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Application of lactobacillus plantarum ST-III in preparation of product for treating autism

A technology of ST-III, Lactobacillus plantarum, applied in the field of microorganisms, to achieve the effect of reducing repetitive stereotypes, changing composition, and improving social barriers

Pending Publication Date: 2022-07-15
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on whether Lactobacillus plantarum ST-III has an ameliorating effect on autism-like behaviors in higher animals

Method used

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  • Application of lactobacillus plantarum ST-III in preparation of product for treating autism
  • Application of lactobacillus plantarum ST-III in preparation of product for treating autism
  • Application of lactobacillus plantarum ST-III in preparation of product for treating autism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Preparation of Lactobacillus plantarum ST-III powder and its viable count:

[0061] Lactobacillus plantarum ST-III (CGMCC No. 0847) in MRS liquid medium ( Merck, Germany, product number: 1106610500), after culturing at 37°C for 18-24 hours, the cells were enriched, a freeze-drying protective agent was added, and the water was removed by freeze-drying to obtain Lactobacillus plantarum ST-III bacteria powder, ST-III The viable count was 3×10 11 CFU / g.

[0062] When intervening animals, the above-mentioned Lactobacillus plantarum ST-III freeze-dried bacteria powder was suspended in 10mM PBS (pH7.2-7.4), and the viable bacteria concentration was 5×10 8 CFU / mL.

Embodiment 2

[0064] Establishment of autism model mice:

[0065] Mice adaptation: Healthy SPF grade ICR mice of reproductive age were raised in a standard animal room, with a constant temperature of 23°C, relative humidity of 50%-60%, and 12h / 12h day / night alternating light. After the mice were acclimated for 1 week, the male and female mice (female: male = 2:1) were co-caged overnight at 17:00 pm, and the female mice were checked for the presence of vaginal plugs at 9:00 am the next day.

[0066]TCS modeling: TCS was dissolved in fresh corn oil, and the drug concentration was 50 mg / ml. The successfully conceived female mice were randomly divided into 2 groups. From the 7th day of pregnancy to the 21st day of weaning, they were given TCS 50.0 mg / kg or corn oil 10 mL / kg by gavage, and named as TCS group and TCS-control group. Behavioral testing of offspring mice was performed within 6 to 8 weeks after birth. After comparing with the TCS-control group, the mice with obvious autism-like be...

Embodiment 3

[0068] Behavioral tests of TCS-ASD model mice:

[0069] Open field experiment: The test mice were placed into an open field of 50 × 50 × 40 cm and allowed to explore freely for 10 min to adapt to the environment. Immediately, the camera system (1 / 3 "SONY Super HAD CCD) was turned on to record the mouse's total movement distance, central area residence time and grooming behavior in the next 10 min, and subsequent analysis was performed.

[0070] Three-box social experiment: Divide a 60 × 40 × 22 cm plexiglass box into three interconnected chambers (A, B, C). Mice were first acclimated in an empty field for 10 minutes and then confined to room B (central room). A matched stranger mouse of the same genotype, age, sex, and treatment was placed in room C, while a small object was placed in room A. During the next 10 minutes, the test mice were allowed to move freely in the three chambers, and the overhead camera (1 / 3 "SONY SuperHAD CCD) recorded the time of the test mice's olfact...

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Abstract

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus plantarum ST-III in preparation of a product for treating autism, and the preservation number of the lactobacillus plantarum ST-III is CGMCC No.0847. After the lactobacillus plantarum ST-III intervenes for two weeks, repeated engraving and anxiety behaviors can be reduced, the social contact time proportion can be increased, and social contact obstacles can be improved; and the composition of intestinal flora can be changed, so that the structure of the intestinal flora related to the autism is remarkably improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the use of Lactobacillus plantarum ST-III in the preparation of products for treating autism. Background technique [0002] Autism spectrum disorder (Autism Spectrum Disorder, ASD) is a serious neurodevelopmental disorder characterized by communication impairment and social interaction impairment, narrow interests and repetitive stereotyped movements. It has been reported that the prevalence of ASD continues to increase globally, with male children 4-5 times the incidence of female children. ASD is currently considered to be the result of the interaction of genetic factors and environmental risk factors. Prenatal or postnatal exposure to chemicals and drugs, air pollution, stress, intrauterine infection and dietary factors can increase the risk of ASD. Regrettably, however, there is currently no cure for ASD, which brings a heavy burden to society and families. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P25/00A23L33/135
CPCA61K35/747A61P25/00A23L33/135A23V2002/00A23V2400/169A23V2200/30A23V2200/3204
Inventor 刘振民游春苹张怡琳李瑞盈张红发楚阳阳
Owner BRIGHT DAIRY & FOOD
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