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Targeted anticarious DNA vaccine and peparation thereof

A DNA vaccine, anti-caries technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, digestive system, etc.

Inactive Publication Date: 2004-04-21
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Yang Jinbo, Zhou Xuedong, Liu Tianjia, Construction of Streptococcus mutans glucosyltransferase eukaryotic expression plasmid PcDNA3-gtfB, West China Journal of Stomatology, 2001: 19(4): 249-252) However, anti-caries DNA vaccines and other DNA Vaccines still face the problem of how to improve immune efficacy

Method used

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  • Targeted anticarious DNA vaccine and peparation thereof
  • Targeted anticarious DNA vaccine and peparation thereof
  • Targeted anticarious DNA vaccine and peparation thereof

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Embodiment Construction

[0045] The present invention is described in further detail below according to the accompanying drawings:

[0046] according to figure 1 It can be seen that the use of molecular biology professional software: DNASIS comprehensive DNA sequence analysis software, Gene Construction kit 2.0 gene simulation cloning software, Omiga comprehensive molecular biological analysis software, Oligo primer design analysis software and other software to analyze and design targeted anti-caries DNA vaccines and simulate The whole process of cloning.

[0047] according to figure 2 It can be seen that a pair of aptamers are designed, respectively: aptamer 1: 5'TCGACTAAACGCGTGC3', aptamer 2: 5'GGCCGCACGCGTTTAG3', SalI and NotI sites are designed at the 5' end and 3' respectively, and MluI is designed in the middle. site, the aptamer was pre-annealed; pGJA-P was double digested with SalI and NotI, and the target fragment containing part of the vector sequence and CTLA4, Ig, and GLU was recovered...

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Abstract

A targeting anticariogenic DNA vaccine pGJGLU is prepared from Esherichia coli JM109 / pGJGLU CCTCC No.M202036 through designing a pair of adaptors, enzyme servering of target fused anticariogenic DNA vaccine pGJA-P by use of SalI and NotI to remove A-P fragment, linking reacting by use of ligase T4, and configuring said DNA vaccine pGJGLU. Its advantages are simple process, high safety, low cost, and durable immunoresponse.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and more particularly relates to a sequence encoding human cytotoxic T lymphocyte antigen 4 signal peptide, extracellular region and Igγ 1 A DNA anti-caries vaccine of hinge region, constant region fusion gene and Streptococcus mutans glucosyltransferase gene, and the invention also relates to a preparation method of the targeted anti-caries DNA vaccine. Background technique [0002] Caries is a bacterial infection. Many scholars have proved that Streptococcus mutans (Streptococcus mutans) is the most important pathogenic bacteria causing human caries. glucosyltransferase (glucosyltransferasesGTF) s ) is an important cariogenic virulence factor of Streptococcus mutans. GTF s Synthesizes glucan and mediates glucan-dependent adhesion of Streptococcus mutans to tooth surfaces. GTF s Contains two immunoactive regions and functional regions: catal...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61K45/00A61P1/02
CPCY02A50/30
Inventor 樊明文边专贾荣郭继华陈智彭彬范兵
Owner WUHAN UNIV
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