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A feed additive and probiotic technology, applied in application, animal feed, animal feed, etc., can solve the problems of high cost, labor and time, high price, etc., and achieve cost reduction, simple manufacturing process and high feed conversion rate. Effect

Inactive Publication Date: 2007-05-30
浙江德清利欣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a green feed additive that can promote the healthy growth of livestock and poultry and a method for producing and preparing this green feed additive, so as to solve the problem of high cost, expensive and labor-intensive production of green feed additives in the prior art. time-consuming disadvantage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Konjac flour is selected as the polycarbohydrate, Bacillus subtilis is selected as the probiotic, and peptone and beef extract are selected as the nitrogen source. It is made by using the β-mannanase produced by Bacillus subtilis itself to decompose konjac flour to produce mannose and mannooligosaccharides by fermentation. The final product contains mannose, manno-oligosaccharides, β-mannanase and Bacillus subtilis. The konjac flour used in this embodiment is konjac flour containing more than 80% β-glucomannan, wherein the content of starch accounts for 2.4%.

[0047] Preparation method: 5% Bacillus subtilis XL13 strain is inoculated into culture medium containing 0.1% ground taro powder (content: 1 g / L). Medium selection: beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L. The pH value is 7.0-7.2; culture on a shaker at 37°C for 24 hours. The growth was measured with a UV spectrophotometer at a wavelength of 600 mm, and the growth curve of Bacillus subtilis XL13...

Embodiment 2

[0049] Probiotics, polycarbohydrates, and nitrogen sources are selected as in Example 1, and the preparation method is as follows: 5% Bacillus subtilis XL13 is inoculated in a medium containing 2.0% ground taro powder (content: 20g / L). The composition of the medium was as described in Example 1. Samples were taken at 0 hour, 8 hours, 27 hours, 62 hours, and 72 hours, and the reducing sugar content in the fermentation broth was determined by the 3,5-2 nitrosalicylic acid colorimetric method (Applied Microbiology and Biotechnology 26, 323). Enzyme activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar equivalent to D-mannose per minute as 1 enzyme activity unit. The results are shown in Table 1. The highest enzyme activity reaches 162IU / ml.

[0050] Table 1:

[0051] strain

Embodiment 3

[0053] Probiotics, polycarbohydrates, and carbon sources are selected as in Example 1, and the preparation method is as follows: 5% Bacillus licheniformis XL42 is inoculated in a medium containing 2.0% taro powder. The medium components are as described in Example 1. Samples were taken at 0 hour, 8 hours, 27 hours, 62 hours, and 72 hours, and the reducing sugar content in the fermentation broth was determined by the 3,5-2 nitrosalicylic acid colorimetric method (Applied Microbiology and Biotechnology 26, 323). Enzyme activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar equivalent to D-mannose per minute as 1 enzyme activity unit. The results are shown in Table 2. The highest enzyme activity reaches 38IU / ml.

[0054] Table 2:

[0055] strain

[0056] 5% lichen buds

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Abstract

The present invention is one kind of green feed additive. Probiotics strain is used in fermentation to produce beta-mannase; the beta-mannase is used in decomposing polymer carbohydrate to produce oligomannose and mannose; and the mixture of beta-mannase, oligomannose, mannose and probiotics as the green feed additive is finally produced. The said process is simple one-step process of producing the microecological preparation with high feed converting rate.

Description

1. Technical field [0001] The invention relates to a feed additive, especially a feed additive containing oligosaccharides and probiotics and a method for preparing and producing the feed additive. 2. Background technology [0002] People have now realized that the long-term abuse of antibiotics in feed not only weakens the disease resistance of reared animals, reduces the quality and taste of meat and egg products, but also greatly increases the probability of carrying drug resistance factors in pathogenic bacteria. Therefore, it is imperative to develop feed additives without antibiotics. As the most likely green feed additive to replace antibiotics, probiotics have attracted people's attention. It is generally believed that probiotics include probiotics and prebiotics. Commonly used probiotics for livestock and poultry include Bacillus, Lactobacillus, Bifidobacterium, Streptococcus and some strains in photosynthetic cells. Adding probiotics to feed can competitively in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K10/12A23K10/18A23K10/30A23K20/163A23K20/189
Inventor 王欣顾君华
Owner 浙江德清利欣生物科技有限公司
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