Preparation method of mellea armillaria sporophore antioxidant peptide

A technology of antioxidant peptides and hazel mushrooms, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problem that the antioxidant activity of proteins in hazel mushrooms cannot be known, and achieve the effect of improving the extraction rate

Pending Publication Date: 2022-07-08
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the extraction of the protein from Hazelnut mushroom and the further development and utilization of antioxidant peptides. Therefore, the antioxidant activity of the protein in Hazelnut mushroom is unknown.

Method used

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  • Preparation method of mellea armillaria sporophore antioxidant peptide
  • Preparation method of mellea armillaria sporophore antioxidant peptide
  • Preparation method of mellea armillaria sporophore antioxidant peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of hazelnut mushroom antioxidant peptide, extracting protein according to the following steps:

[0038] (1) Wash the hazel mushrooms, dry them, pulverize them and pass through a 60 mesh sieve, add them into distilled water according to the material-to-liquid ratio of 1:45, after mixing evenly, adjust the pH to 10 with 0.1 mol / L NaOH, and at 80°C Water bath for 1.5h;

[0039] (2) after the alkali extraction is completed, filter out the residue, add distilled water according to the ratio of material to liquid of 1:45, after mixing evenly, add 0.1mol / L NaOH to adjust pH to 9, and ultrasonically treat with 200W power at room temperature for 14-16min;

[0040] (3) Adjust the pH of the supernatant after extraction to 3.7 with 0.1 mol / L hydrochloric acid, then place it at 4°C for 12 hours, and then centrifuge at 10,000 rpm for 20 minutes at this temperature to obtain protein 1, and take the isoelectric point The supernatant after precipitation was added w...

Embodiment 2

[0060] A preparation method of hazelnut mushroom antioxidant peptide is carried out according to the following steps:

[0061] (1) Extraction of protein

[0062] (1) Wash the hazel mushrooms, dry them, pulverize them and pass through a 60 mesh sieve, add them into distilled water according to the material-to-liquid ratio of 1:45, after mixing evenly, adjust the pH to 10 with 0.1 mol / L NaOH, and at 80°C Water bath for 1.5h;

[0063] (2) after the alkali extraction is completed, filter out the residue, add distilled water according to the ratio of material to liquid of 1:45, after mixing evenly, add 0.1mol / L NaOH to adjust pH to 9, and ultrasonically treat with 200W power at room temperature for 14-16min;

[0064] (3) Adjust the pH of the supernatant after the extraction to 3.6 with 0.1 mol / L hydrochloric acid, then place it at 4°C for 12 hours, and then centrifuge at 10,000 rpm for 20 minutes at this temperature to obtain protein 1, and take the isoelectric point The supernat...

Embodiment 3

[0079] Gel Filtration Chromatography:

[0080] Take an appropriate amount of gel Sephadex G-25, add excess distilled water to soak for 24h at room temperature, stir continuously until fully swollen, and pour off the upper floating material. The swollen gel was poured into the column along the inner wall of the column at one time, without bubbles and layers. Equilibrate the column bed with pure water until the baseline is stable. Antioxidant peptides <3kD were prepared into a solution with a concentration of 30mg / mL, sterilized by filtration through a sterile filter, and loaded with 2mL. Elute with pure water, control the flow rate at 2-3mL / 5min, measure the OD254nm value, and collect the samples at the elution peak to obtain component A1 and component A2, which are concentrated and lyophilized.

[0081] Its antioxidant activity was determined. The result is as Image 6 Shown: Both component A1 and component A2 have antioxidant activity in a dose-dependent manner. When the...

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Abstract

According to the preparation method of the mellea armillaria sporophore antioxidant peptide, mellea armillaria sporophore is used as a raw material to be sequentially subjected to protein extraction, precipitation, hydrolysis, separation and purification, the protein extraction comprises the steps that alkali extraction is adopted firstly, then residues obtained after alkali extraction are subjected to ultrasonic alkali extraction, the precipitation comprises the steps that an isoelectric point method and a salting-out method are sequentially carried out for precipitation, and finally drying is carried out to prepare mellea armillaria sporophore protein powder. According to the preparation method disclosed by the invention, through a protein extraction mode of firstly carrying out alkali extraction on the mellea armillaria sporophore and then carrying out ultrasonic alkali extraction, the extraction rate of mellea armillaria sporophore protein is effectively improved and reaches 76.59%, an isoelectric point method and a salting-out method are sequentially combined in a precipitation process, the precipitation amount of the mellea armillaria sporophore protein is effectively promoted to reach 66.2 ug / mL, and the DPPH free radical scavenging rate of the prepared antioxidant polypeptide reaches 86.37%. The scavenging rate of hydroxyl free radicals reaches 97.53% and is close to the scavenging rate of positive control. And the total reducing force is equivalent and reaches 0.71.

Description

technical field [0001] The invention relates to the technical field of preparation of antioxidant peptides, in particular to a preparation method of hazelnut mushroom antioxidant peptides. Background technique [0002] Oxidative damage to the body caused by oxidative free radicals is one of the main causes of human diseases. These diseases include aging, coronary heart disease, inflammation, stroke, diabetes and cancer, etc., posing a great threat to human health. Therefore, the development of antioxidants is of great significance for the treatment and prevention of these diseases. [0003] Antioxidant peptides are favored by researchers as a kind of safe and non-toxic natural antioxidants. They are one of the most popular research topics in the international food industry and a promising functional factor. At present, the preparation of antioxidant peptides mainly uses protease to hydrolyze peptide bonds in proteins, thereby producing peptide substances with antioxidant ac...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30C07K1/14C07K1/16A61K38/01A61P39/06
CPCC12P21/06C07K1/145C07K1/303C07K1/30C07K1/36C07K1/34C07K1/16A61K38/01A61P39/06Y02A40/90
Inventor 李艳丽张倩张文博陈滢竹王刚张斯童陈光
Owner JILIN AGRICULTURAL UNIV
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