Porous material for scaffold of tissue engineering and its preparing process

A tissue engineering scaffold and porous material technology, applied in the field of biomedical materials, can solve the problems of accelerating tissue repair speed, difficulty in adapting to tissue regeneration speed, affecting the shape and function of new tissue, and achieving the effect of uniform structure and performance

Inactive Publication Date: 2003-02-19
苏州苏豪生物材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the tissue regeneration rate is fast, the scaffold material is required to be degraded and absorbed quickly, otherwise it may affect the shape and function of the new tissue, and when the tissue regeneration rate is slow, the scaffold material should actively guide and promote cell growth into a standardized tissue ability, thereby speeding up the speed of tissue repair. At this time, the speed of degradation and absorption of the scaffold material also needs to be accelerated at the same time, so as to adapt to the speed of tissue regeneration. Therefore, the biodegradation speed of porous materials prepared solely with silk fibroin is difficult. Adapt to tissue regeneration rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Silkworm cocoons are obtained by stripping, cutting, and removing pupa to obtain a cocoon layer, after 1 kilogram of clean silkworm cocoons are fully washed with tap water, then washed 3 times with deionized water, put into 20 liters of deionized water, and placed in 98 liters of deionized water. Treat the sericin at ~100°C for 40 minutes to dissolve the sericin. After filtration, an aqueous solution of sericin with a concentration of 1.5% is obtained, and a part of the aqueous sericin solution is lyophilized to obtain solid sericin.

[0020] 2. Put 1 kilogram of silkworm silk into 10 liters of sodium carbonate aqueous solution with a concentration of 0.025%, boil for 0.5 hours, repeat the treatment three times, and remove the sericin around the silk to obtain pure silk fibroin; Fiber, use 10 liters of calcium chloride, water, ethanol solution with a molar ratio of 1:8:2, heat and dissolve it into a silk fibroin mixed solution at 78 ± 2 °C; use cellulose membrane as a...

Embodiment 2

[0028] 1. Put 0.5 kilograms of Chinese tussah silk into 10 liters of sodium carbonate aqueous solution with a concentration of 0.5%, boil for 0.5 hours, repeat the treatment three times, and remove the sericin around the tussah silk to obtain tussah silk fibroin fibers.

[0029] 2. Use the lithium thiocyanide that the concentration is 9M to dissolve the tussah silk at a temperature of 40 ° C and a liquor ratio of 1: 20 to obtain a mixed solution; the above solution is dialyzed against water for 4 days and nights, and the dialysis material is a cellulose membrane with a molecular weight cut-off of 11,000. , to obtain a pure tussah silk fibroin aqueous solution; the above pure tussah silk fibroin solution is maintained at 40° C., and is evaporated and concentrated while stirring, so that the solid content of the solution reaches 10.0%.

[0030] After 3.0.5 kg of clean cocoon layers were washed thoroughly with water, they were put into 12 liters of deionized water and treated at 9...

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PUM

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Abstract

A porous material used as the scaffold in tissue engineering is prepared from the sericine and silk extract extracted from the silk of silkworm or tussah through proportionally mixing, fast stirring at a certain temp. freeze drying to shape porous block or membrane, and injecting the slowly-releasing microgel of cell growth factor into the pores. Its advantages are high biocompatibility and adjustable biodegradation speed.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a porous material suitable for tissue engineering scaffolds and a preparation method thereof. Background technique [0002] At present, biodegradable porous materials for tissue engineering have been developed, mainly including polylactic acid (PLA), polyglycolic acid (PGA), and collagen. However, synthetic materials (PLA, PGA, etc.) still have many problems in biocompatibility, physicochemical properties, control of degradation rate and sustained release, and the synthesis process is complicated. Collagen in natural material itself contains a lot of biological information, which can enable cells to maintain or produce many functions, but the purification of specific types of collagen is cumbersome, quality control is more difficult, and whether the influence of antigens and diseases can be completely avoided. The spread remains to be seen. [0003] Studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L33/04
Inventor 李明忠吴徵宇
Owner 苏州苏豪生物材料科技有限公司
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