Method for culturing an artificial cultured pseudosciaena crocea
A cultivation method and artificial cultivation technology, applied in the field of cultivation of large yellow croaker, can solve the problems such as inability to effectively select the broodstock of the broiled yellow croaker, slow growth rate, low survival rate, etc., and achieve high protein composition, fast growth rate and survival rate. high rate effect
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Embodiment 1
[0015] Embodiment 1: Gather and catch 244 wild primary large yellow croakers of the Daiqu nationality in Daiqu Yang, Zhoushan, Zhejiang. Through domestication and breeding, 11 sexually mature broodstock were bred, and 280,000 wild offspring (3cm) were bred in the autumn. After breeding, according to the shape and size of the body, more than 500 yellow croakers of the first generation of Daiqu family were selected from 3,790 tails and put into boxes to cultivate broodstock. After a period of cultivation, blood DNA templates were extracted by using the method of blood collection from the tail artery of the parents of yellow croakers. Carry out RAPD analysis and comparison, use FR-980 bioelectrophoresis image analyzer to take pictures of blood DNA molecular markers, as shown in Figure 3, select 152 parent fish with better gonad development with a specific marker of 871bp, artificially induce labor, hatch and cultivate The second generation of offspring is used for artificial breed...
Embodiment 2
[0020] Embodiment 2: Gather and catch 244 wild primary large yellow croakers of the Daiqu nationality in Daiquyang, Zhoushan, Zhejiang. Through domestication and breeding, 11 sexually mature broodstocks have been bred, and 280,000 wild seedlings (3cm) have been bred in autumn After breeding, according to the shape and size of the body, more than 500 yellow croakers of the first generation of Daiqu family were selected from 3790 tails and put into boxes to cultivate broodstock. After a period of cultivation, the clipped fin rays were used as the expression of isozymes Materials: FR-980 bioelectrophoresis image analyzer was used to take pictures of molecular markers of fin ray isozymes, as shown in Figure 4, and 152 broodstock with a malic enzyme (ME) specific marker with better gonad development were selected for Artificially induced spawning, hatching and cultivating the second generation of offspring for artificial cultivation and breeding. Its caudal peduncle length / caudal p...
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