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Specific chromatogram construction method and detection method of salvia chinensis medicinal material or standard decoction thereof

A technology of feature map and construction method, which is applied in the field of feature map construction and detection of Ishigami medicinal materials or its standard decoction, can solve the problem of lack of specificity, unable to reflect the basic characteristics of clinical decoction materials, and unable to comprehensively evaluate Ishigami. Wear quality and other issues, to achieve the effect of simple operation, rapid quality evaluation method, good repeatability

Active Publication Date: 2022-07-08
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current Pharmacopoeia standards and local medicinal material standards for Shi Jianchuan are relatively simple, only including traits, microscopic and thin layer identification items, and cannot fully evaluate the quality of Shi Jianchuan
At present, the literature reports on Shi Jianchuan mainly focus on its chemical composition and pharmacological effects, but there are only a few reports on the determination of phenolic acid components in the study of its quality standards.
In addition, Shi Jianchuan has the characteristics of a wide range of sources and complex components. It is mainly used in clinical decoctions. Decoctions usually contain a variety of water-soluble components. It is not enough to rely on simple determination of one or two components. It reflects the inherent quality of Shi Jianchuan medicinal materials and the clinical effects of Shi Jianchuan, lacks specificity, and cannot reflect the material basis characteristics of its clinical decoction

Method used

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  • Specific chromatogram construction method and detection method of salvia chinensis medicinal material or standard decoction thereof
  • Specific chromatogram construction method and detection method of salvia chinensis medicinal material or standard decoction thereof
  • Specific chromatogram construction method and detection method of salvia chinensis medicinal material or standard decoction thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0098] In one example, the preparation method of the reference substance solution includes the following steps:

[0099] Take caffeic acid reference substance, chlorogenic acid reference substance, rosmarinic acid reference substance, and salvianolic acid B reference substance, accurately weigh them, and add methanol aqueous solution with a volume fraction of 40% to 60% to prepare each 1 mL of caffeic acid. 50μg, chlorogenic acid 50μg, rosmarinic acid 100μg, salvianolic acid B 50μg reference solution;

[0100] Take the reference substance of Danshensu sodium, accurately weigh it, and add 40% to 60% methanol aqueous solution to make a reference substance solution containing 50 μg per 1 mL (equivalent to 45 μg of Danshensu per 1 mL).

[0101] In one example, the characteristic map includes 6 characteristic peaks.

[0102] In the present invention, by selecting a suitable reference substance and adopting certain ultra-high performance liquid chromatography (UPLC) conditions, the...

Embodiment 1

[0123] The present embodiment provides a method for constructing a characteristic atlas of Shijianchuan and its standard decoction, which is specifically as follows:

[0124] 1. Instruments, reagents and reagents

[0125] (1) Instruments

[0126] Waters HPLC (Waters H-Class, Waters Corporation), Thermo HPLC (Thermo Vanquish, Thermo Fisher Scientific), Agilent HPLC (Agilent 1290, Agilent); Thermo Scientific TM Q Exactive TM Focus combined quadrupole Orbitrap mass spectrometer (Thermo Fisher, USA); mzVault mass spectrometry database (Thermo Fisher, USA); Waters BEH C18 column (100mm×2.1mm, 1.7μm), YMC Triart C18 column (100mm ×2.1mm, 1.9μm), Agilent SB C18 column (100mm × 2.1mm, 1.8μm); one-thousandth balance (ME204E, METTLER TOLEDO), one-millionth balance (XP26, Mei Teller-Toledo Company); CNC ultrasonic cleaner (KQ500DE, Kunshan Ultrasonic Instrument Co., Ltd.), constant temperature water bath (HWS28, Shanghai Yiheng Technology Co., Ltd.), ultrapure water system (Milli-Q ...

Embodiment 2

[0238] The present embodiment provides a method for detecting the medicinal materials of Shi Jian Chuan

[0239] 1. Instruments, reagents and reagents

[0240] (1) Instruments

[0241] Waters high performance liquid chromatograph (Waters H-Class, Waters Corporation), Waters BEH C18 column (100mm×2.1mm, 1.7μm), 1 / 10,000 balance (ME204E, METTLER TOLEDO), One-millionth balance (XP26, METTLER TOLEDO); CNC ultrasonic cleaner (KQ500DE, Kunshan Ultrasonic Instrument Co., Ltd.), constant temperature water bath (HWS28, Shanghai Yiheng Technology Co., Ltd.), ultra-pure Water system (Milli-Q Direct, Merck & Co.).

[0242] (2) The reagents are the same as those in Example 1.

[0243] (3) The reagent is the same as in Example 1. Among them, the Imitsuba medicinal material numbered S16 in Table 1 was used as the Imitsubishi medicinal material to be tested.

[0244] 2. Preparation of the test solution

[0245] According to the method under "2.3(2)" in Example 1, the test solution of th...

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Abstract

The invention relates to a characteristic spectrum construction method and a detection method of a salvia chinensis medicinal material or a standard decoction thereof. The specific chromatogram construction method comprises the following steps: taking a salvia chinensis reference medicinal material to prepare a reference medicinal material reference solution; taking a caffeic acid reference substance, a chlorogenic acid reference substance, a rosmarinic acid reference substance, a salvianolic acid B reference substance and a salvianic acid A sodium reference substance, and preparing a reference substance solution of the reference substances; preparing a test solution from the salvia chinensis medicinal material or the salvia chinensis standard decoction freeze-dried powder; an ultra-high performance liquid chromatography method is adopted for detection, and a characteristic spectrum is established. According to the specific chromatogram constructed by the method, the characteristic peaks of six water-soluble components are calibrated, the quality monitoring of multiple characteristic components of the salvia chinensis medicinal material can be rapidly and comprehensively realized, a more comprehensive, objective and rapid quality evaluation method is provided for the salvia chinensis medicinal material and a salvia chinensis standard decoction, and the method is simple and convenient to operate and good in repeatability.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for constructing a characteristic map and a detection method of a Shijianchuan medicinal material or its standard decoction. Background technique [0002] The 1977 edition of "Chinese Pharmacopoeia" recorded Ishigami as the dry aerial part of the Lamiaceae plant Salvia chinensis Benth.; harvested during the flowering period in summer and autumn, removed impurities, and dried in the sun; its taste is bitter and acrid. It is calm in nature; it returns to the liver, stomach and lung meridians; it has the functions of clearing away heat and toxin, promoting blood circulation, invigorating qi, and relieving pain; it is used for distending pain in the abdomen and flanks, and carbuncle swelling. The chemical components of Iwami Chuan are mainly phenolic acids, phenolic esters and triterpenes, and also include acidic polysaccharides, sterols, amino acids, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86G01N30/04
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N30/8679G01N2030/047Y02A50/30
Inventor 梁慧陶晨璐范倩彭致铖刘艳梅蔡盛康吴晓纯丁青朱德全
Owner GUANGDONG YIFANG PHARMA