Mycoplasma genitalium parC gene mutation type detection method and kit

Abstract

The invention provides a detection method and a kit for mycoplasma genitalium parC gene mutation types, the detection method is used for detecting eight mutation types of mycoplasma genitalium parC genes, and detection targets of the eight mutation types are (1) ParC S83I, (2) ParC S83C, (3) ParC S83N, (4) ParC S83R, (5) ParC D87G, (6) ParC D87N, (7) ParC D87H and (8) ParC D87Y. The invention further provides reaction primer sequences SEQ ID NO.1 to SEQ ID NO.9 respectively aiming at detection of the eight mutation types. According to the present invention, the 8 mutation types are detected by using the high resolution melting (HRM) technology in combination with the unlabeled probe;

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, and relates to a method for detecting 8 mutation types of Mycoplasma genitalium parC gene, in particular to a method for 8 mutation types of Mycoplasma genitalium parC gene and a kit thereof. Background technique [0002] Mycoplasma genitalium was first isolated in 1980 from samples from two male patients with non-gonococcal urethritis. Mycoplasma genitalium infection accounts for 10-35% of non-chlamydial non-gonococcal urethritis in men. In female patients, M. genitalium is associated with cervicitis and pelvic inflammatory disease (PID). However, in the 10 years following the pathogen's discovery, little progress has been made in determining the clinical importance of M. genitalium in bacteria due to the lack of reliable detection methods. Mycoplasma genitalium, an extremely slow-growing and finicky bacterium, and the new isolates were obtained after a series of techniques...

AI Technical Summary

Problems solved by technology

However, due to the complex mutations at the above two sites (ParCS83I, S83C, S83N, S83R, D87G, D87N, D87H, D87Y), conventional NAAT techniques cannot cover all mutant types

Existing fluorescent quantitative PCR methods need to design porous and multiple probes to cover all mutant types, which greatly increases the cost of these methods in application

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