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Preparation method and application of enzymatic biologic salt gel

A bio-salt and gel technology, applied in instruments, analytical materials, fermentation, etc., can solve the problems of tissue rejection, high price, unsuitable for large-scale production, etc., achieve high ion conductivity, increase ion conductivity, The effect of excellent comprehensive performance

Active Publication Date: 2022-07-12
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation and purification of the ionic liquid used in the hydrated ionic liquid gel is complicated, the price is high, and it is not suitable for large-scale production.
In addition, the hydrated ionic liquid is an exogenous molecule, which is easy to cause tissue rejection and cannot be applied to implantable sensor devices.

Method used

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  • Preparation method and application of enzymatic biologic salt gel
  • Preparation method and application of enzymatic biologic salt gel
  • Preparation method and application of enzymatic biologic salt gel

Examples

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Effect test

Embodiment 1

[0057] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μl horseradish peroxidase (20 mg / ml), 10 μl acetylacetone, 50 μl glucose (40 mM) were added to 830 μl of 0.4 g / ml In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel. Its gelling time is less than 800s; its compressive strength is 1.63MPa, its compressive modulus is 46.61kPa, and it can withstand 90% of its own deformation; its tensile modulus is 46.11kPa, and its maximum tensile deformation is 2211.85%; its puncture energy is 7.21MJm -3 , can withstand 6000% of its own thickness puncture deformation; adhesion to carbon cloth is 100Nm -1 ; Can withstand the low temperature of minus 20 ℃, the ionic conductivity at r...

Embodiment 2

[0066] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μL horseradish peroxidase (20 mg / mL), 10 μL acetylacetone, 50 μL glucose (40 mM) were added to 830 μL of 0.2 g / mL In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel.

Embodiment 3

[0068] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μL horseradish peroxidase (20 mg / mL), 10 μL acetylacetone, 50 μL glucose (40 mM) were added to 830 μL of 0.6 g / mL In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel.

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Abstract

The invention relates to enzymatic bio-salt gel as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing bio-salt and water to prepare bio-salt solutions with different concentrations; (2) modifying chondroitin sulfate to prepare propenylation modified chondroitin sulfate with double bonds as a cross-linking agent; and (3) mixing the cross-linking agents prepared in the step (1) and the step (2), adding a monomer and a cascade enzyme initiation system to prepare a uniform precursor solution, and gelling the obtained gel precursor solution to prepare the bio-salt gel. Compared with the prior art, the method has the advantages that the preparation process is mild and efficient, the materials are green and environment-friendly, and the prepared bio-salt gel has enzymatic electron transfer and ion transfer capabilities and can be used for glucose sensing.

Description

technical field [0001] The invention relates to the technical field of gel materials, in particular to an enzymatic biological salt gel and a preparation method and application thereof. Background technique [0002] Bioelectronics is a rapidly developing and popular interdisciplinary subject, and its research purpose is to identify, transmit and convert biological signals into electronic signals that can be recognized by artificial machines. In recent years, wearable bioelectronic devices have played an important role in human health detection, diagnosis and treatment, and protection. For example, it can be applied to human motion sensing, biomolecular detection, skin tissue protection, flexible self-energy, minimally invasive glucose monitoring and other fields. Especially in the field of glucose detection, the global prevalence of diabetes and impaired glucose tolerance has increased significantly in recent decades, so research on glucose sensors that can continuously det...

Claims

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Application Information

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IPC IPC(8): C08F251/00C08F283/06C08F220/56C08F220/58C08F222/38C08F2/06C12P19/26G01N27/327
CPCC08F251/00C08F220/56C08F220/58C08F283/065C08F2/06C12P19/26G01N27/3271C08F222/385Y02A50/30
Inventor 丁凤霞尚英辉王启刚
Owner TONGJI UNIV
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