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Method for culturing an artificial cultured pseudosciaena crocea

A cultivation method and artificial cultivation technology, applied in the field of cultivation of large yellow croaker, can solve the problems such as inability to effectively select the broodstock of the broiled yellow croaker, slow growth rate, low survival rate, etc., and achieve high protein composition, fast growth rate and survival rate. high rate effect

Inactive Publication Date: 2007-04-04
NINGBO UNIV +1
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  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, it has been found that the farmed large yellow croaker domesticated from the wild large yellow croaker of the Fujian-Yuedong ethnic group has some shortcomings because it has not been selected: First, the body shape is poor: the tail length / tail height is 3.2 , body length / body height is 3.4; second, the growth rate is slow; third, there are still defects in quality: its protein content is generally 16.47%, and the total amino acid content in muscle is generally 139.0mg / g, of which ten kinds of essential amino acid content It is 47.4mg / g, and the content of four flavor amino acids is 69.9mg / g, and its fat content is generally 3.14; the fourth is that the survival rate is low, only about 70%.
However, this breeding method cannot effectively select yellow croaker broodstock that can be used for breeding. Often the inspected yellow croaker broodstock has died after passing the inspection, and the yellow croaker broodstock in the same group cannot be determined to be exactly the same as the inspected broodstock. identical

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  • Method for culturing an artificial cultured pseudosciaena crocea
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  • Method for culturing an artificial cultured pseudosciaena crocea

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Embodiment 1

[0015] Embodiment 1: Gather and catch 244 wild primary large yellow croakers of the Daiqu nationality in Daiqu Yang, Zhoushan, Zhejiang. Through domestication and breeding, 11 sexually mature broodstock were bred, and 280,000 wild offspring (3cm) were bred in the autumn. After breeding, according to the shape and size of the body, more than 500 yellow croakers of the first generation of Daiqu family were selected from 3,790 tails and put into boxes to cultivate broodstock. After a period of cultivation, blood DNA templates were extracted by using the method of blood collection from the tail artery of the parents of yellow croakers. Carry out RAPD analysis and comparison, use FR-980 bioelectrophoresis image analyzer to take pictures of blood DNA molecular markers, as shown in Figure 3, select 152 parent fish with better gonad development with a specific marker of 871bp, artificially induce labor, hatch and cultivate The second generation of offspring is used for artificial breed...

Embodiment 2

[0020] Embodiment 2: Gather and catch 244 wild primary large yellow croakers of the Daiqu nationality in Daiquyang, Zhoushan, Zhejiang. Through domestication and breeding, 11 sexually mature broodstocks have been bred, and 280,000 wild seedlings (3cm) have been bred in autumn After breeding, according to the shape and size of the body, more than 500 yellow croakers of the first generation of Daiqu family were selected from 3790 tails and put into boxes to cultivate broodstock. After a period of cultivation, the clipped fin rays were used as the expression of isozymes Materials: FR-980 bioelectrophoresis image analyzer was used to take pictures of molecular markers of fin ray isozymes, as shown in Figure 4, and 152 broodstock with a malic enzyme (ME) specific marker with better gonad development were selected for Artificially induced spawning, hatching and cultivating the second generation of offspring for artificial cultivation and breeding. Its caudal peduncle length / caudal p...

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Abstract

The invention discloses an artificial cultured large yellow crocker and the method for culturing it, including: increasing the yellow crocker parental generation breeding in the culture process, applying to group breeding technique combined with molecular labeling auxiliary breeding technique, to breeding merit strain rapidly, in such a way the cultured large yellow crocker provided with good shape, fast growth rate, sound quality and high survivial rate, and the habitus parameter: the length of the caudal peduncle / the height of the caudal peduncle as 3.6, the length of the body the height of the body as 3.9, the biochemical component: 18.34 percent protein contents, 152.0 mg / g total amino acid contents in the muscle, 2.89 percent fat contents, the DNA molecular in the blood provided with 871bp specific tag, and the fin ray isoenzyme provided with a malic enzyme(ME) specific tag.

Description

technical field [0001] The invention relates to an artificially cultivated seawater product, in particular to a method for cultivating artificially cultivated large yellow croaker. Background technique [0002] The existing large yellow croaker (Pseudosciaena crocea Richardson), which belongs to the genus Pseudosciaidae and the genus Pseudosciaena, is commonly known as yellow croaker, yellow croaker, etc. It was once one of the four famous marine fishing targets in my country. The large yellow croaker in the coastal areas of China can be divided into three geographical races according to their morphological characteristics, namely, the Daiqu tribe mainly produced in Daiquyang, the Min-Yuedong tribe mainly produced in Guanjingyang and the Naozhou tribe. The Daiqu ethnic group has the most complex structure, the largest number, the largest individuals, and the best quality; followed by the Fujian-Yuedong ethnic group, and the Naozhou ethnic group has the simplest structure and...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李明云徐万土竺浚全郑根兴
Owner NINGBO UNIV
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