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Microbial agent and preparation method thereof

A technology of microorganisms and bacteriostatic agents, applied in the field of microorganisms, can solve the problems of large amount of fermentation broth and inconvenient practical application, and achieve the effect of increasing plant height and promoting the growth of wheat seedlings.

Active Publication Date: 2022-08-05
YANTAI HONGYUAN BIOLOGICAL FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of fermentation liquid is relatively large, and it is inconvenient for practical application.

Method used

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  • Microbial agent and preparation method thereof
  • Microbial agent and preparation method thereof
  • Microbial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 A kind of Bacillus velesi

[0063]The bacterial strain of the present invention is obtained by screening other products in the plate confrontation experiment of grape white rot pathogenic bacteria, and the number is YTHY20220301. After isolation and purification, it was determined to be Bacillus velesi by sequencing by Qingdao Peisenuo Gene Biotechnology Co., Ltd. And it was deposited in the General Microbiology Center of China Microorganism Culture Collection Management Committee on April 18, 2022, and the preservation number is CGMCC No. 24706.

[0064] Nutrient agar medium can be used for the cultivation of this strain: peptone 10.0g, beef extract 3.0g, sodium chloride (NaCl) 5.0g, agar 18.0g, distilled water 1000mL, pH 7.2-7.5.

[0065] The specific fermentation conditions are: 37°C, 20-24h, pH7.3, aeration, 120m 3 / h-180m 3 / h.

[0066] Fermentation step: inoculate the strain in a 5L fermenter to prepare a seed solution, transfer the seed solution ...

Embodiment 2

[0069] Example 2 Changes and bacteriostatic ability of Bacillus velesi at different temperatures

[0070] In order to verify the effect of different temperatures on the effective viable count of Velez, routine storage experiments were designed under the conditions of 15°C (room temperature), 38°C and 54°C. In addition, the high temperature test of water bath treatment at 70°C for 5h and high temperature and high pressure at 121°C for 20min was added. The number of effective viable bacteria was determined by plate coating, and the antibacterial ability was verified by plate confrontation.

[0071] (1) Storage at different temperatures

[0072] Divide the Bacillus velesi bacteria solution and store at 15°C (room temperature), 38°C and 54°C respectively.

[0073] see the results figure 1 , 38°C for the 54th day (top) and 54°C for the 2nd day (bottom).

[0074] Velez stored at 15℃ has slow stratification and no flatulence;

[0075] Velez stored at 38°C has a slow delaminatio...

Embodiment 3

[0091] Example 3 The effect of Bacillus velesi seed soaking on the emergence of wheat

[0092] Pick full-grained wheat seeds, first sterilize them with 10% sodium hypochlorite for 10 minutes, rinse with distilled water 3 times, then soak the wheat seeds with different concentrations of Belles overnight, rinse with distilled water 3 times, and place the seeds neatly on a 2-layer filter paper. In a petri dish (add 5 mL of distilled water), 30 seeds per dish, each treatment was repeated 3 times, placed in an artificial climate box, the temperature was 25 °C, the humidity was 70%, the light-dark cycle was 12h / 12h, and the germination and growth were observed. .

[0093] The concentration of Bacillus velesi bacteria solution is: CK (clean water), T0 (stock solution), T1 (diluted 500 times), T2 (1000 times), T3 (3000 times), T4 (5000 times).

[0094] (1) The effect of different concentrations of Bacillus velesi bacteria solution on seed germination

[0095] Depend on Figure 5 It...

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Abstract

The invention relates to the field of microorganisms, and particularly provides bacillus velezensis, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.24706, is preserved in China General Microbiological Culture Collection Center on April 18, 2022, has a preservation address of Beijing, China, and is classified and named as bacillus velezensis. The bacillus velezensis provided by the invention can promote the growth of crops, has a better antibacterial effect, and has a better application prospect in the fields of fertilizers and pesticides.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a microbial inoculant for promoting crop growth and inhibiting pathogenic bacteria and a preparation method thereof. Background technique [0002] Bacillus velesi belongs to Bacillus, which is widely distributed in nature. The bacteria grow fast, have strong stress resistance, and are rich in metabolites. At the same time, the bacteria play an important role in biological control, drug research and development, food fermentation and industrial applications. [0003] The prior art discloses a variety of isolated strains of Bacillus velesi, whose functions are mainly to promote plant growth and inhibit crop pathogens. It can prevent and control diseases of various crops, such as poplar canker, wheat scab, cucumber fusarium wilt, bitter gourd fusarium wilt, tomato early blight, tobacco scab and so on. [0004] The Chinese invention patent with the application number of CN...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/60A01N63/22A01P21/00A01P3/00C12R1/07
CPCC12N1/205C12N1/20C05G3/00C05G3/60A01N63/22A01P21/00A01P3/00C12R2001/07Y02A40/10
Inventor 王萌吴春峰孙明宾吴建燕隋晓琳于军
Owner YANTAI HONGYUAN BIOLOGICAL FERTILIZER CO LTD
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