Starch-based nanoparticles of double-embedded beta-carotene as well as preparation method and application of starch-based nanoparticles

A carotene and nanoparticle technology, applied in the field of emulsification, can solve problems such as protein instability, easy aggregation, and unsatisfactory emulsification performance of a single protein, and achieve the effects of wide sources, simple preparation process, and high stability against environmental stress

Active Publication Date: 2022-08-09
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, proteins are unstable and readily aggregate under environmental stresses such as pH, heat, and ionic strength
On the other hand, the emulsifying properties of individual proteins are not ideal

Method used

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  • Starch-based nanoparticles of double-embedded beta-carotene as well as preparation method and application of starch-based nanoparticles
  • Starch-based nanoparticles of double-embedded beta-carotene as well as preparation method and application of starch-based nanoparticles
  • Starch-based nanoparticles of double-embedded beta-carotene as well as preparation method and application of starch-based nanoparticles

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preparation example Construction

[0023] Specifically, as an aspect of the technical solution of the present invention, a method for preparing starch-based nanoparticles with double-embedded β-carotene involved includes:

[0024] The starch is subjected to enzymatic hydrolysis treatment, precipitation and classification treatment to obtain dextrin;

[0025] Dextrin is acylated with butyric anhydride to obtain butyrylated dextrin;

[0026] By adopting a co-precipitation method, the butyrylated dextrin and β-carotene are embedded to obtain a butyrylated dextrin / β-carotene inclusion complex;

[0027] Ultrasonic modification of gliadin is carried out to obtain ultrasonically modified gliadin;

[0028] The pH value of the alkaline mixed system comprising the butyrylated dextrin / β-carotene inclusion complex, ultrasonically modified gliadin and β-carotene is adjusted to 7.5 and self-assembly occurs to prepare a double The starch-based nanoparticles embedded with beta-carotene, wherein the pH value of the alkaline m...

Embodiment 1

[0069] (1) Preparation of corn dextrin

[0070] Using 0.01 mol / L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer with pH 5, corn starch was prepared into a starch suspension with a mass fraction of 5%, and then placed in a boiling water bath and stirred for 1 h until the starch was completely gelatinized. After the gelatinized starch is cooled to 45°C, pullulanase is added for hydrolysis reaction, the time is 22h, the pH value of the hydrolysis reaction system is 5, and the addition amount of pullulanase is 100U / g (calculated on the dry basis of starch); After the hydrolysis treatment was completed, the product was centrifuged (4500 g, 20 min), and the supernatant was rotary-evaporated at 55° C. and lyophilized to obtain crude dextrin.

[0071] The crude dextrin suspension with a concentration of 5% was prepared using an ethanol solution with a volume ratio of ethanol and deionized water of 1:1, stirred in a water bath at 60 °C for 4 h, and then centrifuged at 4...

Embodiment 2

[0084] (1) Preparation of corn dextrin

[0085] Using 0.01 mol / L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer with pH 5.5, corn starch was prepared into a starch suspension with a mass fraction of 5%, and then placed in a boiling water bath and stirred for 1 h until the starch was completely gelatinized. After the gelatinized starch is cooled to 50°C, pullulanase is added for hydrolysis reaction, the time is 23h, the pH value of the hydrolysis reaction system is 5.5, and the addition amount of pullulanase is 125U / g (calculated on the dry basis of starch); After the hydrolysis treatment was completed, the product was centrifuged (4500 g, 20 min), and the supernatant was rotary-evaporated at 55° C. and lyophilized to obtain crude dextrin.

[0086] The crude dextrin suspension with a concentration of 5% was prepared by using an ethanol solution with a volume ratio of ethanol and deionized water of 1:1, stirred in a water bath at 60 °C for 4 h, and then centrifuged...

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Abstract

The invention discloses double-embedded beta-carotene starch-based nano-particles as well as a preparation method and application of the double-embedded beta-carotene starch-based nano-particles. The preparation method comprises the following steps: carrying out enzymolysis treatment and precipitation grading treatment on starch to prepare dextrin; butyric anhydride is adopted to carry out acylation treatment on dextrin, and butyrylated dextrin is prepared; carrying out embedding treatment on the butyrylated dextrin and beta-carotene by adopting a coprecipitation method, so as to prepare a butyrylated dextrin / beta-carotene inclusion compound; the gliadin is subjected to ultrasonic modification treatment, and ultrasonic modified gliadin is prepared; enabling an alkaline mixed system containing the butyrylated dextrin / beta-carotene inclusion compound, the ultrasonic modified gliadin and the beta-carotene to be subjected to self-assembly, so as to prepare the starch-based nanoparticles with the double embedded beta-carotene. The double-embedded starch-based nanoparticles prepared by the method have relatively high inclusion rate and high environmental stress resistance stability, and have wide application prospects in the field of bioactive substances or emulsions.

Description

technical field [0001] The invention belongs to the technical field of emulsification, and in particular relates to a double-embedded β-carotene starch-based nanoparticle and a preparation method and application thereof. Background technique [0002] Starch, as a polysaccharide, is one of the most abundant renewable biopolymers on earth, and has received extensive attention due to its important advantages such as biocompatibility, biodegradability, cheapness and ease of modification. Dextrin is a kind of starch derivative, which is obtained by debranching of starch, and its molecular weight is lower than that of starch. Acylated dextrin, one of the dextrin derivatives, is an amphiphilic biopolymer that is easy to synthesize, non-toxic and has good biocompatibility. At the same time, the hydrophobic cavity of dextrin can interact with the hydrophobic hydrocarbon chain of β-carotene to encapsulate β-carotene to form inclusion complexes. [0003] Polysaccharides and proteins ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L29/30A23L29/00A23L33/105A23P10/30
CPCA23L29/35A23L29/045A23L33/105A23P10/30A23V2002/00A23V2200/30A23V2250/211A23V2250/5114A23V2250/5482
Inventor 章宝陈品王如梦何廷石刘欣月
Owner HEFEI UNIV OF TECH
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