-100°C. After the cooking, it is preferred to also include draining the cooked grains.

[0034] In the present invention, the crops to be inoculated include rape, soybean or sunflower.

[0035] The present invention ...

Embodiment 1

[0044] The purpose of this example is to compare the inoculation method of the present invention with the conventional bacterial dish inoculation method. It mainly includes the efficiency, effect and morbidity of vaccination.

[0045] 1.1 Test material

[0046] 50 copies of Brassica napus provided by the Rape Breeding Room of Shaanxi Hybrid Rape Research Center.

[0047] 1.2 Test method

[0048] 50 portions of Brassica napus each are planted in 8 rows, and each row ensures that at least 25 rapeseeds ready to be inoculated are inoculated by the inoculation method of the present invention and the conventional bacterial dish inoculation method. Each inoculation method was divided into two treatments: water spray and no water spray. Each treatment was inoculated with 50 strains, and the inoculation time was counted. After 3 days of inoculation, the status of the inoculated bacteria, including the existence and existence of pathogenic bacteria, was investigated. morbidity.

[0...

Embodiment 2

[0061] The method of the invention is used for antibacterial sclerotinia breeding of rapeseed, and tens of thousands of materials have been inoculated and screened in the breeding disease garden of the Shaanxi Province Hybrid Rape Research Center since 2000. Inoculate the selected materials with good resistance every year, and continue to inoculate and re-screen in the disease garden in the second year until the materials with good resistance and stability are screened, and then use these materials as parents to cultivate new rapeseed varieties.

[0062] 2.1 Test material

[0063] From 2000 till now, the materials were provided by the Rape Breeding Room of the Shaanxi Hybrid Rape Research Center, and each material was inoculated with 50 plants per year.

[0064] 2.2 Test method

[0065] Inoculation of the present invention: same as Example 1.

[0066] Materials with good resistance were selected during the harvest period, and inoculated in the disease nursery for re-screenin...

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Abstract

The invention belongs to the technical field of crop disease resistance identification, and particularly relates to a field inoculation and resistance identification method of crop sclerotinia sclerotiorum pathogenic bacteria sclerotinia sclerotiorum. According to the method, the paperboard and the fungus-carrying grain particles are connected through the needle-shaped object to form the inoculant, the inoculant is punctured and inoculated to the crop stalks, the fungus-carrying grain particles make contact with the stalks, and rapid and accurate inoculation can be achieved. The field inoculation method of sclerotinia sclerotiorum integrates multiple moisturizing, minimally invasive and high-activity pathogenic bacteria, and is simple and rapid to operate, flexible in inoculation part, capable of accurately determining plant orientation and high in inoculation efficiency, the work efficiency is improved by 48.75% compared with that of a traditional inoculation method, the quality is high, the inoculation success rate is 90% or above, and the method is suitable for large-scale popularization and application. Compared with a traditional inoculation method, the method has the advantages that the moisture content is improved by 56.14% and 120.05% respectively under the conditions of water spraying and moisture non-retention, and the method not only meets the requirement of large-scale inoculation, but also has the advantages which are not achieved by other inoculation methods.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, in particular to a field inoculation and resistance identification method of Sclerotinia sclerotiorum, a pathogen of crop sclerotinia. Background technique [0002] Sclerotinia is a serious fungal disease caused by the parasitic fungus Sclerotiniasclerotiorum (Lib.) deBary. Sclerotinia sclerotiorum is an important plant pathogen with worldwide distribution and a facultative parasitic fungus. Its geographical distribution and host range are very wide. It has been found or prevalent in nearly 100 countries on five continents. According to statistics, its host plants have reached 75 families, 278 genera of 408 species and 42 subspecies or varieties of plants, and the trend of spreading and increasing. Diseases caused by Sclerotinia sclerotiorum pose a serious threat to the yield and quality of important economic crops such as rapeseed, sunflower and soybean. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/18C12R1/645
CPCC12N1/14C12Q1/18
Inventor 李建厂李永红张振兰王灏郭徐鹏王美宁李保军
Owner 陕西省杂交油菜研究中心
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