Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibiotic streptomyces and its preparation method

A technology of antibiotics and streptomyces, applied in the direction of bacteria, etc., can solve the problems of unfavorable one-drug multi-treatment, weak pest population control, and short validity period.

Inactive Publication Date: 2003-09-24
WUHAN EAST LAKE HIGH TECH GRP CO LTD
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bio-derived pesticides have been fully developed and widely used, but there are still some outstanding problems and difficulties in their research, development, production and application, which hinder the development of bio-derived pesticides in China
For example, the knockdown speed of biological source pesticides is slow, the validity period is short, and the relative insecticidal spectrum is relatively narrow. The control of pest populations is not strong in the short term, and the population density of pests cannot be quickly reduced.
Compared with the narrow insecticidal spectrum, it is not conducive to multi-treatment with one drug. For example, when multiple pests appear in the vegetable field, they will lose sight of the other and be unable to do what they want. It is difficult to apply and promote

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0089] 1. Isolation and cultivation of strains

[0090] Before separation, the soil samples were treated with high temperature dry heat at 120°C for one hour, and then two separation methods were used for pretreatment: 1) 6% yeast extract was added to 5umol phosphate buffer, and treated at 40°C for 20 minutes; 2) 6% Yeast extract, 0.1% SDS, 2mgNA (nalidixic acid) treatment. Nutrient agar was used as the separation medium, and 20ppm NA or 50ppm potassium dichromate was added as a selective inhibitor, cultured at 28°C, single colonies with special morphology and no pollution were selected for 3-7 days, and finally cultivated on No. 38 medium.

[0091] (1) Separation medium 1: soluble starch 1%, casein 0.03%, potassium nitrate 0.2%, magnesium sulfate heptahydrate 0.005%, sodium chloride 20%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.002%, iron sulfate 0.001%, water 78%, pH7.0; culture temperature 28°C.

[0092] (2) Isolation medium 2 (ISP5): L-asparagine 0.1%, gl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a new antibiotic streptomyces daliensis sp.nov. CCTCC No.M201031 and its preparation method. Its preparation method includes the following steps: drying and pretreating soil sample, using sterile water to dilute concentration, coating on separation culture medium, inverted culture in calorsat, selecting single colony, culture on culture medium, shake-flask fermentation of separated pure strain, using fermented browh water phase and fast phase sample to make biological screening, mixing the water sample, artificial fued and target insect ovum and making culture.Said invented has high activity for killing insects, can be used for killing bollworm, asparagus fern caterpillar, cabbageworm and pine moth, etc.

Description

technical field [0001] The invention relates to a new antibiotic streptomyces (Streptomyces daliensissp.nov.) with insecticidal activity, and also relates to a preparation method of the antibiotic streptomyces. Background technique [0002] With the wide application of chemical pesticides in the control of crop diseases and insect pests, the environmental pollution and public hazards caused by them are also becoming more and more serious. Moreover, the accumulation of pesticides in the food chain caused by chemical pesticide residues is not easy to control, resulting in a large number of drug-induced diseases, reducing people's quality of life and damaging the health of the people. In terms of earning foreign exchange through agricultural exports, pesticide residues have also reduced the competitiveness of my country's agricultural and sideline products. At the same time, the use of a large number of chemical pesticides has led to the emergence of dru...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20
Inventor 谢天键姜成林刘华梅李文军杨和文张述谢翔季琦晔
Owner WUHAN EAST LAKE HIGH TECH GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com