Anti-inflammatory and antioxidant pharmaceutical composition as well as preparation method and application thereof

A drug compound and composition technology, applied in the field of biomedicine, can solve the problems of inability to achieve precise controlled release and synergistic treatment at the same time, single therapeutic effect, loss of biological activity, etc., achieve good anti-inflammatory effect in vivo, enhance enrichment , the effect of good stability

Active Publication Date: 2022-07-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the therapeutic effect of these delivery methods is relatively single, and it is impossible to achieve precise controlled release and synergistic treatment at the same time, and the drug is volatile in the body circulation and loses its biological activity. These problems limit the practical application of the above-mentioned nano-drug delivery system

Method used

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  • Anti-inflammatory and antioxidant pharmaceutical composition as well as preparation method and application thereof
  • Anti-inflammatory and antioxidant pharmaceutical composition as well as preparation method and application thereof
  • Anti-inflammatory and antioxidant pharmaceutical composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This embodiment relates to the preparation of an anti-inflammatory and antioxidant pharmaceutical composition, wherein the polyphenolic substance is epigallocatechin gallate (EGCG), and the protein drug is bovine serum albumin (BSA). The process is as follows:

[0046] (1) Preparation of nano-drug carrier: Dissolve hyaluronic acid (HA, 0.4 g, 0.01 mmol) in deionized water (50 mL), add EDC / NHS mixed solution (20 mL, 5 mmol) to it, at room temperature After stirring the reaction for 1 h, a solution of 3-aminophenylboronic acid (APBA, 20 mL, v / v=1 / 1) dissolved in ethanol was added dropwise to the above solution and the reaction was stirred at room temperature for 24 h. The obtained solution was dialyzed in deionized water for 3 days (MWCO=3.5kDa), and after freeze-drying, a white solid product was obtained as a nano-drug carrier; the product was characterized by NMR, and the characterization results were as follows: figure 1 shown.

[0047] (2) Preparation of drug comple...

Embodiment 2

[0050] This embodiment relates to the preparation of an anti-inflammatory and antioxidant pharmaceutical composition, wherein the polyphenols are epigallocatechin gallate (EGCG), and the protein drugs are BSA and recombinant mouse interleukin 22 (IL-22). ), and the specific preparation process is as follows:

[0051] (1) Preparation of nano-drug carrier: Dissolve hyaluronic acid (HA, 0.4 g, 0.01 mmol) in deionized water (50 mL), add EDC / NHS mixed solution (20 mL, 5 mmol) to it, at room temperature After stirring the reaction for 1 h, a solution of 3-aminophenylboronic acid (APBA, 20 mL, v / v=1 / 1) dissolved in ethanol was added dropwise to the above solution and the reaction was stirred at room temperature for 24 h. The obtained solution was dialyzed in deionized water for 3 days (MWCO=3.5kDa), and after freeze-drying, a white solid product was obtained as a nano-drug carrier.

[0052] (2) Preparation of drug complex: BSA (4 mg / mL) and IL-22 (1 μg / ml) were dissolved in ultrapur...

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Abstract

The invention discloses an anti-inflammatory and anti-oxidation pharmaceutical composition and a preparation method and application thereof.The pharmaceutical composition is composed of a nano-drug carrier and a drug compound entrapped by the nano-drug carrier, and the drug compound is obtained by non-covalent bond polymerization of polyphenols and protein drugs, the nano-drug carrier is hyaluronic acid modified by 3-aminophenylboronic acid. According to the pharmaceutical composition designed by the invention, the activity of a functional protein drug is protected through the nano-drug carrier, inflammatory tissues are recognized in a targeted manner, high-concentration active oxygen at an inflammatory part is responded, and protein drugs and polyphenol substances are released, so that accurate and efficient drug administration at an injured part can be realized, and meanwhile, anti-inflammatory and anti-oxidation synergistic curative effects are exerted; and the compound has good biological safety, and has a good application prospect in the aspect of preparing anti-inflammatory and anti-oxidation drugs.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an anti-inflammatory and antioxidative pharmaceutical composition and a preparation method and application thereof. Background technique [0002] Inflammation is the basic pathological process of many diseases, and is the defensive response of the body for self-protection, including the penetration of microorganisms into tissues, the regulation between cytokines and immune signaling molecules, the removal of microorganisms and apoptotic cells, and the damage. tissue healing and other processes. If the inflammatory process cannot be interrupted and aided in repair in a timely manner, inflammation can cause damage to the body. Therefore, strategies that can effectively treat inflammatory injury have been widely studied in long-term inflammatory diseases such as arthritis and inflammatory bowel disease, and short-term inflammatory diseases such as acute pancreatitis and pneumonia. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/51A61K47/60A61K45/06A61K38/20A61K31/353A61K31/7024A61K31/352A61P29/00A61P39/06
CPCA61K45/06A61K9/19A61K47/60A61P29/00A61P39/06A61K31/352A61K31/7024A61K31/353A61K38/2026A61K38/2066A61K38/2086A61K38/20A61K9/5161A61K2300/00
Inventor 殷黎晨韦源松林娟慧胡端敏周春华
Owner SUZHOU UNIV
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