Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Orally applied medicine containing Nostoc which expressing human tumor necrosin factor alpha

A technology of tumor necrosis factor and oral preparations, applied in the field of human tumor necrosis factor alpha oral preparations, to achieve the effect of simplifying downstream processes and facilitating absorption

Inactive Publication Date: 2004-10-13
INST OF BOTANY CHINESE ACAD OF SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the severity of systemic toxicity limits its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Orally applied medicine containing Nostoc which expressing human tumor necrosin factor alpha

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1. Bacterial Strains and Vector Plasmids 1. Cyanobacteria species: Nostoc muscorum, provided by Institut Pasteur, France. 2. Escherichia coli strain: E. coli HB101 was used as the recipient strain of the transformed plasmid. 3. Plasmid:

[0020] (1) pRL-TC was constructed by our laboratory, and rhTNF was inserted into the multiple cloning site of plasmid pRL439. pRL439 carries psbA promoter, ampicillin resistance gene, and non-damaged bom region (necessary region for transfer) (2) pDC-8. (3) RP4+pRL542 in E.coli HB101 is a helper plasmid.

[0021] Second, the cultivation of cyanobacteria

[0022] Cyanobacteria are generally cultured in liquid medium, placed in a Gallenkamp-type light-cooled rotary incubator for shaking culture, rotating speed 130rpm, 25-30°C, changing the culture medium every 10 days, and inoculating at a ratio of 1:50 for passage.

[0023] When purifying algae species, the methods of streak separation and coating separation can be used. On a solid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An orally applied medicine for treating the tumor in digestive tract contains the Nostocales where human tumor necrosin alpha (hTNF alpha) is expressed. It features low toxin.

Description

(1) Technical field [0001] The present invention relates to an oral agent, specifically, the present invention relates to an oral agent of human tumor necrosis factor alpha. (2) Background technology [0002] Tumor Necrosis Factor α (TNFα for short) is a multifunctional cytokine produced by activated monocyte-macrophages. Human active TNFα is composed of three non-glycoprotein monomers with a molecular weight of 17KDa, which form a sheet-folded structure, and a non-covalent composition of a disulfide bond between Cys69-Cys101 is passed between them. TNFα derived from different species of mammals has extremely high homology in primary structure, and there is no obvious species-specificity in biological activity. The isoelectric point of purified TNFα is around 5.3; stable at pH 6-8; sensitive to acid: sensitive to proteolytic enzymes (trypsin, chymotrypsin, etc.); 50% saturated ammonium sulfate can make it precipitate. It is inactivated at room temperature when it is repeat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P35/00C12N15/28
Inventor 施定基刘凤龙邵宁彭国宏林晨冉亮
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com