Process for preparing ginkgo leaf extract powder injection

A technology of ginkgo biloba leaves and extract, applied in the field of traditional Chinese medicine preparation, can solve problems such as unreasonable process route, low total amount of active ingredients, and lack of industrialization conditions, and achieves reasonable extraction process route, stable and feasible preparation process, and production cost. low effect

Inactive Publication Date: 2006-10-25
AOLING BODA MEDICINE SCI & TECH DEV BEIJING
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the existing technology: the total amount of active ingredients extracted is not high, the process route is not reasonable enough, or the industrialization conditions are not available, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The preparation of the ginkgo leaf extract of the present invention uses the ginkgo leaves as raw materials. Ginkgo biloba leaves are picked in autumn when they are still green, removed from impurities, and dried in the sun. The selected ginkgo leaves contain more than 0.85% of total flavonol glycosides and more than 0.25% of total terpene lactones.

[0021] The preparation method is as follows:

[0022] 1. Extraction: crush the dried ginkgo leaves to 10-20 mesh, extract several times with 65-85% ethanol, combine the extracts, filter, recover ethanol, and concentrate to a thick paste.

[0023] The influence of ginkgo leaf fineness on the extraction effect is that too fine crushing will not only increase impurities, but also affect the proposal of total flavonol glycosides and total terpene lactones. It is more suitable to use 10-20 mesh broken leaves for extraction. The particle size range of ginkgo leaves determined by the present invention is It is 10-20 mesh.

[0...

Embodiment 1

[0056] Embodiment 1: the preparation of Ginkgo biloba extract

[0057] Crush the dried ginkgo leaves of test amount to below 10-20 mesh, extract three times with 75% ethanol, add alcohol 12 times for the first time, soak for 1.0 hour, reflux extraction for 1.0 hour, add 8,6 Double the amount, reflux extraction for 0.5 hours, combine the extracts, filter, recover ethanol under reduced pressure, concentrate to a thick paste, add an appropriate amount of 20% ethanol, stir well, reflux extraction for 0.5 hours, filter, and process the precipitation again in the same way , combine the filtrate, stir and add 60ml of 1% chitosan solution to flocculate, let it stand, centrifuge, recover the ethanol from the supernatant until it has no alcohol smell, add water to an appropriate amount, load the sample on the treated polyamide column, and elute with 10% ethanol , collected and eluted to a set amount, then eluted the polyamide column with 75% ethanol, collected the eluate to a set amount...

Embodiment 2

[0058] Embodiment 2: Preparation of ginkgo leaf extract powder injection

[0059] Take ginkgo biloba extract, add absolute ethanol, stir until dissolved, dilute with about 2500ml of water for injection at 60-90°C, stir, add mannitol, stir to dissolve, adjust the pH value to about 4.8 with 1mol / L NaOH, add activated carbon ( 0.03% g / v), keep warm, stir for 10 minutes, decarbonize and filter, add water for injection to 3000ml. As a semi-finished solution.

[0060] Take the semi-finished solution, measure the pH value, content of lactone and flavone. Filtrate twice, the first time using ordinary filter paper to filter out carbon and other substances, and the second time using a microporous filter membrane with a pore size of 0.2 μm to filter under pressure. According to the test results of the semi-finished product, the solution is subpackaged under a sterile environment (local level 100). Make 3ml / tube, put it in a freeze dryer, adjust the temperature of the freezer to -30°C,...

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Abstract

The invention relates to a preparation method of ginkgo leaf extract powder injection, which belongs to the technical field of traditional Chinese medicine preparation. The total content of total flavones and total terpene lactones in the ginkgo leaf extract reaches more than 80%, wherein the total flavonoids reach more than 70%, the flavonol glycosides reach more than 40%, and the total terpene lactones content reaches more than 10%. The preparation method of the ginkgo leaf extract powder injection of the present invention comprises the steps of liquid preparation, filtration, packaging, freeze-drying and the like. The extraction process route of the ginkgo leaf extract of the invention is reasonable, and the processes of extraction, impurity removal, purification, extraction, column separation and refining meet the requirements of large-scale production and meet industrial conditions. The preparation method of the ginkgo leaf extract powder injection of the present invention shows that the preparation process is stable and feasible through stability test research, and a new dosage form of the ginkgo leaf extract is added for clinical use, which meets the clinical needs. The safety test shows that the ginkgo leaf extract powder injection of the present invention does not produce allergic reaction and hemolysis, and the intravenous injection has no irritation to blood vessels.

Description

technical field [0001] The invention relates to a preparation method of ginkgo leaf extract powder injection, in particular to a raw material medicine and a preparation for treating cardiovascular and cerebrovascular diseases, peripheral blood vessels and other blood circulation disorders, and belongs to the technical field of traditional Chinese medicine preparation. Background technique [0002] Blood circulation disorders such as cardiovascular and cerebrovascular diseases and peripheral blood vessels are one of the most common clinical diseases and a major class of diseases that affect people's health and longevity. The role of Ginkgo biloba extract and its preparations in cardiovascular and cerebrovascular diseases and peripheral vascular diseases has attracted more and more attention from all over the world. Ginkgo biloba L. is an ancient relic plant, also known as ginkgo tree. Ginkgo biloba is a rare tree species in my country and a precious medicinal plant resource ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K36/16A61K9/14A61P9/00
Inventor 庄洪波
Owner AOLING BODA MEDICINE SCI & TECH DEV BEIJING
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