Perilla seed oil microemulsion-hydrogel system as well as preparation method and application thereof
A technology of perilla seed oil and microemulsion, which can be used in liquid transportation, emulsion transportation, pharmaceutical formulations, etc. It can solve the problems of loss of effect and instability of encapsulated substances, and achieve good entrapment, slow release and stability Elevated, accessible effects
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[0038] The invention provides a preparation method of a perilla seed oil microemulsion-hydrogel system, comprising the following steps: 1) mixing casein and water to obtain a water phase, mixing EGCG and perilla seed oil to obtain an oil phase, and mixing casein with water to obtain an oil phase; The water phase and the oil phase are mixed and homogenized to obtain a perilla seed oil microemulsion; 2) the gellan gum solution is added dropwise to the chitosan-MgCl 2 In the solution, freeze and thaw repeatedly for 3 to 5 times to obtain a hydrogel; 3) freeze-dry the hydrogel and mix it with the perilla seed oil microemulsion for 10 to 14 hours to obtain a perilla seed oil microemulsion-hydrogel system .
[0039] In the present invention, casein is mixed with water to obtain an aqueous phase, and EGCG is mixed with perilla seed oil to obtain an oil phase. In the present invention, the mass percentage content of the casein in the perilla seed oil microemulsion is preferably 0.4%-...
Embodiment 1
[0050] Preparation of Perilla Seed Oil Microemulsion
[0051] At room temperature of 25°C, 0.5 g of casein was dissolved in 90 mL of deionized water, and ultrasonicated for 5 min as the aqueous phase. Dissolve 0.01 g of EGCG in 10 g of perilla seed oil, and use it as an oil phase after mixing evenly. After mixing the water phase and the oil phase obtained by the above preparation, use a homogenizer at 10,000 rpm for 1 min, for a total of 3 times, to form a coarse emulsion, and then go through a high-pressure homogenization of 500 Mpa for a total of 5 cycles, each 30s; to obtain perilla frutescens Seed oil microemulsion.
[0052] Preparation of hydrogels
[0053] 0.26% (w / v) MgCl 2 An equal volume of the aqueous solution was added dropwise to the chitosan solution (6 g of chitosan in 100 mL of 3% acetic acid solution). The pH of the above solution was adjusted to 3.5 with glacial acetic acid. Gellan gum powder was stirred at 90° C. for 20 min as a 4% (w / v) solution in deio...
experiment example 1
[0057] Detect the particle size, potential and PDI changes of the perilla seed oil microemulsion prepared in Example 1 under different pH conditions, and the particle size, potential, and PDI changes under different NaCl concentration conditions and 1-5 under the condition of 37°C. Changes in MDA content over the course of the day.
[0058] According to the method described in Example 1, without adding EGCG, a CK mixture was prepared.
[0059] The specific method of parameter determination:
[0060] Determination of Average Particle Size of Emulsion
[0061] Take 1 mL of the freshly prepared emulsion into a 10 mL centrifuge tube, add 5 mL of distilled water to dilute, shake evenly, take out 2 mL and put it into a particle size analyzer to measure the average particle size.
[0062] Emulsion Zeta Potential Determination
[0063] Take 1 mL of the freshly prepared emulsion into a 10 mL centrifuge tube, add 5 mL of distilled water to dilute it, shake it evenly, take out 2 mL an...
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