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BSA gel sterilization material loaded with dihydromyricetin in situ as well as preparation method and application of BSA gel sterilization material

A technology of dihydromyricetin and gel material is applied in the directions of plant raw materials, medical preparations without active ingredients, and medical preparations containing active ingredients, which can solve problems such as poor water solubility and stability, and achieve improved solubility. , The effect of improving drug load and low cost

Active Publication Date: 2022-07-08
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems such as the poor water solubility and stability of dihydromyricetin during use, the purpose of the present invention is to provide a BSA gel sterilizing material with dihydromyricetin loaded in situ, by adding dihydromyricetin The active substances are evenly loaded in the BSA gel material, which improves the solubility of dihydromyricetin. Since dihydromyricetin is embedded in the gel material, dihydromyricetin can be released slowly during the use of the material. It has a bactericidal effect and achieves long-term stable and effective drug effects. The dihydromyricetin contained in the gel bactericidal material is derived from rattan tea, which has broad-spectrum antibacterial properties, and the carrier material BSA is derived from bovine serum, which is in the structure of human serum albumin. It has a similarity of 76%, which improves the biocompatibility of the gel bactericidal material, and the BSA gel has better wetting properties, which is beneficial to the efficacy of dihydromyricetin

Method used

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  • BSA gel sterilization material loaded with dihydromyricetin in situ as well as preparation method and application of BSA gel sterilization material
  • BSA gel sterilization material loaded with dihydromyricetin in situ as well as preparation method and application of BSA gel sterilization material
  • BSA gel sterilization material loaded with dihydromyricetin in situ as well as preparation method and application of BSA gel sterilization material

Examples

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Effect test

Embodiment 1

[0039] An example of a gel sterilizing material of the present invention, the schematic diagram of its formation principle is as follows figure 1 shown. The following are the specific preparation steps of the gel sterilization material:

[0040] (1) Add glutathione (the concentration of glutathione in the solution is 30 mM) to 5 mL of BSA solution (40 wt %), and conduct a reduction reaction at 50° C. for 5 h;

[0041] (2) Take 1 mL of the above solution, add 50 μL of DMSO solution in which 100 mg / mL dihydromyricetin is dissolved, and conduct oxidation reaction at 50° C. for 2 h to prepare a gel antibacterial material.

[0042] The gel bactericidal material prepared in Example 1 was tested for the release rate and release amount of dihydromyricetin, specifically, the gel and the buffer solution were incubated at room temperature, and the released dihydromyricetin in the buffer solution was tested by ultraviolet spectrophotometry. Hydromyricetin amount. from figure 2 It can...

Embodiment 2

[0044] Another example of a gel sterilizing material of the present invention, the specific preparation steps are as follows:

[0045] (1) Glutathione (the concentration of glutathione in the solution is 50 mM) was added to 5 mL of BSA (50 wt %) solution, and a reduction reaction was performed at 40° C. for 6 h.

[0046] (2) Take 1 mL of the above solution, add 50 μL of DMSO solution in which 100 mg / mL dihydromyricetin is dissolved, and carry out oxidation reaction at 40° C. for 3 h to obtain a gel antibacterial material.

[0047] The gel bactericidal material obtained according to the preparation method of this example exhibits a clear gel shape, and its gel properties are consistent with the gel material in Example 1.

Embodiment 3

[0049] The antibacterial performance of the gel antibacterial material prepared in Example 1 was tested. Specifically, the gel antibacterial material was incubated with bacterial liquid containing Escherichia coli or Staphylococcus aureus, and the antibacterial effect of the gel material was judged by observing the growth of bacteria. At the same time, in order to better illustrate the bactericidal properties of the gel material, three groups of control experiments were conducted, namely, pure bacteria incubation without any substances, dihydromyricetin incubation with bacterial liquid, DMSO incubation with bacterial liquid, and other operating conditions were maintained. constant.

[0050] from image 3 It can be seen that in pure bacteria and the agar medium containing DMSO, Escherichia coli and Staphylococcus aureus both grow vigorously, while in the agar medium containing the gel bactericidal material of the present invention or containing dihydromyricetin, Escherichia col...

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Abstract

The invention discloses a BSA (Bovine Serum Albumin) gel sterilization material loaded with dihydromyricetin in situ as well as a preparation method and application of the BSA gel sterilization material. The gel sterilization material is formed by uniformly loading an active substance containing dihydromyricetin in a BSA gel material, and the gel sterilization material utilizes gel of a three-dimensional network structure constructed by BSA to load dihydromyricetin with broad-spectrum sterilization performance in situ, so that the slow release process of dihydromyricetin is facilitated, and the effect of long-term and continuous sterilization is achieved; and the BSA gel sterilization material has good biocompatibility and can be widely applied to clinical preparations.

Description

technical field [0001] The invention relates to a gel bactericidal material, in particular to a BSA gel bactericidal material loaded with dihydromyricetin in situ, and also relates to a preparation method of an in-situ loaded dihydromyricetin gel bactericidal material and the method for preparing the same The invention has the application of bactericidal agents and belongs to the technical field of biomedical materials. Background technique [0002] Bacterial infection is the main cause of many diseases in the human body. For example, intact skin and mucous membranes are natural barriers to prevent bacteria from invading the human body. After damage, bacteria are easy to invade the body from here. Dihydromyricetin, which is mostly extracted from a woody vine plant of the genus Vitaceae, such as vine tea, has various effects such as scavenging free radicals, anti-oxidation, anti-thrombosis, anti-tumor and anti-inflammatory, especially with broad-spectrum bactericidal inhibiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/42A61K47/20A61K47/18A61K31/352A61K36/87A61P31/04
CPCA61K9/06A61K47/42A61K47/20A61K47/183A61K31/352A61K36/87A61P31/04Y02A50/30
Inventor 阳明辉陈翔朱武邓磊夏恬恬
Owner CENT SOUTH UNIV
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