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Preparation device and preparation method of solid-state fermentation enzyme preparation

A solid-state fermentation and preparation device technology, applied in biochemical equipment and methods, solid-phase fermentation bioreactors, enzymology/microbiology devices, etc., can solve the problem of poor stirring effect and heating effect, and uneven heating of the fermentation liquid To avoid uneven heating, improve the stirring effect, and increase the contact area

Inactive Publication Date: 2022-07-29
枣庄全鼎生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation device for solid-state fermentation enzyme preparations, to solve the problem that the stirring effect and heating effect in the above-mentioned background technology are not good, and it is easy to cause uneven heating of each part of the fermentation liquid.

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  • Preparation device and preparation method of solid-state fermentation enzyme preparation
  • Preparation device and preparation method of solid-state fermentation enzyme preparation
  • Preparation device and preparation method of solid-state fermentation enzyme preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A preparation method of a biologically fermented high-efficiency amino acid type organic enzyme preparation applied to a preparation device of a solid-state fermentation enzyme preparation, comprising the following steps:

[0047] S1: Mix 30% of kelp, 20% of leaves, 20% of hay, and 30% of fish meal in proportion, smash it, and sterilize it. The moisture content is 40% to obtain fermented raw materials, which are poured into a fermented pot for later use;

[0048] S2: prepare enzyme bacteria liquid;

[0049] S3: prepare EM bacterial solution;

[0050] S4: For one fermentation, transfer the enzyme bacteria liquid prepared in step S2 into the tank 1, turn on the heating tube, adjust the temperature to 38°C, and start the driving motor and driving components, the speed of the driving motor is 150r / min, and the air inlet is turned on. Air valve, let in sterile air, adjust pH to 7.2 with acidity regulator or alkalinity regulator, and ferment aerobic for 48 hours. During the ...

Embodiment 2

[0054] A method for preparing a deodorant enzyme preparation by fermentation applied to a preparation device of a solid-state fermentation enzyme preparation, comprising the following steps

[0055] S1 Liquid Seed Preparation

[0056] The aqueous solution composition of the seed medium is (g / L): starch 20; (NH 4 ) 2 HPO 4 6;K 2 HPO 4 0.9; MgSO 4 0.8; CaCl 2 0.8; corn steep liquor 1.5.

[0057] Preparation method: add water and the above various raw materials weighed according to the above solubility into the batching container, stir and heat until each component is dissolved, adjust the pH value to 7.0 with NaOH solution, put it into a seed tank, and under the condition of steam pressure of 1Kg / cm2 Under sterilization for 30min, cooling to cool the medium to 32 ℃, to obtain sterilized seed medium for use;

[0058] Liquid seed preparation: Inoculate 1 bottle (50ml) of Streptomyces sp. DS-021 spore suspension of eggplant bottle with a seed age of 5 days per 100L steriliz...

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Abstract

The present invention discloses a solid state fermentation enzyme preparation preparation device, and relates to the technical field of solid state fermentation enzyme preparation preparation, the solid state fermentation enzyme preparation preparation device comprises a fermentation tank, the top of the fermentation tank is provided with a feed inlet, the feed inlet is provided with a cover body, the bottom of the fermentation tank is provided with a liquid outlet pipe, and the liquid outlet pipe is provided with a valve; the isolating mechanism and the stirring mechanism are arranged in the tank body, and the isolating mechanism comprises an annular partition plate fixed on the inner side wall of the tank body and a cylindrical partition plate connected to the inner side of the annular partition plate and formed by extending downwards; a stirring cavity is formed in the inner side of the cylindrical partition plate, and an annular cavity is formed between the cylindrical partition plate and the inner side wall of the tank body. According to the device, fermentation liquor continuously passes through the annular cavity and then is discharged from the liquid discharge hole, and in the whole process, the fermentation liquor forms a flowing state, so that the contact area between the fermentation liquor and the heating pipe is increased, the stirring effect is improved, the fermentation liquor is integrally and uniformly heated, and the situation that all parts of the fermentation liquor are heated unevenly is avoided.

Description

technical field [0001] The invention relates to the technical field of preparation of solid-state fermentation enzyme preparations, in particular to a preparation device of solid-state fermentation enzyme preparations and a preparation method thereof. Background technique [0002] Currently, enzyme preparations usually use two production processes, solid state fermentation and liquid fermentation. Among them, solid-state fermentation is a fermentation method of microorganisms on a solid substrate without or substantially free of free water. The gas, liquid and solid three phases coexist in the solid substrate, that is, the porous solid substrate contains water and water-insoluble substances coexist. Compared with liquid fermentation, solid-state fermentation has the following characteristics: there is no free water flow in the solid-state fermentation medium, which is suitable for the growth of microorganisms with a water activity of 0.93 to 0.98, which limits the scope of a...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/02C12M1/16C12M1/00B01F27/90B01F35/30B01F35/90C12N9/00
CPCC12M21/14C12M21/16C12M27/02C12M41/18C12N9/00
Inventor 任全亮朱政张良任全明韩雪侠梁亚任绪志任俊超
Owner 枣庄全鼎生物科技股份有限公司