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Technique for preparing 5'nucleotide bi-sodium

A technology for the preparation of disodium nucleotides, which is applied in the field of preparation of strong freshness aids, can solve the problems of cumbersome operation, impact on yield, poor 5'-position selectivity, etc., and reduce consumption and pollution , the effect of a simple post-processing process

Inactive Publication Date: 2004-10-27
SHANGHAI ZHAOWEI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Among the above processes, 1 is a relatively classic acylation reaction process, the selectivity to the 5'-position is not good, and the yield is affected
Using pyridine, triethylamine, etc. as catalysts, these compounds have excellent water solubility, so they will cause serious water pollution
2 is to first protect the 2'- and 3'-position hydroxyl groups of nucleosides, then carry out the phosphorylation of the 5'-position, and finally deprotection, the steps are cumbersome
However, the above methods are cumbersome and time-consuming, and the regeneration of the separation column will generate a large amount of waste water.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Add 50 grams of inosine, 500 grams of triethyl phosphate, and 3.4 grams of water into the reaction kettle, stir to cool down, add 87.5 grams of phosphorus oxychloride dropwise at about 5°C, and continue the reaction for 1.5 hours after dropping;

[0028] Cool down 900 grams of 10% sodium chloride solution to below 0°C, pour the above-mentioned reaction solution into it for hydrolysis while stirring, and then stand and separate for 20-60 minutes after 1 hour;

[0029] The hydrolyzed solution is naturally divided into triethyl phosphate and aqueous solution layer, the aqueous solution layer is cooled with 50% sodium hydroxide solution to adjust to PH ≥ 13, crystallized at 0 ℃ for 2-3 hours, and filtered;

[0030] The filtered solid is mainly sodium phosphate, the filtrate is neutralized with concentrated hydrochloric acid to PH=8-9, concentrated under reduced pressure to about 600 g remaining, cooled to 0-5°C, and filtered;

[0031] The filtered solid was crude disodium inosi...

Embodiment 2

[0033] 26.8 grams of inosine, 32.5 grams of guanosine, 650 grams of triethyl phosphate, and 3.6 grams of water were added to the reaction kettle, the temperature was lowered, 92 grams of phosphorus oxychloride was added dropwise at about 5°C, and the reaction was continued for 3 hours after dropping;

[0034] The temperature of 1000 grams of 10% sodium chloride solution is lowered to below 0°C, the above reaction liquid is poured into it for hydrolysis under stirring, and then it is allowed to stand for layering after 1 hour;

[0035] The hydrolyzed solution is naturally divided into a triethyl phosphate layer and an aqueous solution layer. The aqueous layer is cooled with 50% sodium hydroxide solution to adjust to PH≥13, crystallized at 0°C for 2-3 hours, and filtered;

[0036] The filtered solid is mainly sodium phosphate, the filtrate is neutralized with concentrated hydrochloric acid to PH=8-9, concentrated under reduced pressure to about 650 g remaining, cooled to 0-5°C, and ...

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Abstract

A process for preparing 5'-nucleotide bisodium includes phosphoacetylation reaction of nucleoside in trialkyl phosphate, hydrolyzing the resultant liquid in ice saline to obtain the hydrolytic liquid containing trialkyl phosphate, water, phosphoric acid, hydrochloric acid and 5'-nucleotide, etc. laying a side for natural layering to obtain trialkyl phosphate layer and the aqueous solution layer containing phosphoric acid, hydrochloride acid, 5'-nucleotide, etc, using sodium hydroxide to neutralize said aqueous solution layer until all acids become salts, cooling, filter, using hydrochloric acid to regulate pH=8, and concentrating-crystallizing.

Description

Technical field [0001] The present invention is a preparation process of a powerful freshness assistant, and specifically relates to a simple and feasible post-treatment process of the preparation process of 5'-nucleotide disodium. Background technique [0002] Nucleotides and their derivatives have been widely used in medicine, food, agriculture and other fields. Both 5'-inosinic acid disodium and 5'-guanylic acid disodium are powerful freshness aids. When they are used in mixed MSG, their umami taste is 40-100 times higher than general MSG, and the flavor is better. After the product was put on the market, it was welcomed by consumers. Disodium cytidine and disodium uridine can be used to supplement the nucleic acid in cow's milk to produce breast-emulsified milk close to human milk, which can enhance the baby's immunity. [0003] As mentioned above, 5'-nucleotide disodium is very important in the fields of food and medicine, and its preparation proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/20
Inventor 金抒徐昌洪
Owner SHANGHAI ZHAOWEI TECH DEV