MNP marker site of herpes simplex virus, primer composition, kit and application of MNP marker site

Abstract

The invention discloses MNP marker sites of herpes simplex virus, a primer composition, a kit and application of the MNP marker sites, the primer composition and the kit, and the MNP marker sites refer to genome regions which are screened from herpes simplex virus type I and type 2 genomes, are distinguished from other species and have a plurality of nucleotide polymorphisms in subtypes, and comprise marker sites of MNP-1 to MNP-14; the primers are as shown in SEQ ID NO. 1 to SEQ ID NO. 28. The MNP marker site can be used for specifically identifying and typing the herpes simplex virus; the primer does not interfere with one another, integrates multiple amplification and sequencing technologies, can perform sequence analysis on all marker sites of multiple samples at one time, has the detection advantages of high throughput, multiple target points, high sensitivity, high precision and variation monitoring, can be applied to herpes simplex virus identification and genetic variation detection of large-scale samples, and has wide application prospects. The method has important significance on scientific research and epidemic prevention monitoring of the herpes simplex virus.

Description

technical field [0001] The embodiments of the present invention relate to the field of biotechnology, and in particular, to an MNP marker site of herpes simplex virus, a primer composition, a kit and applications thereof. Background technique [0002] Herpes Simplex Virus (HSV) is a DNA virus that can cause a variety of diseases, such as gingivostomatitis, keratoconjunctivitis, encephalitis, as well as reproductive system infections and neonatal infection. Pregnant women are infected with HSV-I, and the virus may infect the fetus through the placenta, resulting in miscarriage, stillbirth or congenital malformations. Neonatal herpes is a common and serious clinical infection. Therefore, HSV screening is one of the must-check items for pregnant women. [0003] The existing detection and typing methods are mainly based on antigen-antibody reaction, and HSV is divided into two serotypes, HSV-1 and HSV-2. However, this detection and typing method has the limitations of low sens...

AI Technical Summary

Problems solved by technology

Based on RCA and fluorescent PCR, only one of HSV-1 and HSV-2 can be detected at a time, or both can be detected at the same time, but the number of markers detected is limited, usually only one marker is detected for one type, and mutations cannot be detected, and a few The detection of one site is easy to fail, and has the limitations of low detection efficiency and low accuracy

Methods based on sequencing technology, such as metagenomic sequencing technology, often include a large amount of host sequencing data, especially when detecting low-concentration HSV samples, ultra-deep sequencing is required, and it is extremely difficult to detect mutations for typing. Accepted Sequencing Costs

Existing methods mainly distinguish subtypes by detecting SNP markers, but SNP markers have the limitations of low polymorphism, high typing error rate and weak species discrimination ability

Herpes simplex virus is a group organism. The variation of individuals in the group leads to the existence of multiple allele types and low-frequency genotypes at one marker site. SNP markers are dimorphic markers, and it is difficult to capture the diversity of common organisms Allelic type, there are defects of low marker polymorphism, low detection method accuracy and sensitivity

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