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Method for extracting and separating 1,3-propylene glycol from microbial fermented liquor

A microbial fermentation liquid and microbial fermentation technology, applied in the field of separation 1, can solve the problems of small separation capacity, hindering the evaporation of fractions, and high cost, and achieve the effects of reducing losses, reducing costs, and saving water resources

Inactive Publication Date: 2003-12-10
DALIAN UNIV OF TECH
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Problems solved by technology

[0004] The purpose of the present invention is to solve some problems in the process of producing 1,3-propanediol follow-up separation and purification products by fermentation method, such as high cost, small separation capacity, salt crystallization and viscous substances hindering fraction evaporation in the later stage of vacuum distillation, etc. problem, and propose a kind of effective method for separating 1,3-propanediol from fermented liquid, make microbial fermentation method produce 1,3-propanediol technically, economically feasible

Method used

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Embodiment

[0025] The fermentation broth used in this example is obtained by using Klebsiella pneumoniae to produce 1,3-propanediol by batch fed glycerol fermentation, wherein the concentration of 1,3-propanediol is 63 g / l.

[0026] Klebsiella (K. pneumoniae) was purchased from the China Collection of Common Microorganisms (CGMCC), and its collection number is 1.1736.

[0027] The separation process is divided into five steps:

[0028] 1) Take 350ml of fermentation broth and distill under reduced pressure on a rotary evaporator, the vacuum degree is 0.088MPa, the temperature is controlled ≤75℃, and it is concentrated to 60~80ml;

[0029] 2) After standing and cooling to room temperature, add 140ml of 95% industrial ethanol, stir for 15 minutes and then stand for 5 hours, then centrifuge at 3000rpm for 10 minutes, and take the supernatant;

[0030] 3) Add 70 ml of 95% industrial ethanol to the precipitate, stir for 15 minutes, centrifuge at 3000 rpm for 10 minutes, and take the supernatant;

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Abstract

The present invention belongs to the field of biological engineering technology, in particular, it relates to a method for extracting and separating 1,3-propanediol from fermented liquor. It includes the following steps: directly distilling and concentrating fermented liquor with bacteria to 1 / 2-1 / 20 of original volume, cooling, adding ethyl alcohol or methyl alcohol, n-propyl alcohol, isopropyl alcohol, acetone or butanone according to volume ratio of 1:0.3-1:5, settling or filtering or 1000-4500 rpm centrifugal separation and precipitation, taking supernatant fluid, then using alcohol or ketone to wash and precipitating, then settling or filtering or 1000-1500 rpm centrifugal separation and precipitation, taking supernatant fluid, then recovering alcohol or ketone from said supernatant fluid by means of distillation or rectification.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the separation technology of microbial fermentation broth, in particular to a method for separating 1,3-propanediol from the microbial fermentation broth. Background technique [0002] As we all know, 1,3-propanediol is an important chemical raw material. It has the same use as ethylene glycol, 1,2-propanediol and 1,4-butanediol, but it is a new type of polyester synthesized with terephthalic acid. Polytrimethylene terephthalate (PTT) has excellent properties that many polyester materials do not have, such as nylon-like elastic recovery, UV resistance, ozone and nitrogen oxide coloration, low static electricity, low water absorption, In the full color range, it does not need to add any special chemicals and presents good continuous printing and dyeing properties and biodegradability. These all show the bright application prospects of 1,3-propanediol, but its expensive price hinde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/76C07C31/20C12P7/18
Inventor 修志龙张代佳高素军陈曦
Owner DALIAN UNIV OF TECH
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