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Preparation method and application of photoelectrochemical biosensor for detecting oxytetracycline

A biosensor and photoelectrochemical technology, applied in the field of biosensors, can solve the problems of time-consuming and extensive use, false positives, expensive equipment, etc., and achieve the effects of improving detection performance, increasing selectivity, and high sensitivity

Pending Publication Date: 2022-06-28
JIANGSU UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography tandem mass spectrometry, etc., have the advantages of high accuracy and high sensitivity, but their widespread use is limited by factors such as expensive equipment, time-consuming, and professional operations; enzyme-linked immunoassays are simple, fast, and prone to false positives

Method used

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  • Preparation method and application of photoelectrochemical biosensor for detecting oxytetracycline
  • Preparation method and application of photoelectrochemical biosensor for detecting oxytetracycline
  • Preparation method and application of photoelectrochemical biosensor for detecting oxytetracycline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] according to figure 1 Described preparation process:

[0032] (1) Preparation of CN nanomaterials:

[0033] Melamine (2 g) was ground in an agate mortar for 10 min, then placed in an alumina crucible and heated at 500°C for 4h at a ramp rate of 2.3°C / min. After cooling to room temperature in air, the resulting CN powder was collected;

[0034] (2) Bi 2 S 3 - Preparation of CN heterojunction:

[0035] Put Bi(NO 3 ) 3 ·5H 2 O (1.82 g) was dissolved in ethylene glycol (25 mL), followed by Na 2 S·9H 2 O (1.35 g) was dissolved in 30 mL of ultrapure water, then Na 2 S·9H 2 O solution was added dropwise to Bi(NO 3 ) 3 ·5H 2O solution. The CN (12.5 mg) powder prepared in (1) was added rapidly and stirred for 30 min. After stirring, it was transferred to a Teflon-lined autoclave and put into a vacuum drying oven to react at 160 °C for 12 h, and the autoclave was cooled down to room temperature naturally. 2 S 3 -CN heterojunction precipitation, alternately washe...

Embodiment 2

[0040] (1) Preparation of CN nanomaterials:

[0041] Melamine (2 g) was ground in an agate mortar for 10 min, then placed in an alumina crucible and heated at 500°C for 4h at a ramp rate of 2.3°C / min. After cooling to room temperature in air, the resulting CN powder was collected;

[0042] (2) Bi 2 S 3 - Preparation of CN heterojunction:

[0043] Put Bi(NO 3 ) 3 ·5H 2 O (1.82 g) was dissolved in ethylene glycol (25 mL), followed by Na 2 S·9H 2 O (1.35 g) was dissolved in 30 mL of ultrapure water, then Na 2 S·9H 2 O solution was added dropwise to Bi(NO 3 ) 3 ·5H 2 O solution. Quickly add the CN (20mg) powder prepared in (1) and stir for 30min; after stirring, transfer it to a Teflon-lined autoclave and put it into a vacuum drying oven to react at 160°C for 12h, etc. The autoclave was cooled to room temperature naturally, and the Bi 2 S 3 -CN heterojunction precipitation, alternately washed 3 times with ultrapure water and absolute ethanol to remove impurities an...

Embodiment 3

[0048] (1) Preparation of CN nanomaterials:

[0049] Melamine (2 g) was ground in an agate mortar for 10 min, then placed in an alumina crucible and heated at 500°C for 4h at a ramp rate of 2.3°C / min. After cooling to room temperature in air, the resulting CN powder was collected;

[0050] (2) Bi 2 S 3 - Preparation of CN heterojunction:

[0051] Put Bi(NO 3 ) 3 ·5H 2 O (1.82 g) was dissolved in ethylene glycol (25 mL), followed by Na 2 S·9H 2 O (1.35 g) was dissolved in 30 mL of ultrapure water, then Na 2 S·9H 2 O solution was added dropwise to Bi(NO 3 ) 3 ·5H 2 O solution. The CN (25 mg) powder prepared in (1) was added quickly and stirred for 30 min. . After stirring, it was transferred to a Teflon-lined autoclave and put into a vacuum drying oven to react at 160 °C for 12 h, and the autoclave was cooled down to room temperature naturally. 2 S 3 -CN heterojunction precipitation, alternately washed 3 times with ultrapure water and absolute ethanol to remove ...

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Abstract

The invention belongs to the technical field of biosensors, and particularly relates to a preparation method and application of a photoelectrochemical biosensor for detecting oxytetracycline. Bi2S3 with the advantages of good chemical stability, narrow band gap and the like and a CN phase are compounded through a water phase synthesis method to construct a novel high-performance photosensitive composite material-direct Z-type heterojunction, and a photoelectric signal amplification mechanism is achieved; according to the present invention, the terramycin aptamer is further introduced, such that the specific recognition of the terramycin and the aptamer is achieved so as to construct the high-performance photoelectrochemical biosensor, the specific detection of the terramycin is achieved, the sensor has the good linear range within the range of 0.001-1000 nM, and the detection limit is 0.6 pM; the photoelectric biosensor constructed by the invention is low in background signal, high in sensitivity and good in selectivity, and provides a good sensing platform for detecting oxytetracycline.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a preparation method and application of a photoelectrochemical biosensor for detecting oxytetracycline. Background technique [0002] Oxytetracycline (OTC) is a broad-spectrum antibacterial drug, which has a good inhibitory effect on negative bacteria, etc. It is widely used in human medicine, veterinary medicine, fruits and vegetables. The mass fraction of oxytetracycline commonly detected in agricultural soils in China ranges from 0 to 8400 μg·kg -1 , the content of tetracycline antibiotics in the soil of some sample sites exceeded the trigger value (100 μg kg -1 ). Nowadays, there are various technical means to detect oxytetracycline, such as high performance liquid chromatography tandem mass spectrometry, enzyme-linked immunosorbent assay. High performance liquid chromatography tandem mass spectrometry, etc., has the advantages of high accuracy and high sens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/36G01N27/327G01N27/416
CPCG01N27/36G01N27/327G01N27/416
Inventor 刘倩张航姜慧慧杨佩霖由天艳
Owner JIANGSU UNIV
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