HCV and TORCH protein chip and its preparation and application method

Abstract

The invention discloses a chip for diagnosing the proteins. The antigens corresponding to multiple relevant indexes are printed on the different positions of the membrane of cellulose nitrate. The multi-tape immunoblotting method is utilzied through the coloration of gold mark or the precipitation enzyme without coloration to detect the antibody in the specimen of the sufferer aiming at the antibodies all antigens. The invention raises the detecting sensitivity and the specificity of the immunizing method so as to avoid the false negative or the false positive, providing the results for detecting multiple antigens in one one-stroke detection. Since the chip possesses more information than the information in the testing sheet so as to reduce the collecting quantity and shorten the testing period.

Description

technical field [0001] The invention belongs to the field of reagents for in vitro diagnosis in the field of biotechnology and its preparation method and application technology, in particular to a biological chip, in particular to a protein chip. Background technique [0002] A protein chip is a protein or polypeptide array arranged in an orderly manner on a solid-state substrate to achieve parallel detection, identification, identification and diagnosis of proteins. It can be used in biology, medicine and related fields. [0003] In the 21st century, human beings are faced with a series of severe challenges such as population, resources and the environment. Biotechnology has become an important strategic choice to deal with these challenges. It is to solve food, medicine, environmental protection, etc. It is an important technical means to improve the health status of the general public and improve the quality of life, promote the transformation of traditional industries an...

AI Technical Summary

Problems solved by technology

In comparison, this enzyme-labeled technology has higher sensitivity and specificity than gold-labeled technology, but this technology requires experimental equipment such as centrifuges, incubators, and microplate readers, and the operation process is complicated and time-consuming. Only one protein can be detected, and this method must accumulate a certain number of cases before it can be tested, so it cannot be tested anytime and anywhere

[0006] Colloidal gold immunochromatography is a new type of rapid immunological detection technology in the 1990s. All the raw materials required for the reaction have been integrated into the reagent. When the sample to be tested (urine or serum) is added to the sample membrane After being applied, due to the capillary action of the microporous membrane, the antigen-antibody reaction is carried out quickly on the solid-phase membrane, and the reaction usually only takes a few minutes. The result of enrichment interpretation is therefore not as quantitative as the enzyme-labeled method, and only the reaction result of one index can be obtained through one reaction, so its application is limited to a certain extent

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