Orally applied medicine containing Nostoc which expressing human tumor necrosin factor alpha

A technology of tumor necrosis factor and oral preparations, applied in the field of human tumor necrosis factor alpha oral preparations, to achieve the effect of simplifying downstream processes and facilitating absorption

Inactive Publication Date: 2004-10-13
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the severity of systemic toxicity limits its application

Method used

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  • Orally applied medicine containing Nostoc which expressing human tumor necrosin factor alpha

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Experimental program
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Embodiment

[0019] 1. Bacterial Strains and Vector Plasmids 1. Cyanobacteria species: Nostoc muscorum, provided by Institut Pasteur, France. 2. Escherichia coli strain: E. coli HB101 was used as the recipient strain of the transformed plasmid. 3. Plasmid:

[0020] (1) pRL-TC was constructed by our laboratory, and rhTNF was inserted into the multiple cloning site of plasmid pRL439. pRL439 carries psbA promoter, ampicillin resistance gene, and non-damaged bom region (necessary region for transfer) (2) pDC-8. (3) RP4+pRL542 in E.coli HB101 is a helper plasmid.

[0021] Second, the cultivation of cyanobacteria

[0022] Cyanobacteria are generally cultured in liquid medium, placed in a Gallenkamp-type light-cooled rotary incubator for shaking culture, rotating speed 130rpm, 25-30°C, changing the culture medium every 10 days, and inoculating at a ratio of 1:50 for passage.

[0023] When purifying algae species, the methods of streak separation and coating separation can be used. On a solid...

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Abstract

An orally applied medicine for treating the tumor in digestive tract contains the Nostocales where human tumor necrosin alpha (hTNF alpha) is expressed. It features low toxin.

Description

(1) Technical field [0001] The present invention relates to an oral agent, specifically, the present invention relates to an oral agent of human tumor necrosis factor alpha. (2) Background technology [0002] Tumor Necrosis Factor α (TNFα for short) is a multifunctional cytokine produced by activated monocyte-macrophages. Human active TNFα is composed of three non-glycoprotein monomers with a molecular weight of 17KDa, which form a sheet-folded structure, and a non-covalent composition of a disulfide bond between Cys69-Cys101 is passed between them. TNFα derived from different species of mammals has extremely high homology in primary structure, and there is no obvious species-specificity in biological activity. The isoelectric point of purified TNFα is around 5.3; stable at pH 6-8; sensitive to acid: sensitive to proteolytic enzymes (trypsin, chymotrypsin, etc.); 50% saturated ammonium sulfate can make it precipitate. It is inactivated at room temperature when it is repeat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P35/00C12N15/28
Inventor 施定基刘凤龙邵宁彭国宏林晨冉亮
Owner INST OF BOTANY CHINESE ACAD OF SCI
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