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Schizophyllum commune artificial cultivation and breeding technology

A technology of artificial cultivation and white ginseng bacteria, which is applied in the fields of fungi, horticulture, botanical equipment and methods, etc., and can solve the problems of multiple mineral varieties, repeated elements, and inhibition of bacterial strains

Inactive Publication Date: 2004-12-29
腾冲县中和乡农民文化技术学校
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. White ginseng fungus is a typical aerobic fungus. Its growth is inseparable from sulfur element. However, if the amount of sulfur is too large, the amount of sulfur decomposed by the bacteria will be large, and free sulfur will easily form a large amount of hydrogen sulfide gas. Inhibit the growth of the strain, or even kill the strain
[0007] 4. In this patent application, 10-20% of cornmeal is used. The dosage is too high, which can easily cause the infection of miscellaneous bacteria;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the operating method of making 1000 square meters of culture medium test tube parent species

[0023] In the season when wild white ginseng grows in large quantities, go to the field to select white ginseng strains that are vigorous, large, normal in color, and free from diseases and insect pests, and use PDA, V B The comprehensive formula is used as the culture medium. Weigh 200g of peeled Maling potatoes by weight, finely crush them, boil them with 1000ml of water, filter the juice through 4 to 6 layers of gauze, add water to the juice to make a total of 1000ml, put it in a pot and heat it to 90 ℃; at the same time weigh 20g of glucose, 3g of potassium dihydrogen phosphate, compound V B 10g, magnesium sulfate 1.5g, peptone 2g, agar 20g, add to the pot and stir, after being dissolved, put it into a test tube while it is hot, put the liquid-filled test tube into a pressure cooker and sterilize it at 117 kPa, 123°C for 45 minutes, After the natural decompr...

Embodiment 2

[0024] Embodiment 2: the operating method of making 100Kg wheat germ classification

[0025] Weigh 98Kg of selected wheat or corn kernels and wash them with water, add 0.5Kg of calcium carbonate and soak for 12 hours to make them fully absorb water, then filter out the wheat kernels or corn kernels and boil them in water for about 15 minutes to make them cooked and If it is not rotten, take it out, add 1.5Kg of calcium sulfate and stir evenly until the hands are not wet. At this time, the water content is about 70%, put it into a 15×30cm special strain bag or bottle, and put it under high temperature and pressure at 147 kPa and 128°C Sterilize after 2.5 hours, and cool down naturally. When the temperature drops below 30°C, in the aseptic room, the bagged ones are inoculated with the near-end dislocation inoculation method; the bottled ones are inoculated deep, which is conducive to the growth of mycelium uniform. After inoculation, cultivate under normal temperature and 10-20...

Embodiment 3

[0026] Embodiment 3: make 1000 bags of cultivars

[0027] The size of the seed bag is 15~17×55~57cm polypropylene or polyethylene long belly bag, each bag requires 1Kg of material, and a total of 1 dry kilogram of mixed material is required. Prepare cultivar culture medium first, take by weighing 680Kg of crushed corncob powder, 200Kg of miscellaneous wood sawdust, 200Kg of bran, 1Kg of calcium carbonate, 1Kg of sugar, and 1300Kg of clear water for later use.

[0028]Take 400Kg corn cob powder and 100Kg miscellaneous sawdust and stir, add 100Kg bran and stir, add 1Kg calcium carbonate and stir again, then add 280Kg corn cob flour, 100Kg miscellaneous sawdust, 100Kg bran respectively. Take 10Kg of water to completely dissolve 1Kg of brown sugar, then add 100Kg of water to dilute it and add it to the mixture for stirring. Gradually add the remaining water to the mixture to keep the water content at 50-55%. Stir well and pack it mechanically . Move it into the atmospheric press...

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Abstract

The artificially cultivation and propagation technology of Schizophyllum commune Fr. includes tissue culture to purify and obtain wild strain of Schizophyllum commune Fr., artificially cultivation in combined culture material, domesticating, propagation, tissue separation and other steps. During the cultivation, the temperature, humidity, air flow and scattered sunlight are artificially controlled so as to produce large, high quality and high yield Schizophyllum commune Fr.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of edible fungi, in particular to an artificial cultivation and breeding technology of white ginseng fungi. Background technique [0002] White ginseng fungus, also known as Rudanchai, belongs to Basidiomycotina, Laminomycetes, Agaricales, and Schizophyllaceae in taxonomy. Wild white ginseng fungus is very strict in the selection of external living and growth conditions, and has strong seasonality. It has wide adaptability to temperature and can grow in the range of 8°C to 30°C, while 16°C to 24°C is the most suitable. Optimum temperature; it has strict requirements on humidity. For example, when the air humidity reaches above 85%, it only takes seven days for the white ginseng fruiting bodies to complete from differentiation to maturity. If the air humidity is lower than 65%, the fruiting bodies will be That is, dehydration and death. When it is found that it is dehydrated, it wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 段会昌余家贵
Owner 腾冲县中和乡农民文化技术学校
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