Mycoplasma genitalium parC gene mutation type detection method and kit
A technology of mycoplasma genitalium and detection kits, which is applied in the field of molecular biology detection, can solve the problems of complex mutations, inability to cover mutant types, and increase costs, and achieve the effects of strong specificity, reduced detection costs, and low cost
Pending Publication Date: 2022-07-05
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
0 Cites 0 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention provides a detection method and a kit for mycoplasma genitalium parC gene mutation types, the detection method is used for detecting eight mutation types of mycoplasma genitalium parC genes, and detection targets of the eight mutation types are (1) ParC S83I, (2) ParC S83C, (3) ParC S83N, (4) ParC S83R, (5) ParC D87G, (6) ParC D87N, (7) ParC D87H and (8) ParC D87Y. The invention further provides reaction primer sequences SEQ ID NO.1 to SEQ ID NO.9 respectively aiming at detection of the eight mutation types. According to the present invention, the 8 mutation types are detected by using the high resolution melting (HRM) technology in combination with the unlabeled probe;
Application Domain
Microbiological testing/measurementMicroorganism based processes +2
Technology Topic
Molecular biologyHigh Resolution Melt Analysis +7
Image
Examples
- Experimental program(1)
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Similar technology patents
Detection method of lithium ion battery diaphram material edge flexibility
Owner:TIANJIN LISHEN BATTERY
Method for detecting mold in food
Owner:WUXI X RES PROD DESIGN & RES
Primer combination for guiding application of beta-receptor blocker, multi-gene detection kit and using method thereof
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD
Method of screening SNP (single nucleotide polymorphism) sites in HSTN (histatherin) gene of cow with mastitis resistance and kit
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
Method and device for controlling replacement of water bucket, and drinking water supply device and storage medium
Owner:FOSHAN SHUNDE MIDEA WATER DISPENSER MFG
Classification and recommendation of technical efficacy words
- Reduce testing costs
- high sensitivity
Machine vision based real-time diagnosis method and system of vegetable leaf diseases
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Array substrate, signal line detection method, display panel and display device
Owner:SHANGHAI TIANMA MICRO ELECTRONICS CO LTD +1
Method for preparing gold-phase section samples aiming at embedded printed-circuit board
Owner:UNIV OF ELECTRONIC SCI & TECH OF CHINA