Medusa cystatin gene, and expression and use thereof

Abstract

A cystatin gene of meduse, its expression, and its use in suppressing the hydrolysis of protease are disclosed. The protein coded by said gene is a precursor protein composed of 131 amino acids, including the signal peptide containing 18 amino acid and the mature protein containing 113 amino acid. The purified recombinant protein has obvious suppression action on papain.

Description

technical field

[0001] The invention relates to a new gene, especially a jellyfish cysteine ​​protease inhibitor gene; the invention also relates to the expression of the gene and the inhibitory effect of the encoded protein on papain. Background technique

[0002] Protease inhibitors are a kind of water molecular protein that has inhibitory activity on proteolytic enzymes, and are ubiquitous in plants, animals and microorganisms. In vertebrates, protease inhibitors are an integral part of the immune system and act by combining their active centers with proteases to form stable complexes. There are few studies on protease inhibitors in invertebrates. It is worth noting that there is no immune system in the body, so the role of protease inhibitors cannot be ignored. Protease inhibitors are classified according to the type of protease they act on, and are mainly divided into: serine protease inhibitors that inhibit trypsin, chymotrypsin, etc.; cysteine ​​protease inhibitors t...

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